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Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora

BACKGROUND & OBJECTIVES: Lactobacilli species that are better adapted to vaginal environment of women may colonize better and offer protection against vaginal pathogenic bacteria. In this study, the distribution of common Lactobacillus species was investigated in pregnant women. METHODS: Sixty s...

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Autores principales: Yeruva, Thirupathaiah, Rajkumar, Hemalatha, Donugama, Vasundhara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819037/
https://www.ncbi.nlm.nih.gov/pubmed/29434069
http://dx.doi.org/10.4103/ijmr.IJMR_774_16
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author Yeruva, Thirupathaiah
Rajkumar, Hemalatha
Donugama, Vasundhara
author_facet Yeruva, Thirupathaiah
Rajkumar, Hemalatha
Donugama, Vasundhara
author_sort Yeruva, Thirupathaiah
collection PubMed
description BACKGROUND & OBJECTIVES: Lactobacilli species that are better adapted to vaginal environment of women may colonize better and offer protection against vaginal pathogenic bacteria. In this study, the distribution of common Lactobacillus species was investigated in pregnant women. METHODS: Sixty seven pregnant women were included in the study and vaginal samples were collected for Gram staining. Women were classified as normal vaginal flora, intermediate flora and bacterial vaginosis (BV) based on Nugent's score. Vaginal samples were also collected for the identification of Lactobacillus spp. by multiplex polymerase chain reaction (PCR) profiling of 16S rDNA amplification method. RESULTS: Lactobacillus crispatus (100%) was the most predominant Lactobacillus spp. present in pregnant women with normal flora, followed by L. iners (77%), L. jensenii (74%) and L. helveticus (60%). While, L. iners was commonly present across groups in women with normal, intermediate or BV flora, L. crispatus, L. jensenii and L. helveticus decreased significantly as the vaginal flora changed to intermediate and BV. In women with BV, except L. iners other species of lactobacilli was less frequently prevalent. Species such as L. rhamnosus, L. fermentum, L. paracasei and L. casei were not detected in any vaginal sample. INTERPRETATION & CONCLUSIONS: L. crispatus, L. jensinii and L. helveticus were predominant species in women with normal flora. L. crispatus alone or in combination with L. jensinii and L. helveticus may be evaluated for probiotic properties for the prevention and treatment of BV.
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spelling pubmed-58190372018-02-22 Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora Yeruva, Thirupathaiah Rajkumar, Hemalatha Donugama, Vasundhara Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Lactobacilli species that are better adapted to vaginal environment of women may colonize better and offer protection against vaginal pathogenic bacteria. In this study, the distribution of common Lactobacillus species was investigated in pregnant women. METHODS: Sixty seven pregnant women were included in the study and vaginal samples were collected for Gram staining. Women were classified as normal vaginal flora, intermediate flora and bacterial vaginosis (BV) based on Nugent's score. Vaginal samples were also collected for the identification of Lactobacillus spp. by multiplex polymerase chain reaction (PCR) profiling of 16S rDNA amplification method. RESULTS: Lactobacillus crispatus (100%) was the most predominant Lactobacillus spp. present in pregnant women with normal flora, followed by L. iners (77%), L. jensenii (74%) and L. helveticus (60%). While, L. iners was commonly present across groups in women with normal, intermediate or BV flora, L. crispatus, L. jensenii and L. helveticus decreased significantly as the vaginal flora changed to intermediate and BV. In women with BV, except L. iners other species of lactobacilli was less frequently prevalent. Species such as L. rhamnosus, L. fermentum, L. paracasei and L. casei were not detected in any vaginal sample. INTERPRETATION & CONCLUSIONS: L. crispatus, L. jensinii and L. helveticus were predominant species in women with normal flora. L. crispatus alone or in combination with L. jensinii and L. helveticus may be evaluated for probiotic properties for the prevention and treatment of BV. Medknow Publications & Media Pvt Ltd 2017-10 /pmc/articles/PMC5819037/ /pubmed/29434069 http://dx.doi.org/10.4103/ijmr.IJMR_774_16 Text en Copyright: © 2018 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Yeruva, Thirupathaiah
Rajkumar, Hemalatha
Donugama, Vasundhara
Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora
title Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora
title_full Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora
title_fullStr Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora
title_full_unstemmed Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora
title_short Vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora
title_sort vaginal lactobacilli profile in pregnant women with normal & abnormal vaginal flora
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819037/
https://www.ncbi.nlm.nih.gov/pubmed/29434069
http://dx.doi.org/10.4103/ijmr.IJMR_774_16
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