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Aberration-corrected cryoimmersion light microscopy

Cryogenic fluorescent light microscopy of flash-frozen cells stands out by artifact-free fixation and very little photobleaching of the fluorophores used. To attain the highest level of resolution, aberration-free immersion objectives with accurately matched immersion media are required, but both do...

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Detalles Bibliográficos
Autores principales: Faoro, Raffaele, Bassu, Margherita, Mejia, Yara X., Stephan, Till, Dudani, Nikunj, Boeker, Christian, Jakobs, Stefan, Burg, Thomas P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819432/
https://www.ncbi.nlm.nih.gov/pubmed/29358380
http://dx.doi.org/10.1073/pnas.1717282115