Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus)

ABSTRACT: The genetic markers designed for this study can facilitate future genetic studies on the rock ptarmigan (Lagopus muta). To our knowledge no microsatellite markers have ever been developed specifically for this species before. These new microsatellite markers will be useful for population g...

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Autores principales: Costanzi, Jean-Marc, Bergan, Frode, Sæbø, Mona, Jenkins, Andrew, Steifetten, Øyvind
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819644/
https://www.ncbi.nlm.nih.gov/pubmed/29463303
http://dx.doi.org/10.1186/s13104-018-3249-1
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author Costanzi, Jean-Marc
Bergan, Frode
Sæbø, Mona
Jenkins, Andrew
Steifetten, Øyvind
author_facet Costanzi, Jean-Marc
Bergan, Frode
Sæbø, Mona
Jenkins, Andrew
Steifetten, Øyvind
author_sort Costanzi, Jean-Marc
collection PubMed
description ABSTRACT: The genetic markers designed for this study can facilitate future genetic studies on the rock ptarmigan (Lagopus muta). To our knowledge no microsatellite markers have ever been developed specifically for this species before. These new microsatellite markers will be useful for population genetics studies and for future conservation projects. RESULTS: Using Next Generation Sequencing 6252 potential microsatellite sequences were found. Sixteen nonpalindromic tetranucleotide microsatellites and their respective primers were selected. The markers were tested on both the rock ptarmigan and the willow grouse (L. lagopus). The number of alleles varied between 2 and 18 for the rock ptarmigan, and between 3 and 13 for the willow grouse. Expected heterozygosity was in the range 0.1244–0.8692 and 0.1358–0.8722 for the rock ptarmigan and the willow grouse, respectively.
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spelling pubmed-58196442018-02-26 Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus) Costanzi, Jean-Marc Bergan, Frode Sæbø, Mona Jenkins, Andrew Steifetten, Øyvind BMC Res Notes Research Note ABSTRACT: The genetic markers designed for this study can facilitate future genetic studies on the rock ptarmigan (Lagopus muta). To our knowledge no microsatellite markers have ever been developed specifically for this species before. These new microsatellite markers will be useful for population genetics studies and for future conservation projects. RESULTS: Using Next Generation Sequencing 6252 potential microsatellite sequences were found. Sixteen nonpalindromic tetranucleotide microsatellites and their respective primers were selected. The markers were tested on both the rock ptarmigan and the willow grouse (L. lagopus). The number of alleles varied between 2 and 18 for the rock ptarmigan, and between 3 and 13 for the willow grouse. Expected heterozygosity was in the range 0.1244–0.8692 and 0.1358–0.8722 for the rock ptarmigan and the willow grouse, respectively. BioMed Central 2018-02-20 /pmc/articles/PMC5819644/ /pubmed/29463303 http://dx.doi.org/10.1186/s13104-018-3249-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Costanzi, Jean-Marc
Bergan, Frode
Sæbø, Mona
Jenkins, Andrew
Steifetten, Øyvind
Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus)
title Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus)
title_full Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus)
title_fullStr Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus)
title_full_unstemmed Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus)
title_short Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus)
title_sort development and evaluation of 16 new microsatellite loci for the rock ptarmigan (lagopus muta) and cross-species amplification for the willow grouse (l. lagopus)
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819644/
https://www.ncbi.nlm.nih.gov/pubmed/29463303
http://dx.doi.org/10.1186/s13104-018-3249-1
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