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Evaluation of Stability of Thiopurine Metabolites Using a Validated LC-MS/MS Method
Measurement of thiopurine metabolites is helpful to monitor adverse effects and assess compliance in patients on thiopurine treatment. The purpose of this study was to develop and validate an analytical method for measurement of thiopurine metabolites, thioguanine nucleotides (6-TGN) and 6-methylmer...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Society for Laboratory Medicine
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820071/ https://www.ncbi.nlm.nih.gov/pubmed/29401561 http://dx.doi.org/10.3343/alm.2018.38.3.255 |
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author | Yoo, In-Young Lee, Kyunghoon Ji, Ok-Ja Woo, Hye In Lee, Soo-Youn |
author_facet | Yoo, In-Young Lee, Kyunghoon Ji, Ok-Ja Woo, Hye In Lee, Soo-Youn |
author_sort | Yoo, In-Young |
collection | PubMed |
description | Measurement of thiopurine metabolites is helpful to monitor adverse effects and assess compliance in patients on thiopurine treatment. The purpose of this study was to develop and validate an analytical method for measurement of thiopurine metabolites, thioguanine nucleotides (6-TGN) and 6-methylmercaptopurine nucleotide (6-MMPN), in RBCs. We developed and validated a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the quantification of 6-TGN and 6-MMPN and evaluated the stability of the thiopurine metabolites in RBC and whole blood states without any preprocessing at various storage conditions. The linear range was 0.1–10 µmol/L and 0.5–100 µmol/L for 6-TGN and 6-MMPN, respectively. The mean extraction recovery at the two concentrations was 71.0% and 75.0% for 6-TGN, and 102.2% and 96.4% for 6-MMPN. Thiopurine metabolites in preprocessed RBC samples were stable at 25℃ and 4℃ after storage for 4 hours and at −70℃ for up to 6 months. However, 6-TGN decreased by 30% compared with the initial concentration when stored at −20℃ for 180 days. In whole blood states, 6-TGN decreased by about 20% at four days after storage at 4℃. We validated a reliable LC-MS/MS method and recommend that the patient's whole blood sample be preprocessed as soon as possible. |
format | Online Article Text |
id | pubmed-5820071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Korean Society for Laboratory Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-58200712018-05-01 Evaluation of Stability of Thiopurine Metabolites Using a Validated LC-MS/MS Method Yoo, In-Young Lee, Kyunghoon Ji, Ok-Ja Woo, Hye In Lee, Soo-Youn Ann Lab Med Brief Communication Measurement of thiopurine metabolites is helpful to monitor adverse effects and assess compliance in patients on thiopurine treatment. The purpose of this study was to develop and validate an analytical method for measurement of thiopurine metabolites, thioguanine nucleotides (6-TGN) and 6-methylmercaptopurine nucleotide (6-MMPN), in RBCs. We developed and validated a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the quantification of 6-TGN and 6-MMPN and evaluated the stability of the thiopurine metabolites in RBC and whole blood states without any preprocessing at various storage conditions. The linear range was 0.1–10 µmol/L and 0.5–100 µmol/L for 6-TGN and 6-MMPN, respectively. The mean extraction recovery at the two concentrations was 71.0% and 75.0% for 6-TGN, and 102.2% and 96.4% for 6-MMPN. Thiopurine metabolites in preprocessed RBC samples were stable at 25℃ and 4℃ after storage for 4 hours and at −70℃ for up to 6 months. However, 6-TGN decreased by 30% compared with the initial concentration when stored at −20℃ for 180 days. In whole blood states, 6-TGN decreased by about 20% at four days after storage at 4℃. We validated a reliable LC-MS/MS method and recommend that the patient's whole blood sample be preprocessed as soon as possible. The Korean Society for Laboratory Medicine 2018-05 2018-02-02 /pmc/articles/PMC5820071/ /pubmed/29401561 http://dx.doi.org/10.3343/alm.2018.38.3.255 Text en © The Korean Society for Laboratory Medicine http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Communication Yoo, In-Young Lee, Kyunghoon Ji, Ok-Ja Woo, Hye In Lee, Soo-Youn Evaluation of Stability of Thiopurine Metabolites Using a Validated LC-MS/MS Method |
title | Evaluation of Stability of Thiopurine Metabolites Using a Validated LC-MS/MS Method |
title_full | Evaluation of Stability of Thiopurine Metabolites Using a Validated LC-MS/MS Method |
title_fullStr | Evaluation of Stability of Thiopurine Metabolites Using a Validated LC-MS/MS Method |
title_full_unstemmed | Evaluation of Stability of Thiopurine Metabolites Using a Validated LC-MS/MS Method |
title_short | Evaluation of Stability of Thiopurine Metabolites Using a Validated LC-MS/MS Method |
title_sort | evaluation of stability of thiopurine metabolites using a validated lc-ms/ms method |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820071/ https://www.ncbi.nlm.nih.gov/pubmed/29401561 http://dx.doi.org/10.3343/alm.2018.38.3.255 |
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