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Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L.

Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs). The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated. The ACPs we...

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Autores principales: Shang, Hongmei, Wang, Menghan, Li, Ran, Duan, Mengying, Wu, Hongxin, Zhou, Haizhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820361/
https://www.ncbi.nlm.nih.gov/pubmed/29463789
http://dx.doi.org/10.1038/s41598-018-21295-z
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author Shang, Hongmei
Wang, Menghan
Li, Ran
Duan, Mengying
Wu, Hongxin
Zhou, Haizhu
author_facet Shang, Hongmei
Wang, Menghan
Li, Ran
Duan, Mengying
Wu, Hongxin
Zhou, Haizhu
author_sort Shang, Hongmei
collection PubMed
description Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs). The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated. The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods. The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained. HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical. The three ACPs exhibited antioxidant abilities in a concentration-dependent manner. ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content. At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively. Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides.
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spelling pubmed-58203612018-02-26 Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L. Shang, Hongmei Wang, Menghan Li, Ran Duan, Mengying Wu, Hongxin Zhou, Haizhu Sci Rep Article Response surface methodology (RSM) including three variables was performed to optimize the extraction parameters of Astragalus cicer L. polysaccharides (ACPs). The influence of different drying techniques on the physicochemical properties and antioxidant abilities of ACPs were evaluated. The ACPs were dried with hot air (HD), vacuum (VD) and freeze drying (FD) methods. The optimal conditions for ACPs extraction were as follows: water to raw material ratio of 25 mL/g, extraction time of 61 min and temperature of 75 °C. Under these parameters, an ACPs yield of 10.97% was obtained. HPLC analysis showed that the monosaccharide compositions of the three ACPs dried with HD, VD or FD techniques were identical. The three ACPs exhibited antioxidant abilities in a concentration-dependent manner. ACPs dried with the FD method (FD-ACPs) had the best antioxidant activities, which might be related to their smaller molecular weight and higher uronic acid content. At the determined concentration of 1 mg/mL, the ferric reducing power, and DPPH and ABTS free radical scavenging capacities of FD-ACPs were 0.762, 75.30% and 99.21%, respectively. Therefore, FD was a good choice for the drying of Astragalus cicer L. polysaccharides. Nature Publishing Group UK 2018-02-20 /pmc/articles/PMC5820361/ /pubmed/29463789 http://dx.doi.org/10.1038/s41598-018-21295-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shang, Hongmei
Wang, Menghan
Li, Ran
Duan, Mengying
Wu, Hongxin
Zhou, Haizhu
Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L.
title Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L.
title_full Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L.
title_fullStr Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L.
title_full_unstemmed Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L.
title_short Extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from Astragalus cicer L.
title_sort extraction condition optimization and effects of drying methods on physicochemical properties and antioxidant activities of polysaccharides from astragalus cicer l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820361/
https://www.ncbi.nlm.nih.gov/pubmed/29463789
http://dx.doi.org/10.1038/s41598-018-21295-z
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