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Etanercept Prevents Histopathological Damage after Spinal Cord Injury in Rats
BACKGROUND: The aim of our study is to assess the neuroprotective effects of the tumor necrosis factor alpha (TNF-α) inhibitor etanercept (ETA) on histopathological and biochemical changes following spinal cord injury (SCI). PATIENTS AND METHODS: Fifty-four male Wistar albino rats were randomly assi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820892/ https://www.ncbi.nlm.nih.gov/pubmed/29492118 http://dx.doi.org/10.4103/ajns.AJNS_307_16 |
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author | Hasturk, Askin Esen Baran, Cagdas Yilmaz, Erdal Resit Arikan, Murat Togral, Guray Hayirli, Nazli Erguder, Berrin Imge Evirgen, Oya |
author_facet | Hasturk, Askin Esen Baran, Cagdas Yilmaz, Erdal Resit Arikan, Murat Togral, Guray Hayirli, Nazli Erguder, Berrin Imge Evirgen, Oya |
author_sort | Hasturk, Askin Esen |
collection | PubMed |
description | BACKGROUND: The aim of our study is to assess the neuroprotective effects of the tumor necrosis factor alpha (TNF-α) inhibitor etanercept (ETA) on histopathological and biochemical changes following spinal cord injury (SCI). PATIENTS AND METHODS: Fifty-four male Wistar albino rats were randomly assigned into three main groups: The sham, trauma, and ETA group (n = 18 per group). Each of these groups was further divided into three subgroups (n = 6 per subgroup) based on the different tissue sampling times postinjury: 1 h, 6 h, and 24 h. Clip compression model was used for SCI. Rats in the ETA group were treated with 5 mg/kg of ETA immediately after the clip was removed. After 1, 6, and 24 h, the spinal cord was totally removed between the levels T8–T10. Sample tissue was immediately harvested and fixed for histopathological and electron microscopic examination and were analyzed for TNF-α, interleukin-1β (IL-1β), superoxide dismutase (SOD), adenosine deaminase, catalase (CAT), and malondialdehyde levels in both the tissue and serum. RESULTS: The serum and tissue levels of cytokines and enzymes were seen to change after SCI between hyperacute, acute, and subacute stages. Treatment with ETA selectively inhibited TNF-α, and IL-1β expression together with increased levels of antioxidative enzymes (SOD, CAT). CONCLUSION: Early administration of ETA after SCI may remarkably attenuate neuronal injury by decreasing tissue and serum TNF-α and IL-1β levels, while increasing antioxidative enzymes such as SOD and CAT in subacute and acute stages, respectively. |
format | Online Article Text |
id | pubmed-5820892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-58208922018-02-28 Etanercept Prevents Histopathological Damage after Spinal Cord Injury in Rats Hasturk, Askin Esen Baran, Cagdas Yilmaz, Erdal Resit Arikan, Murat Togral, Guray Hayirli, Nazli Erguder, Berrin Imge Evirgen, Oya Asian J Neurosurg Original Article BACKGROUND: The aim of our study is to assess the neuroprotective effects of the tumor necrosis factor alpha (TNF-α) inhibitor etanercept (ETA) on histopathological and biochemical changes following spinal cord injury (SCI). PATIENTS AND METHODS: Fifty-four male Wistar albino rats were randomly assigned into three main groups: The sham, trauma, and ETA group (n = 18 per group). Each of these groups was further divided into three subgroups (n = 6 per subgroup) based on the different tissue sampling times postinjury: 1 h, 6 h, and 24 h. Clip compression model was used for SCI. Rats in the ETA group were treated with 5 mg/kg of ETA immediately after the clip was removed. After 1, 6, and 24 h, the spinal cord was totally removed between the levels T8–T10. Sample tissue was immediately harvested and fixed for histopathological and electron microscopic examination and were analyzed for TNF-α, interleukin-1β (IL-1β), superoxide dismutase (SOD), adenosine deaminase, catalase (CAT), and malondialdehyde levels in both the tissue and serum. RESULTS: The serum and tissue levels of cytokines and enzymes were seen to change after SCI between hyperacute, acute, and subacute stages. Treatment with ETA selectively inhibited TNF-α, and IL-1β expression together with increased levels of antioxidative enzymes (SOD, CAT). CONCLUSION: Early administration of ETA after SCI may remarkably attenuate neuronal injury by decreasing tissue and serum TNF-α and IL-1β levels, while increasing antioxidative enzymes such as SOD and CAT in subacute and acute stages, respectively. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5820892/ /pubmed/29492118 http://dx.doi.org/10.4103/ajns.AJNS_307_16 Text en Copyright: © 2018 Asian Journal of Neurosurgery http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Hasturk, Askin Esen Baran, Cagdas Yilmaz, Erdal Resit Arikan, Murat Togral, Guray Hayirli, Nazli Erguder, Berrin Imge Evirgen, Oya Etanercept Prevents Histopathological Damage after Spinal Cord Injury in Rats |
title | Etanercept Prevents Histopathological Damage after Spinal Cord Injury in Rats |
title_full | Etanercept Prevents Histopathological Damage after Spinal Cord Injury in Rats |
title_fullStr | Etanercept Prevents Histopathological Damage after Spinal Cord Injury in Rats |
title_full_unstemmed | Etanercept Prevents Histopathological Damage after Spinal Cord Injury in Rats |
title_short | Etanercept Prevents Histopathological Damage after Spinal Cord Injury in Rats |
title_sort | etanercept prevents histopathological damage after spinal cord injury in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820892/ https://www.ncbi.nlm.nih.gov/pubmed/29492118 http://dx.doi.org/10.4103/ajns.AJNS_307_16 |
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