Cargando…
The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells
In previous work we described the decline in the number of splenocytes of mice which during pregnancy and lactation were fed Rhodiola kirilowii. In this work we present the size of individual subpopulations of splenic lymphocytes in these mice. Experiments were performed on adult inbred female Balb/...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Polish Society of Experimental and Clinical Immunology
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820988/ https://www.ncbi.nlm.nih.gov/pubmed/29472809 http://dx.doi.org/10.5114/ceji.2017.72826 |
_version_ | 1783301467074985984 |
---|---|
author | Lewicki, Sławomir Skopińska-Różewska, Ewa Zdanowski, Robert |
author_facet | Lewicki, Sławomir Skopińska-Różewska, Ewa Zdanowski, Robert |
author_sort | Lewicki, Sławomir |
collection | PubMed |
description | In previous work we described the decline in the number of splenocytes of mice which during pregnancy and lactation were fed Rhodiola kirilowii. In this work we present the size of individual subpopulations of splenic lymphocytes in these mice. Experiments were performed on adult inbred female Balb/c mice, 8-9 weeks old, 20-22 g b.m., mated with adult males from the same strain. Females, from when the copulatory plug was noted up to the 28(th) day after delivery, were supplemented daily with lyophilized aqueous (RKW) or 50% hydro-ethanolic (RKW-A) extract (20 mg/kg b.m.) dissolved in distilled water. Then, mice were euthanized, spleens dissected, cells counted and the total numbers of CD3+, CD19+, CD4+, CD8+ and CD335+ splenic lymphocytes were evaluated by cytometry. The number of CD3+ lymphocytes per 1 g of splenic tissue was higher in RKW-A than in RKW spleens and did not differ from the control. The number of CD3+ lymphocytes in RKW spleens was lower than in the controls. The number of CD19+ and CD4+ cells was lower in both experimental groups than in the controls. The number of CD335+(NK) cells was lower in RKW spleens than in the control. |
format | Online Article Text |
id | pubmed-5820988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Polish Society of Experimental and Clinical Immunology |
record_format | MEDLINE/PubMed |
spelling | pubmed-58209882018-02-22 The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells Lewicki, Sławomir Skopińska-Różewska, Ewa Zdanowski, Robert Cent Eur J Immunol Experimental Immunology In previous work we described the decline in the number of splenocytes of mice which during pregnancy and lactation were fed Rhodiola kirilowii. In this work we present the size of individual subpopulations of splenic lymphocytes in these mice. Experiments were performed on adult inbred female Balb/c mice, 8-9 weeks old, 20-22 g b.m., mated with adult males from the same strain. Females, from when the copulatory plug was noted up to the 28(th) day after delivery, were supplemented daily with lyophilized aqueous (RKW) or 50% hydro-ethanolic (RKW-A) extract (20 mg/kg b.m.) dissolved in distilled water. Then, mice were euthanized, spleens dissected, cells counted and the total numbers of CD3+, CD19+, CD4+, CD8+ and CD335+ splenic lymphocytes were evaluated by cytometry. The number of CD3+ lymphocytes per 1 g of splenic tissue was higher in RKW-A than in RKW spleens and did not differ from the control. The number of CD3+ lymphocytes in RKW spleens was lower than in the controls. The number of CD19+ and CD4+ cells was lower in both experimental groups than in the controls. The number of CD335+(NK) cells was lower in RKW spleens than in the control. Polish Society of Experimental and Clinical Immunology 2017-12-30 2017 /pmc/articles/PMC5820988/ /pubmed/29472809 http://dx.doi.org/10.5114/ceji.2017.72826 Text en Copyright: © 2017 Polish Society of Experimental and Clinical Immunology http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Experimental Immunology Lewicki, Sławomir Skopińska-Różewska, Ewa Zdanowski, Robert The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells |
title | The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells |
title_full | The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells |
title_fullStr | The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells |
title_full_unstemmed | The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells |
title_short | The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells |
title_sort | decrease in number of splenic lymphocytes in mice fed rhodiola kirilowii during pregnancy and lactation concerns mainly cd19+ and cd4+ cells |
topic | Experimental Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820988/ https://www.ncbi.nlm.nih.gov/pubmed/29472809 http://dx.doi.org/10.5114/ceji.2017.72826 |
work_keys_str_mv | AT lewickisławomir thedecreaseinnumberofspleniclymphocytesinmicefedrhodiolakirilowiiduringpregnancyandlactationconcernsmainlycd19andcd4cells AT skopinskarozewskaewa thedecreaseinnumberofspleniclymphocytesinmicefedrhodiolakirilowiiduringpregnancyandlactationconcernsmainlycd19andcd4cells AT zdanowskirobert thedecreaseinnumberofspleniclymphocytesinmicefedrhodiolakirilowiiduringpregnancyandlactationconcernsmainlycd19andcd4cells AT lewickisławomir decreaseinnumberofspleniclymphocytesinmicefedrhodiolakirilowiiduringpregnancyandlactationconcernsmainlycd19andcd4cells AT skopinskarozewskaewa decreaseinnumberofspleniclymphocytesinmicefedrhodiolakirilowiiduringpregnancyandlactationconcernsmainlycd19andcd4cells AT zdanowskirobert decreaseinnumberofspleniclymphocytesinmicefedrhodiolakirilowiiduringpregnancyandlactationconcernsmainlycd19andcd4cells |