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Distinguishing Human Peripheral Blood NK Cells from CD56(dim)CD16(dim)CD69(+)CD103(+) Resident Nasal Mucosal Lavage Fluid Cells

Natural killer (NK) cells are members of the innate lymphoid cells group 1 (ILC1s), which play a critical role in innate host defense against viruses and malignancies. While many studies have examined the role of circulating peripheral blood (PB) CD56(+) NK cells, little is known about the resident...

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Autores principales: Rebuli, Meghan E., Pawlak, Erica A., Walsh, Dana, Martin, Elizabeth M., Jaspers, Ilona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821812/
https://www.ncbi.nlm.nih.gov/pubmed/29467466
http://dx.doi.org/10.1038/s41598-018-21443-5
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author Rebuli, Meghan E.
Pawlak, Erica A.
Walsh, Dana
Martin, Elizabeth M.
Jaspers, Ilona
author_facet Rebuli, Meghan E.
Pawlak, Erica A.
Walsh, Dana
Martin, Elizabeth M.
Jaspers, Ilona
author_sort Rebuli, Meghan E.
collection PubMed
description Natural killer (NK) cells are members of the innate lymphoid cells group 1 (ILC1s), which play a critical role in innate host defense against viruses and malignancies. While many studies have examined the role of circulating peripheral blood (PB) CD56(+) NK cells, little is known about the resident CD56(+) cell population. Therefore, matched CD56(+) cells from nasal lavage fluid (NLF) and PB of smokers and non-smokers were compared phenotypically, via flow cytometry, and functionally, via NK-cell specific gene expression. NLF and PB CD56(+) cells had similar expression of CD56, but differentially expressed tissue residency (CD69 and CD103) and cytotoxicity (CD16) markers. In addition, NLF CD56(dim) cells expressed lower levels of cytotoxicity-associated genes, perforin (PRF1) and granzyme B (GZMB), and increased levels of cytokines and cell signaling molecules, TRAIL, IFNGR2, and IL8, as compared to PB CD56(dim) cells. In smokers, ITGA2 was downregulated in NLF CD56(dim) cells, while markers of cytotoxic function were primarily downregulated in PB CD56(dim) NK cells. Overall, NLF CD56(dim) cells are a unique cell population that likely play a role in orchestrating innate immune responses in the nasal cavity, which is distinct from their role as a non-antigen-restricted cytotoxic CD56(dim) lymphocytes in the PB.
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spelling pubmed-58218122018-02-26 Distinguishing Human Peripheral Blood NK Cells from CD56(dim)CD16(dim)CD69(+)CD103(+) Resident Nasal Mucosal Lavage Fluid Cells Rebuli, Meghan E. Pawlak, Erica A. Walsh, Dana Martin, Elizabeth M. Jaspers, Ilona Sci Rep Article Natural killer (NK) cells are members of the innate lymphoid cells group 1 (ILC1s), which play a critical role in innate host defense against viruses and malignancies. While many studies have examined the role of circulating peripheral blood (PB) CD56(+) NK cells, little is known about the resident CD56(+) cell population. Therefore, matched CD56(+) cells from nasal lavage fluid (NLF) and PB of smokers and non-smokers were compared phenotypically, via flow cytometry, and functionally, via NK-cell specific gene expression. NLF and PB CD56(+) cells had similar expression of CD56, but differentially expressed tissue residency (CD69 and CD103) and cytotoxicity (CD16) markers. In addition, NLF CD56(dim) cells expressed lower levels of cytotoxicity-associated genes, perforin (PRF1) and granzyme B (GZMB), and increased levels of cytokines and cell signaling molecules, TRAIL, IFNGR2, and IL8, as compared to PB CD56(dim) cells. In smokers, ITGA2 was downregulated in NLF CD56(dim) cells, while markers of cytotoxic function were primarily downregulated in PB CD56(dim) NK cells. Overall, NLF CD56(dim) cells are a unique cell population that likely play a role in orchestrating innate immune responses in the nasal cavity, which is distinct from their role as a non-antigen-restricted cytotoxic CD56(dim) lymphocytes in the PB. Nature Publishing Group UK 2018-02-21 /pmc/articles/PMC5821812/ /pubmed/29467466 http://dx.doi.org/10.1038/s41598-018-21443-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rebuli, Meghan E.
Pawlak, Erica A.
Walsh, Dana
Martin, Elizabeth M.
Jaspers, Ilona
Distinguishing Human Peripheral Blood NK Cells from CD56(dim)CD16(dim)CD69(+)CD103(+) Resident Nasal Mucosal Lavage Fluid Cells
title Distinguishing Human Peripheral Blood NK Cells from CD56(dim)CD16(dim)CD69(+)CD103(+) Resident Nasal Mucosal Lavage Fluid Cells
title_full Distinguishing Human Peripheral Blood NK Cells from CD56(dim)CD16(dim)CD69(+)CD103(+) Resident Nasal Mucosal Lavage Fluid Cells
title_fullStr Distinguishing Human Peripheral Blood NK Cells from CD56(dim)CD16(dim)CD69(+)CD103(+) Resident Nasal Mucosal Lavage Fluid Cells
title_full_unstemmed Distinguishing Human Peripheral Blood NK Cells from CD56(dim)CD16(dim)CD69(+)CD103(+) Resident Nasal Mucosal Lavage Fluid Cells
title_short Distinguishing Human Peripheral Blood NK Cells from CD56(dim)CD16(dim)CD69(+)CD103(+) Resident Nasal Mucosal Lavage Fluid Cells
title_sort distinguishing human peripheral blood nk cells from cd56(dim)cd16(dim)cd69(+)cd103(+) resident nasal mucosal lavage fluid cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821812/
https://www.ncbi.nlm.nih.gov/pubmed/29467466
http://dx.doi.org/10.1038/s41598-018-21443-5
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