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Transcriptome changes induced by abiotic stresses in Artemisia annua

Artemisia annua is known to be the source of artemisinin worldwide which is an antimalarial compound but is synthesised in very limited amount in the plant. Most research laid emphasis on the methods of enhancing artemisinin but our study has been planned in a way that it may simultaneously address...

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Autores principales: Vashisth, Divya, Kumar, Ritesh, Rastogi, Shubhra, Patel, Vikas Kumar, Kalra, Alok, Gupta, Madan Mohan, Gupta, Anil Kumar, Shasany, Ajit Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821844/
https://www.ncbi.nlm.nih.gov/pubmed/29467423
http://dx.doi.org/10.1038/s41598-018-21598-1
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author Vashisth, Divya
Kumar, Ritesh
Rastogi, Shubhra
Patel, Vikas Kumar
Kalra, Alok
Gupta, Madan Mohan
Gupta, Anil Kumar
Shasany, Ajit Kumar
author_facet Vashisth, Divya
Kumar, Ritesh
Rastogi, Shubhra
Patel, Vikas Kumar
Kalra, Alok
Gupta, Madan Mohan
Gupta, Anil Kumar
Shasany, Ajit Kumar
author_sort Vashisth, Divya
collection PubMed
description Artemisia annua is known to be the source of artemisinin worldwide which is an antimalarial compound but is synthesised in very limited amount in the plant. Most research laid emphasis on the methods of enhancing artemisinin but our study has been planned in a way that it may simultaneously address two problems encountered by the plant. Firstly, to know the effect on the artemisinin content in the era of climate change because the secondary metabolites tend to increase under stress. Secondly, to identify some of the stress responsive genes that could help in stress tolerance of the plant under abiotic stress. Hence, the A. annua plants were subjected to four abiotic stresses (salt, cold, drought and water-logging) and it was observed that the artemisinin content increased in all the stress conditions except drought. Next, in order to identify the stress responsive genes, the transcriptome sequencing of the plants under stress was carried out resulting in 89,362 transcripts for control and 81,328, 76,337, 90,470 and 96,493 transcripts for salt, cold, drought, and water logging stresses. This investigation provides new insights for functional studies of genes involved in multiple abiotic stresses and potential candidate genes for multiple stress tolerance in A. annua.
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spelling pubmed-58218442018-02-26 Transcriptome changes induced by abiotic stresses in Artemisia annua Vashisth, Divya Kumar, Ritesh Rastogi, Shubhra Patel, Vikas Kumar Kalra, Alok Gupta, Madan Mohan Gupta, Anil Kumar Shasany, Ajit Kumar Sci Rep Article Artemisia annua is known to be the source of artemisinin worldwide which is an antimalarial compound but is synthesised in very limited amount in the plant. Most research laid emphasis on the methods of enhancing artemisinin but our study has been planned in a way that it may simultaneously address two problems encountered by the plant. Firstly, to know the effect on the artemisinin content in the era of climate change because the secondary metabolites tend to increase under stress. Secondly, to identify some of the stress responsive genes that could help in stress tolerance of the plant under abiotic stress. Hence, the A. annua plants were subjected to four abiotic stresses (salt, cold, drought and water-logging) and it was observed that the artemisinin content increased in all the stress conditions except drought. Next, in order to identify the stress responsive genes, the transcriptome sequencing of the plants under stress was carried out resulting in 89,362 transcripts for control and 81,328, 76,337, 90,470 and 96,493 transcripts for salt, cold, drought, and water logging stresses. This investigation provides new insights for functional studies of genes involved in multiple abiotic stresses and potential candidate genes for multiple stress tolerance in A. annua. Nature Publishing Group UK 2018-02-21 /pmc/articles/PMC5821844/ /pubmed/29467423 http://dx.doi.org/10.1038/s41598-018-21598-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Vashisth, Divya
Kumar, Ritesh
Rastogi, Shubhra
Patel, Vikas Kumar
Kalra, Alok
Gupta, Madan Mohan
Gupta, Anil Kumar
Shasany, Ajit Kumar
Transcriptome changes induced by abiotic stresses in Artemisia annua
title Transcriptome changes induced by abiotic stresses in Artemisia annua
title_full Transcriptome changes induced by abiotic stresses in Artemisia annua
title_fullStr Transcriptome changes induced by abiotic stresses in Artemisia annua
title_full_unstemmed Transcriptome changes induced by abiotic stresses in Artemisia annua
title_short Transcriptome changes induced by abiotic stresses in Artemisia annua
title_sort transcriptome changes induced by abiotic stresses in artemisia annua
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821844/
https://www.ncbi.nlm.nih.gov/pubmed/29467423
http://dx.doi.org/10.1038/s41598-018-21598-1
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