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Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells
Nitric oxide (NO) is thought to play a pivotal regulatory role in dental pulp tissues under both physiological and pathological conditions. However, little is known about the NO functions in dental pulp stem cells (DPSCs). We examined the direct actions of a spontaneous NO gas-releasing donor, NOC-1...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821879/ https://www.ncbi.nlm.nih.gov/pubmed/29467418 http://dx.doi.org/10.1038/s41598-018-21183-6 |
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author | Sonoda, Soichiro Mei, Yu-feng Atsuta, Ikiru Danjo, Atsushi Yamaza, Haruyoshi Hama, Shion Nishida, Kento Tang, Ronghao Kyumoto-Nakamura, Yukari Uehara, Norihisa Kukita, Toshio Nishimura, Fusanori Yamaza, Takayoshi |
author_facet | Sonoda, Soichiro Mei, Yu-feng Atsuta, Ikiru Danjo, Atsushi Yamaza, Haruyoshi Hama, Shion Nishida, Kento Tang, Ronghao Kyumoto-Nakamura, Yukari Uehara, Norihisa Kukita, Toshio Nishimura, Fusanori Yamaza, Takayoshi |
author_sort | Sonoda, Soichiro |
collection | PubMed |
description | Nitric oxide (NO) is thought to play a pivotal regulatory role in dental pulp tissues under both physiological and pathological conditions. However, little is known about the NO functions in dental pulp stem cells (DPSCs). We examined the direct actions of a spontaneous NO gas-releasing donor, NOC-18, on the odontogenic capacity of rat DPSCs (rDPSCs). In the presence of NOC-18, rDPSCs were transformed into odontoblast-like cells with long cytoplasmic processes and a polarized nucleus. NOC-18 treatment increased alkaline phosphatase activity and enhanced dentin-like mineralized tissue formation and the expression levels of several odontoblast-specific genes, such as runt related factor 2, dentin matrix protein 1 and dentin sialophosphoprotein, in rDPSCs. In contrast, carboxy-PTIO, a NO scavenger, completely suppressed the odontogenic capacity of rDPSCs. This NO-promoted odontogenic differentiation was activated by tumor necrosis factor-NF-κB axis in rDPSCs. Further in vivo study demonstrated that NOC-18-application in a tooth cavity accelerated tertiary dentin formation, which was associated with early nitrotyrosine expression in the dental pulp tissues beneath the cavity. Taken together, the present findings indicate that exogenous NO directly induces the odontogenic capacity of rDPSCs, suggesting that NO donors might offer a novel host DPSC-targeting alternative to current pulp capping agents in endodontics. |
format | Online Article Text |
id | pubmed-5821879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58218792018-02-26 Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells Sonoda, Soichiro Mei, Yu-feng Atsuta, Ikiru Danjo, Atsushi Yamaza, Haruyoshi Hama, Shion Nishida, Kento Tang, Ronghao Kyumoto-Nakamura, Yukari Uehara, Norihisa Kukita, Toshio Nishimura, Fusanori Yamaza, Takayoshi Sci Rep Article Nitric oxide (NO) is thought to play a pivotal regulatory role in dental pulp tissues under both physiological and pathological conditions. However, little is known about the NO functions in dental pulp stem cells (DPSCs). We examined the direct actions of a spontaneous NO gas-releasing donor, NOC-18, on the odontogenic capacity of rat DPSCs (rDPSCs). In the presence of NOC-18, rDPSCs were transformed into odontoblast-like cells with long cytoplasmic processes and a polarized nucleus. NOC-18 treatment increased alkaline phosphatase activity and enhanced dentin-like mineralized tissue formation and the expression levels of several odontoblast-specific genes, such as runt related factor 2, dentin matrix protein 1 and dentin sialophosphoprotein, in rDPSCs. In contrast, carboxy-PTIO, a NO scavenger, completely suppressed the odontogenic capacity of rDPSCs. This NO-promoted odontogenic differentiation was activated by tumor necrosis factor-NF-κB axis in rDPSCs. Further in vivo study demonstrated that NOC-18-application in a tooth cavity accelerated tertiary dentin formation, which was associated with early nitrotyrosine expression in the dental pulp tissues beneath the cavity. Taken together, the present findings indicate that exogenous NO directly induces the odontogenic capacity of rDPSCs, suggesting that NO donors might offer a novel host DPSC-targeting alternative to current pulp capping agents in endodontics. Nature Publishing Group UK 2018-02-21 /pmc/articles/PMC5821879/ /pubmed/29467418 http://dx.doi.org/10.1038/s41598-018-21183-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Sonoda, Soichiro Mei, Yu-feng Atsuta, Ikiru Danjo, Atsushi Yamaza, Haruyoshi Hama, Shion Nishida, Kento Tang, Ronghao Kyumoto-Nakamura, Yukari Uehara, Norihisa Kukita, Toshio Nishimura, Fusanori Yamaza, Takayoshi Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells |
title | Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells |
title_full | Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells |
title_fullStr | Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells |
title_full_unstemmed | Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells |
title_short | Exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells |
title_sort | exogenous nitric oxide stimulates the odontogenic differentiation of rat dental pulp stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5821879/ https://www.ncbi.nlm.nih.gov/pubmed/29467418 http://dx.doi.org/10.1038/s41598-018-21183-6 |
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