Cargando…

Sweet attraction: sugarcane pollen-associated volatiles attract gravid Anopheles arabiensis

BACKGROUND: Anopheles arabiensis is a key vector for the transmission of human malaria in sub-Saharan Africa. Over the past 10,000 years, humans have successfully cultivated grasses and altered the landscape, creating An. arabiensis favourable environments that contain excellent habitats for both la...

Descripción completa

Detalles Bibliográficos
Autores principales: Wondwosen, Betelehem, Birgersson, Göran, Tekie, Habte, Torto, Baldwyn, Ignell, Rickard, Hill, Sharon R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822481/
https://www.ncbi.nlm.nih.gov/pubmed/29466989
http://dx.doi.org/10.1186/s12936-018-2245-1
_version_ 1783301692930916352
author Wondwosen, Betelehem
Birgersson, Göran
Tekie, Habte
Torto, Baldwyn
Ignell, Rickard
Hill, Sharon R.
author_facet Wondwosen, Betelehem
Birgersson, Göran
Tekie, Habte
Torto, Baldwyn
Ignell, Rickard
Hill, Sharon R.
author_sort Wondwosen, Betelehem
collection PubMed
description BACKGROUND: Anopheles arabiensis is a key vector for the transmission of human malaria in sub-Saharan Africa. Over the past 10,000 years, humans have successfully cultivated grasses and altered the landscape, creating An. arabiensis favourable environments that contain excellent habitats for both larvae and adults. Sugarcane is the most expanding agricultural system in sub-Saharan Africa, and is linked to the increased threat of malaria in rural communities. The prolific production and wind dispersal of sugarcane pollen, together with standing pools of water, often provide, as a result of irrigation, a nutrient-rich environment for the offspring of gravid malaria mosquitoes. RESULTS: In the present study, sugarcane pollen-associated volatiles from two cultivars are shown to attract gravid An. arabiensis in a still air two-port olfactometer and stimulate egg laying in an oviposition bioassay. Through combined gas chromatography and electroantennographic detection, as well as combined gas chromatography and mass spectrometric analyses, we identified the bioactive volatiles and generated a synthetic blend that reproduced the full behavioural repertoire of gravid mosquitoes in the Y-tube assay. Two subtractive odour blends, when compared with the full blend, were significantly more attractive. These three and four-component subtractive blends share the compounds (1R)-(+)-α-pinene, nonanal and benzaldehyde, of which, (1R)-(+)-α-pinene and nonanal are found in the attractive odour blends from rice plants and maize pollen. In pairwise comparisons, the rice synthetic odour blend was more attractive to gravid mosquitoes than either of the pollen blends, whereas the pollen blends did not differ in attraction. CONCLUSIONS: The attraction of gravid females to sugarcane pollen volatiles demonstrated in this study, together with the previously found grass-associated volatiles, raise the potential of developing a bioactive chimeric blend to attract gravid malaria mosquitoes. This is discussed in relation to the development of novel and cost-effective vector control measures.
format Online
Article
Text
id pubmed-5822481
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-58224812018-02-26 Sweet attraction: sugarcane pollen-associated volatiles attract gravid Anopheles arabiensis Wondwosen, Betelehem Birgersson, Göran Tekie, Habte Torto, Baldwyn Ignell, Rickard Hill, Sharon R. Malar J Research BACKGROUND: Anopheles arabiensis is a key vector for the transmission of human malaria in sub-Saharan Africa. Over the past 10,000 years, humans have successfully cultivated grasses and altered the landscape, creating An. arabiensis favourable environments that contain excellent habitats for both larvae and adults. Sugarcane is the most expanding agricultural system in sub-Saharan Africa, and is linked to the increased threat of malaria in rural communities. The prolific production and wind dispersal of sugarcane pollen, together with standing pools of water, often provide, as a result of irrigation, a nutrient-rich environment for the offspring of gravid malaria mosquitoes. RESULTS: In the present study, sugarcane pollen-associated volatiles from two cultivars are shown to attract gravid An. arabiensis in a still air two-port olfactometer and stimulate egg laying in an oviposition bioassay. Through combined gas chromatography and electroantennographic detection, as well as combined gas chromatography and mass spectrometric analyses, we identified the bioactive volatiles and generated a synthetic blend that reproduced the full behavioural repertoire of gravid mosquitoes in the Y-tube assay. Two subtractive odour blends, when compared with the full blend, were significantly more attractive. These three and four-component subtractive blends share the compounds (1R)-(+)-α-pinene, nonanal and benzaldehyde, of which, (1R)-(+)-α-pinene and nonanal are found in the attractive odour blends from rice plants and maize pollen. In pairwise comparisons, the rice synthetic odour blend was more attractive to gravid mosquitoes than either of the pollen blends, whereas the pollen blends did not differ in attraction. CONCLUSIONS: The attraction of gravid females to sugarcane pollen volatiles demonstrated in this study, together with the previously found grass-associated volatiles, raise the potential of developing a bioactive chimeric blend to attract gravid malaria mosquitoes. This is discussed in relation to the development of novel and cost-effective vector control measures. BioMed Central 2018-02-21 /pmc/articles/PMC5822481/ /pubmed/29466989 http://dx.doi.org/10.1186/s12936-018-2245-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wondwosen, Betelehem
Birgersson, Göran
Tekie, Habte
Torto, Baldwyn
Ignell, Rickard
Hill, Sharon R.
Sweet attraction: sugarcane pollen-associated volatiles attract gravid Anopheles arabiensis
title Sweet attraction: sugarcane pollen-associated volatiles attract gravid Anopheles arabiensis
title_full Sweet attraction: sugarcane pollen-associated volatiles attract gravid Anopheles arabiensis
title_fullStr Sweet attraction: sugarcane pollen-associated volatiles attract gravid Anopheles arabiensis
title_full_unstemmed Sweet attraction: sugarcane pollen-associated volatiles attract gravid Anopheles arabiensis
title_short Sweet attraction: sugarcane pollen-associated volatiles attract gravid Anopheles arabiensis
title_sort sweet attraction: sugarcane pollen-associated volatiles attract gravid anopheles arabiensis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822481/
https://www.ncbi.nlm.nih.gov/pubmed/29466989
http://dx.doi.org/10.1186/s12936-018-2245-1
work_keys_str_mv AT wondwosenbetelehem sweetattractionsugarcanepollenassociatedvolatilesattractgravidanophelesarabiensis
AT birgerssongoran sweetattractionsugarcanepollenassociatedvolatilesattractgravidanophelesarabiensis
AT tekiehabte sweetattractionsugarcanepollenassociatedvolatilesattractgravidanophelesarabiensis
AT tortobaldwyn sweetattractionsugarcanepollenassociatedvolatilesattractgravidanophelesarabiensis
AT ignellrickard sweetattractionsugarcanepollenassociatedvolatilesattractgravidanophelesarabiensis
AT hillsharonr sweetattractionsugarcanepollenassociatedvolatilesattractgravidanophelesarabiensis