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Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children

BACKGROUND: Lactobacillus rhamnosus Pen is a human endogenous strain with well-documented health promoting properties that is used for production of probiotics. It has a long safety history of application, and its effectiveness in the prevention of antibiotic-associated diarrhoea has also been confi...

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Autores principales: Jarocki, Piotr, Podleśny, Marcin, Krawczyk, Mariusz, Glibowska, Agnieszka, Pawelec, Jarosław, Komoń-Janczara, Elwira, Kholiavskyi, Oleksandr, Dworniczak, Michał, Targoński, Zdzisław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822663/
https://www.ncbi.nlm.nih.gov/pubmed/29483943
http://dx.doi.org/10.1186/s13099-018-0235-z
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author Jarocki, Piotr
Podleśny, Marcin
Krawczyk, Mariusz
Glibowska, Agnieszka
Pawelec, Jarosław
Komoń-Janczara, Elwira
Kholiavskyi, Oleksandr
Dworniczak, Michał
Targoński, Zdzisław
author_facet Jarocki, Piotr
Podleśny, Marcin
Krawczyk, Mariusz
Glibowska, Agnieszka
Pawelec, Jarosław
Komoń-Janczara, Elwira
Kholiavskyi, Oleksandr
Dworniczak, Michał
Targoński, Zdzisław
author_sort Jarocki, Piotr
collection PubMed
description BACKGROUND: Lactobacillus rhamnosus Pen is a human endogenous strain with well-documented health promoting properties that is used for production of probiotics. It has a long safety history of application, and its effectiveness in the prevention of antibiotic-associated diarrhoea has also been confirmed in clinical trials. RESULTS: Here we present the complete genome sequence of L. rhamnosus Pen, which consists of a circular 2,884,4966-bp chromosome with a GC content of 46.8%. Within 2907 open reading frames (ORFs), genes involved with probiotic properties were identified. A CRISPR locus, consisting of a 1092-nt region with 16 spacers, was also detected. Finally, an intact prophage of ~ 40.7 kb, 57 ORFs, GC content 44.8% was identified. CONCLUSIONS: Genomic analysis confirmed the probiotic properties of L. rhamnosus Pen and may indicate new biotechnological applications of this industrially important strain. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0235-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-58226632018-02-26 Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children Jarocki, Piotr Podleśny, Marcin Krawczyk, Mariusz Glibowska, Agnieszka Pawelec, Jarosław Komoń-Janczara, Elwira Kholiavskyi, Oleksandr Dworniczak, Michał Targoński, Zdzisław Gut Pathog Genome Report BACKGROUND: Lactobacillus rhamnosus Pen is a human endogenous strain with well-documented health promoting properties that is used for production of probiotics. It has a long safety history of application, and its effectiveness in the prevention of antibiotic-associated diarrhoea has also been confirmed in clinical trials. RESULTS: Here we present the complete genome sequence of L. rhamnosus Pen, which consists of a circular 2,884,4966-bp chromosome with a GC content of 46.8%. Within 2907 open reading frames (ORFs), genes involved with probiotic properties were identified. A CRISPR locus, consisting of a 1092-nt region with 16 spacers, was also detected. Finally, an intact prophage of ~ 40.7 kb, 57 ORFs, GC content 44.8% was identified. CONCLUSIONS: Genomic analysis confirmed the probiotic properties of L. rhamnosus Pen and may indicate new biotechnological applications of this industrially important strain. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0235-z) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-22 /pmc/articles/PMC5822663/ /pubmed/29483943 http://dx.doi.org/10.1186/s13099-018-0235-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Genome Report
Jarocki, Piotr
Podleśny, Marcin
Krawczyk, Mariusz
Glibowska, Agnieszka
Pawelec, Jarosław
Komoń-Janczara, Elwira
Kholiavskyi, Oleksandr
Dworniczak, Michał
Targoński, Zdzisław
Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
title Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
title_full Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
title_fullStr Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
title_full_unstemmed Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
title_short Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
title_sort complete genome sequence of lactobacillus rhamnosus pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
topic Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822663/
https://www.ncbi.nlm.nih.gov/pubmed/29483943
http://dx.doi.org/10.1186/s13099-018-0235-z
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