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Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival
Patients with glioblastoma multiforme (GBM) are at high risk to develop a relapse despite multimodal therapy. Assumedly, glioma stem cells (GSCs) are responsible for treatment resistance of GBM. Identification of specific GSC markers may help to develop targeted therapies. Here, we performed express...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822829/ https://www.ncbi.nlm.nih.gov/pubmed/29535786 http://dx.doi.org/10.1155/2018/9628289 |
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author | Bien-Möller, Sandra Balz, Ellen Herzog, Susann Plantera, Laura Vogelgesang, Silke Weitmann, Kerstin Seifert, Carolin Fink, Matthias A. Marx, Sascha Bialke, Angela Venugopal, Chitra Singh, Sheila K. Hoffmann, Wolfgang Rauch, Bernhard H. Schroeder, Henry W. S. |
author_facet | Bien-Möller, Sandra Balz, Ellen Herzog, Susann Plantera, Laura Vogelgesang, Silke Weitmann, Kerstin Seifert, Carolin Fink, Matthias A. Marx, Sascha Bialke, Angela Venugopal, Chitra Singh, Sheila K. Hoffmann, Wolfgang Rauch, Bernhard H. Schroeder, Henry W. S. |
author_sort | Bien-Möller, Sandra |
collection | PubMed |
description | Patients with glioblastoma multiforme (GBM) are at high risk to develop a relapse despite multimodal therapy. Assumedly, glioma stem cells (GSCs) are responsible for treatment resistance of GBM. Identification of specific GSC markers may help to develop targeted therapies. Here, we performed expression analyses of stem cell (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) as well as differentiation and microglia markers (GFAP, Iba1, and Sparc) in GBM compared to nonmalignant brain. Furthermore, the role of these proteins for patient survival and their expression in LN18 stem-like neurospheres was analyzed. At mRNA level, ABCG2 and CD95 were reduced, GFAP was unchanged; all other investigated markers were increased in GBM. At protein level, CD44, ELF4, Nanog, Nestin, and Sparc were elevated in GBM, but only CD133 and Nestin were strongly associated with survival time. In addition, ABCG2 and GFAP expression was decreased in LN18 neurospheres whereas CD44, CD95, CD133, ELF4, Nanog, Nestin, and Sparc were upregulated. Altogether only CD133 and Nestin were associated with survival rates. This raises concerns regarding the suitability of the other target structures as prognostic markers, but makes both CD133 and Nestin candidates for GBM therapy. Nevertheless, a search for more specific marker proteins is urgently needed. |
format | Online Article Text |
id | pubmed-5822829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-58228292018-03-13 Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival Bien-Möller, Sandra Balz, Ellen Herzog, Susann Plantera, Laura Vogelgesang, Silke Weitmann, Kerstin Seifert, Carolin Fink, Matthias A. Marx, Sascha Bialke, Angela Venugopal, Chitra Singh, Sheila K. Hoffmann, Wolfgang Rauch, Bernhard H. Schroeder, Henry W. S. Stem Cells Int Research Article Patients with glioblastoma multiforme (GBM) are at high risk to develop a relapse despite multimodal therapy. Assumedly, glioma stem cells (GSCs) are responsible for treatment resistance of GBM. Identification of specific GSC markers may help to develop targeted therapies. Here, we performed expression analyses of stem cell (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) as well as differentiation and microglia markers (GFAP, Iba1, and Sparc) in GBM compared to nonmalignant brain. Furthermore, the role of these proteins for patient survival and their expression in LN18 stem-like neurospheres was analyzed. At mRNA level, ABCG2 and CD95 were reduced, GFAP was unchanged; all other investigated markers were increased in GBM. At protein level, CD44, ELF4, Nanog, Nestin, and Sparc were elevated in GBM, but only CD133 and Nestin were strongly associated with survival time. In addition, ABCG2 and GFAP expression was decreased in LN18 neurospheres whereas CD44, CD95, CD133, ELF4, Nanog, Nestin, and Sparc were upregulated. Altogether only CD133 and Nestin were associated with survival rates. This raises concerns regarding the suitability of the other target structures as prognostic markers, but makes both CD133 and Nestin candidates for GBM therapy. Nevertheless, a search for more specific marker proteins is urgently needed. Hindawi 2018-01-17 /pmc/articles/PMC5822829/ /pubmed/29535786 http://dx.doi.org/10.1155/2018/9628289 Text en Copyright © 2018 Sandra Bien-Möller et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bien-Möller, Sandra Balz, Ellen Herzog, Susann Plantera, Laura Vogelgesang, Silke Weitmann, Kerstin Seifert, Carolin Fink, Matthias A. Marx, Sascha Bialke, Angela Venugopal, Chitra Singh, Sheila K. Hoffmann, Wolfgang Rauch, Bernhard H. Schroeder, Henry W. S. Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival |
title | Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival |
title_full | Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival |
title_fullStr | Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival |
title_full_unstemmed | Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival |
title_short | Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival |
title_sort | association of glioblastoma multiforme stem cell characteristics, differentiation, and microglia marker genes with patient survival |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822829/ https://www.ncbi.nlm.nih.gov/pubmed/29535786 http://dx.doi.org/10.1155/2018/9628289 |
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