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Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation

Arctium lappa (A. lappa), Compositae, is considered a potential source of nutrition and is used as a traditional medicine in East Asian countries for centuries. Although several studies have shown its biological activities as an anti-inflammatory agent, there have been no reports on A. lappa with re...

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Autores principales: Kim, Young-Kyu, Koppula, Sushruta, Shim, Do-Wan, In, Eun-Jung, Kwak, Su-Bin, Kim, Myong-Ki, Yu, Sang-Hyeun, Lee, Kwang-Ho, Kang, Tae-Bong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822850/
https://www.ncbi.nlm.nih.gov/pubmed/29576797
http://dx.doi.org/10.1155/2018/6346734
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author Kim, Young-Kyu
Koppula, Sushruta
Shim, Do-Wan
In, Eun-Jung
Kwak, Su-Bin
Kim, Myong-Ki
Yu, Sang-Hyeun
Lee, Kwang-Ho
Kang, Tae-Bong
author_facet Kim, Young-Kyu
Koppula, Sushruta
Shim, Do-Wan
In, Eun-Jung
Kwak, Su-Bin
Kim, Myong-Ki
Yu, Sang-Hyeun
Lee, Kwang-Ho
Kang, Tae-Bong
author_sort Kim, Young-Kyu
collection PubMed
description Arctium lappa (A. lappa), Compositae, is considered a potential source of nutrition and is used as a traditional medicine in East Asian countries for centuries. Although several studies have shown its biological activities as an anti-inflammatory agent, there have been no reports on A. lappa with regard to regulatory role in inflammasome activation. The purpose of this study was to investigate the inhibitory effects of A. lappa extract (ALE) on NLRP3 inflammasome activation and explore the underlying mechanisms. We found that ALE inhibited IL-1β secretion from NLRP3 inflammasome activated bone marrow derived macrophages but not that secreted by NLRC4 and AIM2 inflammasomes activation. Mechanistic studies revealed that ALE suppressed the ATPase activity of purified NLRP3 and reduced mitochondrial reactive oxygen species (mROS) generated during NLRP3 activation. Therefore, the inhibitory effect of ALE on NLRP3 inflammasome might be attributed to its ability to inhibit the NLRP3 ATPase function and attenuated the mROS during inflammasome activation. In addition, ALE significantly reduced the LPS-induced increase of plasma IL-1β in mouse peritonitis model. These results provide evidence of novel anti-inflammatory mechanisms of A. lappa, which might be used for therapeutic applications in the treatment of NLRP3 inflammasome-associated inflammatory disorders.
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spelling pubmed-58228502018-03-25 Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation Kim, Young-Kyu Koppula, Sushruta Shim, Do-Wan In, Eun-Jung Kwak, Su-Bin Kim, Myong-Ki Yu, Sang-Hyeun Lee, Kwang-Ho Kang, Tae-Bong Evid Based Complement Alternat Med Research Article Arctium lappa (A. lappa), Compositae, is considered a potential source of nutrition and is used as a traditional medicine in East Asian countries for centuries. Although several studies have shown its biological activities as an anti-inflammatory agent, there have been no reports on A. lappa with regard to regulatory role in inflammasome activation. The purpose of this study was to investigate the inhibitory effects of A. lappa extract (ALE) on NLRP3 inflammasome activation and explore the underlying mechanisms. We found that ALE inhibited IL-1β secretion from NLRP3 inflammasome activated bone marrow derived macrophages but not that secreted by NLRC4 and AIM2 inflammasomes activation. Mechanistic studies revealed that ALE suppressed the ATPase activity of purified NLRP3 and reduced mitochondrial reactive oxygen species (mROS) generated during NLRP3 activation. Therefore, the inhibitory effect of ALE on NLRP3 inflammasome might be attributed to its ability to inhibit the NLRP3 ATPase function and attenuated the mROS during inflammasome activation. In addition, ALE significantly reduced the LPS-induced increase of plasma IL-1β in mouse peritonitis model. These results provide evidence of novel anti-inflammatory mechanisms of A. lappa, which might be used for therapeutic applications in the treatment of NLRP3 inflammasome-associated inflammatory disorders. Hindawi 2018-01-18 /pmc/articles/PMC5822850/ /pubmed/29576797 http://dx.doi.org/10.1155/2018/6346734 Text en Copyright © 2018 Young-Kyu Kim et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Young-Kyu
Koppula, Sushruta
Shim, Do-Wan
In, Eun-Jung
Kwak, Su-Bin
Kim, Myong-Ki
Yu, Sang-Hyeun
Lee, Kwang-Ho
Kang, Tae-Bong
Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation
title Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation
title_full Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation
title_fullStr Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation
title_full_unstemmed Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation
title_short Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation
title_sort inhibitory effect and mechanism of arctium lappa extract on nlrp3 inflammasome activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822850/
https://www.ncbi.nlm.nih.gov/pubmed/29576797
http://dx.doi.org/10.1155/2018/6346734
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