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Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism

AIM: The study was conducted to investigate the antibacterial and antiasthmatic effects of Nandina domestica leaf extract, to find out its active components, and to assess its safety issue. METHODS: (1) Solid-phase agar dilution method was used for antibacterial activity test of nandina leaf extract...

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Autores principales: Guo, Zhao-Yu, Zhang, Zhuo-Yang, Xiao, Jia-Qi, Qin, Jin-Hong, Zhao, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822912/
https://www.ncbi.nlm.nih.gov/pubmed/29576801
http://dx.doi.org/10.1155/2018/8298151
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author Guo, Zhao-Yu
Zhang, Zhuo-Yang
Xiao, Jia-Qi
Qin, Jin-Hong
Zhao, Wei
author_facet Guo, Zhao-Yu
Zhang, Zhuo-Yang
Xiao, Jia-Qi
Qin, Jin-Hong
Zhao, Wei
author_sort Guo, Zhao-Yu
collection PubMed
description AIM: The study was conducted to investigate the antibacterial and antiasthmatic effects of Nandina domestica leaf extract, to find out its active components, and to assess its safety issue. METHODS: (1) Solid-phase agar dilution method was used for antibacterial activity test of nandina leaf extract and the change of bacterial morphology after treatment was observed under the transmission microscope; (2) guinea pig model of asthma was used to test the asthma prevention effect of nandina leaf extract; (3) alkaloids and flavones were separated from nandina leaf extract and were further analyzed with HPLC-MS; (4) mice model was used to assessment of the safety issue of nandina leaf extract. RESULTS: (1) Nandina leaf extract inhibited the growth of bacteria and destroyed bacterial membrane; (2) nandina leaf extract alleviated animal allergy and asthma; (3) the components reextracted by ethyl acetate were active, in which alkaloids inhibited Gram-positive bacteria and prevented asthma and flavones inhibited Gram-negative bacteria; (4) nandina leaf extract had no toxic effect on mice. CONCLUSION: Nandina leaves inhibit bacterial growth and prevent asthma through alkaloids and flavones, which had integrated function against chronic bronchitis. This study provided theoretical basement for producing new Chinese medicine against chronic bronchitis.
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spelling pubmed-58229122018-03-25 Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism Guo, Zhao-Yu Zhang, Zhuo-Yang Xiao, Jia-Qi Qin, Jin-Hong Zhao, Wei Evid Based Complement Alternat Med Research Article AIM: The study was conducted to investigate the antibacterial and antiasthmatic effects of Nandina domestica leaf extract, to find out its active components, and to assess its safety issue. METHODS: (1) Solid-phase agar dilution method was used for antibacterial activity test of nandina leaf extract and the change of bacterial morphology after treatment was observed under the transmission microscope; (2) guinea pig model of asthma was used to test the asthma prevention effect of nandina leaf extract; (3) alkaloids and flavones were separated from nandina leaf extract and were further analyzed with HPLC-MS; (4) mice model was used to assessment of the safety issue of nandina leaf extract. RESULTS: (1) Nandina leaf extract inhibited the growth of bacteria and destroyed bacterial membrane; (2) nandina leaf extract alleviated animal allergy and asthma; (3) the components reextracted by ethyl acetate were active, in which alkaloids inhibited Gram-positive bacteria and prevented asthma and flavones inhibited Gram-negative bacteria; (4) nandina leaf extract had no toxic effect on mice. CONCLUSION: Nandina leaves inhibit bacterial growth and prevent asthma through alkaloids and flavones, which had integrated function against chronic bronchitis. This study provided theoretical basement for producing new Chinese medicine against chronic bronchitis. Hindawi 2018-01-18 /pmc/articles/PMC5822912/ /pubmed/29576801 http://dx.doi.org/10.1155/2018/8298151 Text en Copyright © 2018 Zhao-Yu Guo et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guo, Zhao-Yu
Zhang, Zhuo-Yang
Xiao, Jia-Qi
Qin, Jin-Hong
Zhao, Wei
Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism
title Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism
title_full Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism
title_fullStr Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism
title_full_unstemmed Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism
title_short Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism
title_sort antibacterial effects of leaf extract of nandina domestica and the underlined mechanism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822912/
https://www.ncbi.nlm.nih.gov/pubmed/29576801
http://dx.doi.org/10.1155/2018/8298151
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