Experimental and histopathological observation scoring methods for evaluation of wound healing properties of Jatyadi Ghrita

INTRODUCTION: Jatyadi ghrita is a classical Ayurvedic formulation indicated in the treatment of various types of ulcers. AIM: The study was designed to explore the wound healing properties of Jatyadi Ghrita in diabetes - induced rats. MATERIALS AND METHODS: In the present study, diabetes mellitus was induced to 6 to 8-week-old male Wistar rats by injecting streptozotocin cut 65 mg/kg body weight intravenously by 15 min prior to the administration of Nicotinamide at 230 mg/kg body weight intraperitoneally. Animals having diabetes were used for grouping namely, diabetic control (DC), Ghrita control (GC), positive control (PC), i.e., mupirocin HCl, Jatyadi Ghrita treatment and one group of non-DC. Full-thickness excision wound was created and diameter was recorded. Daily clinical observations were recorded. A wound scoring method was developed. Wound diameter and score were recorded on days 1, 2, 3, 5, 7, 9, 12, 14 and 15. Photographs were taken at the same time interval points. Body weight and feed consumption were recorded weekly. Animals were sacrificed at regular intervals to collect the wound area tissue for histopathology analysis. Obtained data was analyzed statistically. RESULTS AND OBSERVATION: It was observed that there was no significant difference in diameter and percent change in wound healing as compared to any control. However, clinical score and histopathological changes in Jatyadi Ghrita group were improved from the second day of the study as compared to control. CONCLUSION: This indicates that the drug has similar wound healing activity as compared to the modern drug mupirocin HCl.