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Localization of Liv2 as an Immature Hepatocyte Marker in EB Outgrowth
The objective of this study was to establish Liv2, a surface marker of mouse immature hepatocytes (hepatoblasts), as a selection tool for embryonic stem (ES) cell–derived immature hepatocytes by acquiring basic data on Liv2 in normal mouse embryos and by confirming Liv2 expression in mouse ES-derive...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
TheScientificWorldJOURNAL
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823152/ https://www.ncbi.nlm.nih.gov/pubmed/19252758 http://dx.doi.org/10.1100/tsw.2009.18 |
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author | Takashimizu, Ikkei Tanaka, Yoshiki Yoshie, Susumu Kano, Yoshiya Ichikawa, Hinako Cui, Li Ogiwara, Naoko Johkura, Kohei Sasaki, Katsunori |
author_facet | Takashimizu, Ikkei Tanaka, Yoshiki Yoshie, Susumu Kano, Yoshiya Ichikawa, Hinako Cui, Li Ogiwara, Naoko Johkura, Kohei Sasaki, Katsunori |
author_sort | Takashimizu, Ikkei |
collection | PubMed |
description | The objective of this study was to establish Liv2, a surface marker of mouse immature hepatocytes (hepatoblasts), as a selection tool for embryonic stem (ES) cell–derived immature hepatocytes by acquiring basic data on Liv2 in normal mouse embryos and by confirming Liv2 expression in mouse ES-derived cells. The estimated molecular weight of Liv2 was 40–45 kDa, and immunoreactivity was definitively detected in the cell membrane of fetal hepatocytes on embryonic day (E) 9.5, declined gradually until E12.5, and subsequently became undetectable. Liv2 was localized on and close to the cell membrane. Embryoid bodies (EB) were formed from mouse ES cells whose undifferentiated state was confirmed with immunostaining of Nanog by the hanging drop method. A few Liv2-positive cells occurred as a cluster in EB outgrowth on day 7, but only some of these were albumin (ALB)-positive on day 13. These cells had the same pattern of immunoreactivity, i.e., localization on the cell membrane, as immature hepatocytes in the developing liver, although there were other types of cells with a different pattern of immunoreactivity that were seen only as a granular pattern in the cytoplasm and without ALB or the neuronal marker nestin. These results suggest that Liv2 may be useful as a surface marker for immature hepatocytes derived from ES cells. This application would allow for the sole selection of immature hepatocytes and provide a useful tool for regenerative medicine. |
format | Online Article Text |
id | pubmed-5823152 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | TheScientificWorldJOURNAL |
record_format | MEDLINE/PubMed |
spelling | pubmed-58231522018-03-14 Localization of Liv2 as an Immature Hepatocyte Marker in EB Outgrowth Takashimizu, Ikkei Tanaka, Yoshiki Yoshie, Susumu Kano, Yoshiya Ichikawa, Hinako Cui, Li Ogiwara, Naoko Johkura, Kohei Sasaki, Katsunori ScientificWorldJournal Research Article The objective of this study was to establish Liv2, a surface marker of mouse immature hepatocytes (hepatoblasts), as a selection tool for embryonic stem (ES) cell–derived immature hepatocytes by acquiring basic data on Liv2 in normal mouse embryos and by confirming Liv2 expression in mouse ES-derived cells. The estimated molecular weight of Liv2 was 40–45 kDa, and immunoreactivity was definitively detected in the cell membrane of fetal hepatocytes on embryonic day (E) 9.5, declined gradually until E12.5, and subsequently became undetectable. Liv2 was localized on and close to the cell membrane. Embryoid bodies (EB) were formed from mouse ES cells whose undifferentiated state was confirmed with immunostaining of Nanog by the hanging drop method. A few Liv2-positive cells occurred as a cluster in EB outgrowth on day 7, but only some of these were albumin (ALB)-positive on day 13. These cells had the same pattern of immunoreactivity, i.e., localization on the cell membrane, as immature hepatocytes in the developing liver, although there were other types of cells with a different pattern of immunoreactivity that were seen only as a granular pattern in the cytoplasm and without ALB or the neuronal marker nestin. These results suggest that Liv2 may be useful as a surface marker for immature hepatocytes derived from ES cells. This application would allow for the sole selection of immature hepatocytes and provide a useful tool for regenerative medicine. TheScientificWorldJOURNAL 2009-03-01 /pmc/articles/PMC5823152/ /pubmed/19252758 http://dx.doi.org/10.1100/tsw.2009.18 Text en Copyright © 2009 Ikkei Takashimizu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Takashimizu, Ikkei Tanaka, Yoshiki Yoshie, Susumu Kano, Yoshiya Ichikawa, Hinako Cui, Li Ogiwara, Naoko Johkura, Kohei Sasaki, Katsunori Localization of Liv2 as an Immature Hepatocyte Marker in EB Outgrowth |
title | Localization of Liv2 as an Immature Hepatocyte Marker in EB Outgrowth |
title_full | Localization of Liv2 as an Immature Hepatocyte Marker in EB Outgrowth |
title_fullStr | Localization of Liv2 as an Immature Hepatocyte Marker in EB Outgrowth |
title_full_unstemmed | Localization of Liv2 as an Immature Hepatocyte Marker in EB Outgrowth |
title_short | Localization of Liv2 as an Immature Hepatocyte Marker in EB Outgrowth |
title_sort | localization of liv2 as an immature hepatocyte marker in eb outgrowth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823152/ https://www.ncbi.nlm.nih.gov/pubmed/19252758 http://dx.doi.org/10.1100/tsw.2009.18 |
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