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Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress
Differential scanning calorimetry (DSC) was applied to estimate the impact of the toxic oxidant potassium permanganate (PM) on the intracellular structural and functional alterations at whole cell level using soil bacteria Arthrobacter oxydans as a model culture. Differential scanning calorimetry (D...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
TheScientificWorldJOURNAL
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823177/ https://www.ncbi.nlm.nih.gov/pubmed/19219375 http://dx.doi.org/10.1100/tsw.2009.3 |
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author | Abuladze, Marina K. Sokhadze, Victor M. Namchevadze, Emma N. Kiziria, E. Tabatadze, Leila V. Lejava, Lia V. Gogichaishvili, Sh. Bakradze, Nugzar B. |
author_facet | Abuladze, Marina K. Sokhadze, Victor M. Namchevadze, Emma N. Kiziria, E. Tabatadze, Leila V. Lejava, Lia V. Gogichaishvili, Sh. Bakradze, Nugzar B. |
author_sort | Abuladze, Marina K. |
collection | PubMed |
description | Differential scanning calorimetry (DSC) was applied to estimate the impact of the toxic oxidant potassium permanganate (PM) on the intracellular structural and functional alterations at whole cell level using soil bacteria Arthrobacter oxydans as a model culture. Differential scanning calorimetry (DSC) was applied in order to estimate the impact of the toxic oxidant potassium permanganate (PM) on the intracellular structural and functional alterations at the whole cell level using the soil bacteria Arthrobacter oxydans as a model culture. We compared the total melting heat and the temperature of DNA-protein complex (DNP) melting at the PM application prior to the calorimetry measurement and after 24-h exposure at the concentration range 0.02–1.4 mM. The initial oxidative effect caused changes in the pattern of the whole cell melting spectra (mainly at the temperature range 56–78°C), the decrease of T(max) °C DNP melting, and did not influence significantly the total heat of bacterial melting at different concentrations of PM. The prolonged effect of permanganate up to 24 h was characterized by a biphasic dose-dependent response to stress estimated by the DSC technique and the colony-forming assay. The low doses of PM (0.02 and 0.2 mM) stimulated cell proliferation, and increased the total whole cell melting heat and the temperature of DNP melting. The toxic effect of PM up to 0.04 mM reduced cell viability, changed the character of multipeaked thermograms, and lowered the total melting heat and the temperature of DNP melting in a concentration-dependent manner. This study presents the DSC method for evaluating and monitoring the effects of exposure to potential human and environmental toxicants. |
format | Online Article Text |
id | pubmed-5823177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | TheScientificWorldJOURNAL |
record_format | MEDLINE/PubMed |
spelling | pubmed-58231772018-03-14 Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress Abuladze, Marina K. Sokhadze, Victor M. Namchevadze, Emma N. Kiziria, E. Tabatadze, Leila V. Lejava, Lia V. Gogichaishvili, Sh. Bakradze, Nugzar B. ScientificWorldJournal Research Article Differential scanning calorimetry (DSC) was applied to estimate the impact of the toxic oxidant potassium permanganate (PM) on the intracellular structural and functional alterations at whole cell level using soil bacteria Arthrobacter oxydans as a model culture. Differential scanning calorimetry (DSC) was applied in order to estimate the impact of the toxic oxidant potassium permanganate (PM) on the intracellular structural and functional alterations at the whole cell level using the soil bacteria Arthrobacter oxydans as a model culture. We compared the total melting heat and the temperature of DNA-protein complex (DNP) melting at the PM application prior to the calorimetry measurement and after 24-h exposure at the concentration range 0.02–1.4 mM. The initial oxidative effect caused changes in the pattern of the whole cell melting spectra (mainly at the temperature range 56–78°C), the decrease of T(max) °C DNP melting, and did not influence significantly the total heat of bacterial melting at different concentrations of PM. The prolonged effect of permanganate up to 24 h was characterized by a biphasic dose-dependent response to stress estimated by the DSC technique and the colony-forming assay. The low doses of PM (0.02 and 0.2 mM) stimulated cell proliferation, and increased the total whole cell melting heat and the temperature of DNP melting. The toxic effect of PM up to 0.04 mM reduced cell viability, changed the character of multipeaked thermograms, and lowered the total melting heat and the temperature of DNP melting in a concentration-dependent manner. This study presents the DSC method for evaluating and monitoring the effects of exposure to potential human and environmental toxicants. TheScientificWorldJOURNAL 2009-02-15 /pmc/articles/PMC5823177/ /pubmed/19219375 http://dx.doi.org/10.1100/tsw.2009.3 Text en Copyright © 2009 Marina K. Abuladze et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Abuladze, Marina K. Sokhadze, Victor M. Namchevadze, Emma N. Kiziria, E. Tabatadze, Leila V. Lejava, Lia V. Gogichaishvili, Sh. Bakradze, Nugzar B. Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress |
title | Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress |
title_full | Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress |
title_fullStr | Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress |
title_full_unstemmed | Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress |
title_short | Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress |
title_sort | thermal analysis of whole bacterial cells exposed to potassium permanganate using differential scanning calorimetry: a biphasic dose-dependent response to stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823177/ https://www.ncbi.nlm.nih.gov/pubmed/19219375 http://dx.doi.org/10.1100/tsw.2009.3 |
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