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Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea

False-negative results for Plasmodium falciparum histidine-rich protein (HRP) 2–based rapid diagnostic tests (RDTs) are increasing in Eritrea. We investigated HRP gene 2/3 (pfhrp2/pfhrp3) status in 50 infected patients at 2 hospitals. We showed that 80.8% (21/26) of patients at Ghindae Hospital and...

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Autores principales: Berhane, Araia, Anderson, Karen, Mihreteab, Selam, Gresty, Karryn, Rogier, Eric, Mohamed, Salih, Hagos, Filmon, Embaye, Ghirmay, Chinorumba, Anderson, Zehaie, Assefash, Dowd, Simone, Waters, Norman C., Gatton, Michelle L., Udhayakumar, Venkatachalam, Cheng, Qin, Cunningham, Jane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823352/
https://www.ncbi.nlm.nih.gov/pubmed/29460730
http://dx.doi.org/10.3201/eid2403.171723
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author Berhane, Araia
Anderson, Karen
Mihreteab, Selam
Gresty, Karryn
Rogier, Eric
Mohamed, Salih
Hagos, Filmon
Embaye, Ghirmay
Chinorumba, Anderson
Zehaie, Assefash
Dowd, Simone
Waters, Norman C.
Gatton, Michelle L.
Udhayakumar, Venkatachalam
Cheng, Qin
Cunningham, Jane
author_facet Berhane, Araia
Anderson, Karen
Mihreteab, Selam
Gresty, Karryn
Rogier, Eric
Mohamed, Salih
Hagos, Filmon
Embaye, Ghirmay
Chinorumba, Anderson
Zehaie, Assefash
Dowd, Simone
Waters, Norman C.
Gatton, Michelle L.
Udhayakumar, Venkatachalam
Cheng, Qin
Cunningham, Jane
author_sort Berhane, Araia
collection PubMed
description False-negative results for Plasmodium falciparum histidine-rich protein (HRP) 2–based rapid diagnostic tests (RDTs) are increasing in Eritrea. We investigated HRP gene 2/3 (pfhrp2/pfhrp3) status in 50 infected patients at 2 hospitals. We showed that 80.8% (21/26) of patients at Ghindae Hospital and 41.7% (10/24) at Massawa Hospital were infected with pfhrp2-negative parasites and 92.3% (24/26) of patients at Ghindae Hospital and 70.8% (17/24) at Massawa Hospital were infected with pfhrp3-negative parasites. Parasite densities between pfhrp2-positive and pfhrp2-negative patients were comparable. All pfhrp2-negative samples had no detectable HRP2/3 antigen and showed negative results for HRP2-based RDTs. pfhrp2-negative parasites were genetically less diverse and formed 2 clusters with no close relationships to parasites from Peru. These parasites probably emerged independently by selection in Eritrea. High prevalence of pfhrp2-negative parasites caused a high rate of false-negative results for RDTs. Determining prevalence of pfhrp2-negative parasites is urgently needed in neighboring countries to assist case management policies.
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spelling pubmed-58233522018-03-01 Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea Berhane, Araia Anderson, Karen Mihreteab, Selam Gresty, Karryn Rogier, Eric Mohamed, Salih Hagos, Filmon Embaye, Ghirmay Chinorumba, Anderson Zehaie, Assefash Dowd, Simone Waters, Norman C. Gatton, Michelle L. Udhayakumar, Venkatachalam Cheng, Qin Cunningham, Jane Emerg Infect Dis Research False-negative results for Plasmodium falciparum histidine-rich protein (HRP) 2–based rapid diagnostic tests (RDTs) are increasing in Eritrea. We investigated HRP gene 2/3 (pfhrp2/pfhrp3) status in 50 infected patients at 2 hospitals. We showed that 80.8% (21/26) of patients at Ghindae Hospital and 41.7% (10/24) at Massawa Hospital were infected with pfhrp2-negative parasites and 92.3% (24/26) of patients at Ghindae Hospital and 70.8% (17/24) at Massawa Hospital were infected with pfhrp3-negative parasites. Parasite densities between pfhrp2-positive and pfhrp2-negative patients were comparable. All pfhrp2-negative samples had no detectable HRP2/3 antigen and showed negative results for HRP2-based RDTs. pfhrp2-negative parasites were genetically less diverse and formed 2 clusters with no close relationships to parasites from Peru. These parasites probably emerged independently by selection in Eritrea. High prevalence of pfhrp2-negative parasites caused a high rate of false-negative results for RDTs. Determining prevalence of pfhrp2-negative parasites is urgently needed in neighboring countries to assist case management policies. Centers for Disease Control and Prevention 2018-03 /pmc/articles/PMC5823352/ /pubmed/29460730 http://dx.doi.org/10.3201/eid2403.171723 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Berhane, Araia
Anderson, Karen
Mihreteab, Selam
Gresty, Karryn
Rogier, Eric
Mohamed, Salih
Hagos, Filmon
Embaye, Ghirmay
Chinorumba, Anderson
Zehaie, Assefash
Dowd, Simone
Waters, Norman C.
Gatton, Michelle L.
Udhayakumar, Venkatachalam
Cheng, Qin
Cunningham, Jane
Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea
title Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea
title_full Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea
title_fullStr Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea
title_full_unstemmed Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea
title_short Major Threat to Malaria Control Programs by Plasmodium falciparum Lacking Histidine-Rich Protein 2, Eritrea
title_sort major threat to malaria control programs by plasmodium falciparum lacking histidine-rich protein 2, eritrea
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823352/
https://www.ncbi.nlm.nih.gov/pubmed/29460730
http://dx.doi.org/10.3201/eid2403.171723
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