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Xenopus: An alternative model system for identifying muco-active agents
The airway epithelium in human plays a central role as the first line of defense against environmental contaminants. Most respiratory diseases such as chronic obstructive pulmonary disease (COPD), asthma, and respiratory infections, disturb normal muco-ciliary functions by stimulating the hypersecre...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823443/ https://www.ncbi.nlm.nih.gov/pubmed/29470529 http://dx.doi.org/10.1371/journal.pone.0193310 |
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author | Sim, Hyo Jung Kim, Sang-Hyun Myung, Kyung-Jae Kwon, Taejoon Lee, Hyun-Shik Park, Tae Joo |
author_facet | Sim, Hyo Jung Kim, Sang-Hyun Myung, Kyung-Jae Kwon, Taejoon Lee, Hyun-Shik Park, Tae Joo |
author_sort | Sim, Hyo Jung |
collection | PubMed |
description | The airway epithelium in human plays a central role as the first line of defense against environmental contaminants. Most respiratory diseases such as chronic obstructive pulmonary disease (COPD), asthma, and respiratory infections, disturb normal muco-ciliary functions by stimulating the hypersecretion of mucus. Several muco-active agents have been used to treat hypersecretion symptoms in patients. Current muco-active reagents control mucus secretion by modulating either airway inflammation, cholinergic parasympathetic nerve activities or by reducing the viscosity by cleaving crosslinking in mucin and digesting DNAs in mucus. However, none of the current medication regulates mucus secretion by directly targeting airway goblet cells. The major hurdle for screening potential muco-active agents that directly affect the goblet cells, is the unavailability of in vivo model systems suitable for high-throughput screening. In this study, we developed a high-throughput in vivo model system for identifying muco-active reagents using Xenopus laevis embryos. We tested mucus secretion under various conditions and developed a screening strategy to identify potential muco-regulators. Using this novel screening technique, we identified narasin as a potential muco-regulator. Narasin treatment of developing Xenopus embryos significantly reduced mucus secretion. Furthermore, the human lung epithelial cell line, Calu-3, responded similarly to narasin treatment, validating our technique for discovering muco-active reagents. |
format | Online Article Text |
id | pubmed-5823443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58234432018-03-15 Xenopus: An alternative model system for identifying muco-active agents Sim, Hyo Jung Kim, Sang-Hyun Myung, Kyung-Jae Kwon, Taejoon Lee, Hyun-Shik Park, Tae Joo PLoS One Research Article The airway epithelium in human plays a central role as the first line of defense against environmental contaminants. Most respiratory diseases such as chronic obstructive pulmonary disease (COPD), asthma, and respiratory infections, disturb normal muco-ciliary functions by stimulating the hypersecretion of mucus. Several muco-active agents have been used to treat hypersecretion symptoms in patients. Current muco-active reagents control mucus secretion by modulating either airway inflammation, cholinergic parasympathetic nerve activities or by reducing the viscosity by cleaving crosslinking in mucin and digesting DNAs in mucus. However, none of the current medication regulates mucus secretion by directly targeting airway goblet cells. The major hurdle for screening potential muco-active agents that directly affect the goblet cells, is the unavailability of in vivo model systems suitable for high-throughput screening. In this study, we developed a high-throughput in vivo model system for identifying muco-active reagents using Xenopus laevis embryos. We tested mucus secretion under various conditions and developed a screening strategy to identify potential muco-regulators. Using this novel screening technique, we identified narasin as a potential muco-regulator. Narasin treatment of developing Xenopus embryos significantly reduced mucus secretion. Furthermore, the human lung epithelial cell line, Calu-3, responded similarly to narasin treatment, validating our technique for discovering muco-active reagents. Public Library of Science 2018-02-22 /pmc/articles/PMC5823443/ /pubmed/29470529 http://dx.doi.org/10.1371/journal.pone.0193310 Text en © 2018 Sim et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sim, Hyo Jung Kim, Sang-Hyun Myung, Kyung-Jae Kwon, Taejoon Lee, Hyun-Shik Park, Tae Joo Xenopus: An alternative model system for identifying muco-active agents |
title | Xenopus: An alternative model system for identifying muco-active agents |
title_full | Xenopus: An alternative model system for identifying muco-active agents |
title_fullStr | Xenopus: An alternative model system for identifying muco-active agents |
title_full_unstemmed | Xenopus: An alternative model system for identifying muco-active agents |
title_short | Xenopus: An alternative model system for identifying muco-active agents |
title_sort | xenopus: an alternative model system for identifying muco-active agents |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823443/ https://www.ncbi.nlm.nih.gov/pubmed/29470529 http://dx.doi.org/10.1371/journal.pone.0193310 |
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