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Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease

AIM: To compare gut bacterial diversity and amount of Enterobacteriaceae in colonic mucosa between patients with and without diverticular disease (DD). METHODS: Patients in a stable clinical condition with planned elective colonoscopy were included. Blood samples and colon mucosa biopsies were colle...

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Autores principales: Linninge, Caroline, Roth, Bodil, Erlanson-Albertsson, Charlotte, Molin, Göran, Toth, Ervin, Ohlsson, Bodil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823699/
https://www.ncbi.nlm.nih.gov/pubmed/29487763
http://dx.doi.org/10.4291/wjgp.v9.i1.18
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author Linninge, Caroline
Roth, Bodil
Erlanson-Albertsson, Charlotte
Molin, Göran
Toth, Ervin
Ohlsson, Bodil
author_facet Linninge, Caroline
Roth, Bodil
Erlanson-Albertsson, Charlotte
Molin, Göran
Toth, Ervin
Ohlsson, Bodil
author_sort Linninge, Caroline
collection PubMed
description AIM: To compare gut bacterial diversity and amount of Enterobacteriaceae in colonic mucosa between patients with and without diverticular disease (DD). METHODS: Patients in a stable clinical condition with planned elective colonoscopy were included. Blood samples and colon mucosa biopsies were collected at the colonoscopy. Study questionnaires including questions about gastrointestinal symptoms were completed by the patients and physicians. DNA from mucosa samples was isolated and the amount of Enterobacteriaceae was estimated using PCR assay. Terminal restriction fragment length polymorphism was applied to assess microbial diversity. Diversity was estimated by calculations of richness (number of terminal restriction fragments) and Shannon-Wiener and Simpson’s indices. RESULTS: A total of 51 patients were included, 16 patients with DD [68 (62-76) years] and 35 controls [62 (40-74) years] without any diverticula. Patients with DD had significantly higher levels of Enterobacteriaceae than those without DD (P = 0.043), and there was an inverse relationship between the amount of Enterobacteriaceae and the Simpson’s index (rs = -0.361, P = 0.033) and the Shannon-Wiener index (rs = -0.299, P = 0.081). The Simpson’s index (P = 0.383), Shannon-Wiener index (P = 0.401) or number of restrictions fragments (P = 0.776) did not differ between DD and controls. The majority of patients experienced gastrointestinal symptoms, and 22 patients (43.1%) fulfilled the criteria for irritable bowel syndrome, with no difference between the groups (P = 0.212). Demography, socioeconomic status, lifestyle habits, inflammatory biomarkers, or symptoms were not related to the amount of Enterobacteriaceae or bacterial diversity. CONCLUSION: Patients with DD had higher amount of Enterobacteriaceae in the colon mucosa compared to patients without diverticula.
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spelling pubmed-58236992018-02-27 Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease Linninge, Caroline Roth, Bodil Erlanson-Albertsson, Charlotte Molin, Göran Toth, Ervin Ohlsson, Bodil World J Gastrointest Pathophysiol Observational Study AIM: To compare gut bacterial diversity and amount of Enterobacteriaceae in colonic mucosa between patients with and without diverticular disease (DD). METHODS: Patients in a stable clinical condition with planned elective colonoscopy were included. Blood samples and colon mucosa biopsies were collected at the colonoscopy. Study questionnaires including questions about gastrointestinal symptoms were completed by the patients and physicians. DNA from mucosa samples was isolated and the amount of Enterobacteriaceae was estimated using PCR assay. Terminal restriction fragment length polymorphism was applied to assess microbial diversity. Diversity was estimated by calculations of richness (number of terminal restriction fragments) and Shannon-Wiener and Simpson’s indices. RESULTS: A total of 51 patients were included, 16 patients with DD [68 (62-76) years] and 35 controls [62 (40-74) years] without any diverticula. Patients with DD had significantly higher levels of Enterobacteriaceae than those without DD (P = 0.043), and there was an inverse relationship between the amount of Enterobacteriaceae and the Simpson’s index (rs = -0.361, P = 0.033) and the Shannon-Wiener index (rs = -0.299, P = 0.081). The Simpson’s index (P = 0.383), Shannon-Wiener index (P = 0.401) or number of restrictions fragments (P = 0.776) did not differ between DD and controls. The majority of patients experienced gastrointestinal symptoms, and 22 patients (43.1%) fulfilled the criteria for irritable bowel syndrome, with no difference between the groups (P = 0.212). Demography, socioeconomic status, lifestyle habits, inflammatory biomarkers, or symptoms were not related to the amount of Enterobacteriaceae or bacterial diversity. CONCLUSION: Patients with DD had higher amount of Enterobacteriaceae in the colon mucosa compared to patients without diverticula. Baishideng Publishing Group Inc 2018-02-15 2018-02-15 /pmc/articles/PMC5823699/ /pubmed/29487763 http://dx.doi.org/10.4291/wjgp.v9.i1.18 Text en ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Observational Study
Linninge, Caroline
Roth, Bodil
Erlanson-Albertsson, Charlotte
Molin, Göran
Toth, Ervin
Ohlsson, Bodil
Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease
title Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease
title_full Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease
title_fullStr Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease
title_full_unstemmed Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease
title_short Abundance of Enterobacteriaceae in the colon mucosa in diverticular disease
title_sort abundance of enterobacteriaceae in the colon mucosa in diverticular disease
topic Observational Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823699/
https://www.ncbi.nlm.nih.gov/pubmed/29487763
http://dx.doi.org/10.4291/wjgp.v9.i1.18
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