Cargando…

Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue

Ice easily recrystallizes during warming after vitrification, and antifreeze protein (AFP) can inhibit the re-crystallization. However, no study has evaluated the effect of AFP treatment only thereon during warming. This study sought to compare AFP treatment protocols: a conventional protocol with A...

Descripción completa

Detalles Bibliográficos
Autores principales: Kong, Hyun Sun, Kim, Eun Jung, Youm, Hye Won, Kim, Seul Ki, Lee, Jung Ryeol, Suh, Chang Suk, Kim, Seok Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Yonsei University College of Medicine 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823838/
https://www.ncbi.nlm.nih.gov/pubmed/29436204
http://dx.doi.org/10.3349/ymj.2018.59.2.331
_version_ 1783301936843325440
author Kong, Hyun Sun
Kim, Eun Jung
Youm, Hye Won
Kim, Seul Ki
Lee, Jung Ryeol
Suh, Chang Suk
Kim, Seok Hyun
author_facet Kong, Hyun Sun
Kim, Eun Jung
Youm, Hye Won
Kim, Seul Ki
Lee, Jung Ryeol
Suh, Chang Suk
Kim, Seok Hyun
author_sort Kong, Hyun Sun
collection PubMed
description Ice easily recrystallizes during warming after vitrification, and antifreeze protein (AFP) can inhibit the re-crystallization. However, no study has evaluated the effect of AFP treatment only thereon during warming. This study sought to compare AFP treatment protocols: a conventional protocol with AFP treatment during vitrification and first-step warming and a new protocol with AFP treatment during the first-step warming only. According to the protocols, 10 mg/mL of LeIBP (a type of AFP) was used. Five-week-old B6D2F1 mouse ovaries were randomly divided into a vitrified-warmed control and two experimental groups, one treated with the conventional AFP treatment protocol (LeIBP-all) and the other with the new AFP treatment protocol (LeIBP-w). For evaluation, ratios of ovarian follicle integrity, apoptosis, and DNA double-strand (DDS) damage/repairing were analyzed. The LeIBP-treated groups showed significantly higher intact follicle ratios than the control, and the results were similar between the LeIBP-treated groups. Apoptotic follicle ratios were significantly lower in both LeIBP-treated groups than the control, and the results were not significantly different between the LeIBP-treated groups. With regard to DDS damage/repairing follicle ratio, significantly lower ratios were recorded in both LeIBP-treated groups, compared to the control, and the results were similar between the LeIBP-treated groups. This study demonstrated that both protocols with LeIBP had a beneficial effect on maintaining follicle integrity and preventing follicle apoptosis and DDS damage. Moreover, the new protocol showed similar results to the conventional protocol. This new protocol could optimize the mouse ovary vitrification-warming procedure using AFP, while minimizing the treatment steps.
format Online
Article
Text
id pubmed-5823838
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Yonsei University College of Medicine
record_format MEDLINE/PubMed
spelling pubmed-58238382018-03-01 Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue Kong, Hyun Sun Kim, Eun Jung Youm, Hye Won Kim, Seul Ki Lee, Jung Ryeol Suh, Chang Suk Kim, Seok Hyun Yonsei Med J Brief Communication Ice easily recrystallizes during warming after vitrification, and antifreeze protein (AFP) can inhibit the re-crystallization. However, no study has evaluated the effect of AFP treatment only thereon during warming. This study sought to compare AFP treatment protocols: a conventional protocol with AFP treatment during vitrification and first-step warming and a new protocol with AFP treatment during the first-step warming only. According to the protocols, 10 mg/mL of LeIBP (a type of AFP) was used. Five-week-old B6D2F1 mouse ovaries were randomly divided into a vitrified-warmed control and two experimental groups, one treated with the conventional AFP treatment protocol (LeIBP-all) and the other with the new AFP treatment protocol (LeIBP-w). For evaluation, ratios of ovarian follicle integrity, apoptosis, and DNA double-strand (DDS) damage/repairing were analyzed. The LeIBP-treated groups showed significantly higher intact follicle ratios than the control, and the results were similar between the LeIBP-treated groups. Apoptotic follicle ratios were significantly lower in both LeIBP-treated groups than the control, and the results were not significantly different between the LeIBP-treated groups. With regard to DDS damage/repairing follicle ratio, significantly lower ratios were recorded in both LeIBP-treated groups, compared to the control, and the results were similar between the LeIBP-treated groups. This study demonstrated that both protocols with LeIBP had a beneficial effect on maintaining follicle integrity and preventing follicle apoptosis and DDS damage. Moreover, the new protocol showed similar results to the conventional protocol. This new protocol could optimize the mouse ovary vitrification-warming procedure using AFP, while minimizing the treatment steps. Yonsei University College of Medicine 2018-03-01 2017-02-05 /pmc/articles/PMC5823838/ /pubmed/29436204 http://dx.doi.org/10.3349/ymj.2018.59.2.331 Text en © Copyright: Yonsei University College of Medicine 2018 http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Kong, Hyun Sun
Kim, Eun Jung
Youm, Hye Won
Kim, Seul Ki
Lee, Jung Ryeol
Suh, Chang Suk
Kim, Seok Hyun
Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue
title Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue
title_full Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue
title_fullStr Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue
title_full_unstemmed Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue
title_short Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue
title_sort improvement in ovarian tissue quality with supplementation of antifreeze protein during warming of vitrified mouse ovarian tissue
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823838/
https://www.ncbi.nlm.nih.gov/pubmed/29436204
http://dx.doi.org/10.3349/ymj.2018.59.2.331
work_keys_str_mv AT konghyunsun improvementinovariantissuequalitywithsupplementationofantifreezeproteinduringwarmingofvitrifiedmouseovariantissue
AT kimeunjung improvementinovariantissuequalitywithsupplementationofantifreezeproteinduringwarmingofvitrifiedmouseovariantissue
AT youmhyewon improvementinovariantissuequalitywithsupplementationofantifreezeproteinduringwarmingofvitrifiedmouseovariantissue
AT kimseulki improvementinovariantissuequalitywithsupplementationofantifreezeproteinduringwarmingofvitrifiedmouseovariantissue
AT leejungryeol improvementinovariantissuequalitywithsupplementationofantifreezeproteinduringwarmingofvitrifiedmouseovariantissue
AT suhchangsuk improvementinovariantissuequalitywithsupplementationofantifreezeproteinduringwarmingofvitrifiedmouseovariantissue
AT kimseokhyun improvementinovariantissuequalitywithsupplementationofantifreezeproteinduringwarmingofvitrifiedmouseovariantissue