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Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters
Environmental surveillance of poliovirus (PV) plays an important role in the global program for eradication of wild PV. The bag-mediated filtration system (BMFS) was first developed in 2014 and enhances PV surveillance when compared to the two-phase grab method currently recommended by the World Hea...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823955/ https://www.ncbi.nlm.nih.gov/pubmed/28674934 http://dx.doi.org/10.1007/s12560-017-9311-7 |
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author | Fagnant, Christine Susan Sánchez-Gonzalez, Liliana Margarita Zhou, Nicolette A. Falman, Jill Christin Eisenstein, Michael Guelig, Dylan Ockerman, Byron Guan, Yifei Kossik, Alexandra Lynn Linden, Yarrow S. Beck, Nicola Koren Wilmouth, Robyn Komen, Evans Mwangi, Benlick Nyangao, James Shirai, Jeffry H. Novosselov, Igor Borus, Peter Boyle, David S. Meschke, John Scott |
author_facet | Fagnant, Christine Susan Sánchez-Gonzalez, Liliana Margarita Zhou, Nicolette A. Falman, Jill Christin Eisenstein, Michael Guelig, Dylan Ockerman, Byron Guan, Yifei Kossik, Alexandra Lynn Linden, Yarrow S. Beck, Nicola Koren Wilmouth, Robyn Komen, Evans Mwangi, Benlick Nyangao, James Shirai, Jeffry H. Novosselov, Igor Borus, Peter Boyle, David S. Meschke, John Scott |
author_sort | Fagnant, Christine Susan |
collection | PubMed |
description | Environmental surveillance of poliovirus (PV) plays an important role in the global program for eradication of wild PV. The bag-mediated filtration system (BMFS) was first developed in 2014 and enhances PV surveillance when compared to the two-phase grab method currently recommended by the World Health Organization (WHO). In this study, the BMFS design was improved and tested for its usability in wastewater and wastewater-impacted surface waters in Nairobi, Kenya. Modifications made to the BMFS included the size, color, and shape of the collection bags, the filter housing used, and the device used to elute the samples from the filters. The modified BMFS concentrated 3–10 L down to 10 mL, which resulted in an effective volume assayed (900–3000 mL) that was 6–20 times greater than the effective volume assayed for samples processed by the WHO algorithm (150 mL). The system developed allows for sampling and in-field virus concentration, followed by transportation of the filter for further analysis with simpler logistics than the current methods. This may ultimately reduce the likelihood of false-negative samples by increasing the effective volume assayed compared to samples processed by the WHO algorithm, making the BMFS a valuable sampling system for wastewater and wastewater-impacted surface waters. |
format | Online Article Text |
id | pubmed-5823955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-58239552018-02-28 Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters Fagnant, Christine Susan Sánchez-Gonzalez, Liliana Margarita Zhou, Nicolette A. Falman, Jill Christin Eisenstein, Michael Guelig, Dylan Ockerman, Byron Guan, Yifei Kossik, Alexandra Lynn Linden, Yarrow S. Beck, Nicola Koren Wilmouth, Robyn Komen, Evans Mwangi, Benlick Nyangao, James Shirai, Jeffry H. Novosselov, Igor Borus, Peter Boyle, David S. Meschke, John Scott Food Environ Virol Original Paper Environmental surveillance of poliovirus (PV) plays an important role in the global program for eradication of wild PV. The bag-mediated filtration system (BMFS) was first developed in 2014 and enhances PV surveillance when compared to the two-phase grab method currently recommended by the World Health Organization (WHO). In this study, the BMFS design was improved and tested for its usability in wastewater and wastewater-impacted surface waters in Nairobi, Kenya. Modifications made to the BMFS included the size, color, and shape of the collection bags, the filter housing used, and the device used to elute the samples from the filters. The modified BMFS concentrated 3–10 L down to 10 mL, which resulted in an effective volume assayed (900–3000 mL) that was 6–20 times greater than the effective volume assayed for samples processed by the WHO algorithm (150 mL). The system developed allows for sampling and in-field virus concentration, followed by transportation of the filter for further analysis with simpler logistics than the current methods. This may ultimately reduce the likelihood of false-negative samples by increasing the effective volume assayed compared to samples processed by the WHO algorithm, making the BMFS a valuable sampling system for wastewater and wastewater-impacted surface waters. Springer US 2017-07-03 2018 /pmc/articles/PMC5823955/ /pubmed/28674934 http://dx.doi.org/10.1007/s12560-017-9311-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Fagnant, Christine Susan Sánchez-Gonzalez, Liliana Margarita Zhou, Nicolette A. Falman, Jill Christin Eisenstein, Michael Guelig, Dylan Ockerman, Byron Guan, Yifei Kossik, Alexandra Lynn Linden, Yarrow S. Beck, Nicola Koren Wilmouth, Robyn Komen, Evans Mwangi, Benlick Nyangao, James Shirai, Jeffry H. Novosselov, Igor Borus, Peter Boyle, David S. Meschke, John Scott Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters |
title | Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters |
title_full | Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters |
title_fullStr | Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters |
title_full_unstemmed | Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters |
title_short | Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters |
title_sort | improvement of the bag-mediated filtration system for sampling wastewater and wastewater-impacted waters |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823955/ https://www.ncbi.nlm.nih.gov/pubmed/28674934 http://dx.doi.org/10.1007/s12560-017-9311-7 |
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