Cargando…

Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin

microRNA (miR)-612 shows anticancer activity in several types of cancers, yet its function in melanoma is still unclear. This study was undertaken to investigate the expression of miR-612 and its biological relevance in melanoma cell growth, invasion, and tumorigenesis. The expression and prognostic...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Ying, Zhang, Hao-liang, Wang, Qi-ying, Chen, Min-jing, Liu, Lin-bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824021/
https://www.ncbi.nlm.nih.gov/pubmed/29385671
http://dx.doi.org/10.14348/molcells.2018.2235
_version_ 1783301979019149312
author Zhu, Ying
Zhang, Hao-liang
Wang, Qi-ying
Chen, Min-jing
Liu, Lin-bo
author_facet Zhu, Ying
Zhang, Hao-liang
Wang, Qi-ying
Chen, Min-jing
Liu, Lin-bo
author_sort Zhu, Ying
collection PubMed
description microRNA (miR)-612 shows anticancer activity in several types of cancers, yet its function in melanoma is still unclear. This study was undertaken to investigate the expression of miR-612 and its biological relevance in melanoma cell growth, invasion, and tumorigenesis. The expression and prognostic significance of miR-612 in melanoma were examined. The effects of miR-612 overexpression on cell proliferation, colony formation, tumorigenesis, and invasion were determined. Rescue experiments were conducted to identify the functional target gene(s) of miR-612. miR-612 was significantly downregulated in melanoma tissues compared to adjacent normal tissues. Low miR-612 expression was significantly associated with melanoma thickness, lymph node metastasis, and shorter overall, and disease-free survival of patients. Overexpression of miR-612 significantly decreased cell proliferation, colony formation, and invasion of SK-MEL-28 and A375 melanoma cells. In vivo tumorigenic studies confirmed that miR-612 overexpression retarded the growth of A375 xenograft tumors, which was coupled with a decline in the percentage of Ki-67-positive proliferating cells. Mechanistically, miR-612 targeted Espin in melanoma cells. Overexpression of Espin counteracted the suppressive effects of miR-612 on melanoma cell proliferation, invasion, and tumorigenesis. A significant inverse correlation (r = −0.376, P = 0.018) was observed between miR-612 and Espin protein expression in melanoma tissues. In addition, overexpression of miR-612 and knockdown of Espin significantly increased the sensitivity of melanoma cells to doxorubicin. Collectively, miR-612 suppresses the aggressive phenotype of melanoma cells through downregulation of Espin. Delivery of miR-612 may represent a novel therapeutic strategy against melanoma.
format Online
Article
Text
id pubmed-5824021
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Korean Society for Molecular and Cellular Biology
record_format MEDLINE/PubMed
spelling pubmed-58240212018-02-28 Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin Zhu, Ying Zhang, Hao-liang Wang, Qi-ying Chen, Min-jing Liu, Lin-bo Mol Cells Article microRNA (miR)-612 shows anticancer activity in several types of cancers, yet its function in melanoma is still unclear. This study was undertaken to investigate the expression of miR-612 and its biological relevance in melanoma cell growth, invasion, and tumorigenesis. The expression and prognostic significance of miR-612 in melanoma were examined. The effects of miR-612 overexpression on cell proliferation, colony formation, tumorigenesis, and invasion were determined. Rescue experiments were conducted to identify the functional target gene(s) of miR-612. miR-612 was significantly downregulated in melanoma tissues compared to adjacent normal tissues. Low miR-612 expression was significantly associated with melanoma thickness, lymph node metastasis, and shorter overall, and disease-free survival of patients. Overexpression of miR-612 significantly decreased cell proliferation, colony formation, and invasion of SK-MEL-28 and A375 melanoma cells. In vivo tumorigenic studies confirmed that miR-612 overexpression retarded the growth of A375 xenograft tumors, which was coupled with a decline in the percentage of Ki-67-positive proliferating cells. Mechanistically, miR-612 targeted Espin in melanoma cells. Overexpression of Espin counteracted the suppressive effects of miR-612 on melanoma cell proliferation, invasion, and tumorigenesis. A significant inverse correlation (r = −0.376, P = 0.018) was observed between miR-612 and Espin protein expression in melanoma tissues. In addition, overexpression of miR-612 and knockdown of Espin significantly increased the sensitivity of melanoma cells to doxorubicin. Collectively, miR-612 suppresses the aggressive phenotype of melanoma cells through downregulation of Espin. Delivery of miR-612 may represent a novel therapeutic strategy against melanoma. Korean Society for Molecular and Cellular Biology 2018-02-28 2018-01-29 /pmc/articles/PMC5824021/ /pubmed/29385671 http://dx.doi.org/10.14348/molcells.2018.2235 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Article
Zhu, Ying
Zhang, Hao-liang
Wang, Qi-ying
Chen, Min-jing
Liu, Lin-bo
Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin
title Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin
title_full Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin
title_fullStr Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin
title_full_unstemmed Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin
title_short Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin
title_sort overexpression of microrna-612 restrains the growth, invasion, and tumorigenesis of melanoma cells by targeting espin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824021/
https://www.ncbi.nlm.nih.gov/pubmed/29385671
http://dx.doi.org/10.14348/molcells.2018.2235
work_keys_str_mv AT zhuying overexpressionofmicrorna612restrainsthegrowthinvasionandtumorigenesisofmelanomacellsbytargetingespin
AT zhanghaoliang overexpressionofmicrorna612restrainsthegrowthinvasionandtumorigenesisofmelanomacellsbytargetingespin
AT wangqiying overexpressionofmicrorna612restrainsthegrowthinvasionandtumorigenesisofmelanomacellsbytargetingespin
AT chenminjing overexpressionofmicrorna612restrainsthegrowthinvasionandtumorigenesisofmelanomacellsbytargetingespin
AT liulinbo overexpressionofmicrorna612restrainsthegrowthinvasionandtumorigenesisofmelanomacellsbytargetingespin