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PPARα-Target Gene Expression Requires TIS21(/BTG2) Gene in Liver of the C57BL/6 Mice under Fasting Condition
The TIS21(/BTG2/PC3) gene belongs to the antiproliferative gene (APRO) family and exhibits tumor suppressive activity. However, here we report that TIS21 controls lipid metabolism, rather than cell proliferation, under fasting condition. Using microarray analysis, whole gene expression changes were...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Molecular and Cellular Biology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824024/ https://www.ncbi.nlm.nih.gov/pubmed/29385670 http://dx.doi.org/10.14348/molcells.2018.2257 |
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author | Hong, Allen Eugene Ryu, Min Sook Kim, Seung Jun Hwang, Seung Yong Lim, In Kyoung |
author_facet | Hong, Allen Eugene Ryu, Min Sook Kim, Seung Jun Hwang, Seung Yong Lim, In Kyoung |
author_sort | Hong, Allen Eugene |
collection | PubMed |
description | The TIS21(/BTG2/PC3) gene belongs to the antiproliferative gene (APRO) family and exhibits tumor suppressive activity. However, here we report that TIS21 controls lipid metabolism, rather than cell proliferation, under fasting condition. Using microarray analysis, whole gene expression changes were investigated in liver of TIS21 knockout (TIS21-KO) mice after 20 h fasting and compared with wild type (WT). Peroxisome proliferator-activated receptor alpha (PPARα) target gene expression was almost absent in contrast to increased lipid synthesis in the TIS21-KO mice compared to WT mice. Immunohistochemistry with hematoxylin and eosin staining revealed that lipid deposition was focal in the TIS21-KO liver as opposed to the diffuse and homogeneous pattern in the WT liver after 24 h starvation. In addition, cathepsin E expression was over 10 times higher in the TIS21-KO liver than that in the WT, as opposed to the significant reduction of thioltransferase in both adult and fetal livers. At present, we cannot account for the role of cathepsin E. However, downregulation of glutaredoxin 2 thioltransferase expression might affect hypoxic damage in the TIS21-KO liver. We suggest that the TIS21(/BTG2) gene might be essential to maintain energy metabolism and reducing power in the liver under fasting condition. |
format | Online Article Text |
id | pubmed-5824024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Korean Society for Molecular and Cellular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-58240242018-02-28 PPARα-Target Gene Expression Requires TIS21(/BTG2) Gene in Liver of the C57BL/6 Mice under Fasting Condition Hong, Allen Eugene Ryu, Min Sook Kim, Seung Jun Hwang, Seung Yong Lim, In Kyoung Mol Cells Article The TIS21(/BTG2/PC3) gene belongs to the antiproliferative gene (APRO) family and exhibits tumor suppressive activity. However, here we report that TIS21 controls lipid metabolism, rather than cell proliferation, under fasting condition. Using microarray analysis, whole gene expression changes were investigated in liver of TIS21 knockout (TIS21-KO) mice after 20 h fasting and compared with wild type (WT). Peroxisome proliferator-activated receptor alpha (PPARα) target gene expression was almost absent in contrast to increased lipid synthesis in the TIS21-KO mice compared to WT mice. Immunohistochemistry with hematoxylin and eosin staining revealed that lipid deposition was focal in the TIS21-KO liver as opposed to the diffuse and homogeneous pattern in the WT liver after 24 h starvation. In addition, cathepsin E expression was over 10 times higher in the TIS21-KO liver than that in the WT, as opposed to the significant reduction of thioltransferase in both adult and fetal livers. At present, we cannot account for the role of cathepsin E. However, downregulation of glutaredoxin 2 thioltransferase expression might affect hypoxic damage in the TIS21-KO liver. We suggest that the TIS21(/BTG2) gene might be essential to maintain energy metabolism and reducing power in the liver under fasting condition. Korean Society for Molecular and Cellular Biology 2018-02-28 2018-01-29 /pmc/articles/PMC5824024/ /pubmed/29385670 http://dx.doi.org/10.14348/molcells.2018.2257 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/. |
spellingShingle | Article Hong, Allen Eugene Ryu, Min Sook Kim, Seung Jun Hwang, Seung Yong Lim, In Kyoung PPARα-Target Gene Expression Requires TIS21(/BTG2) Gene in Liver of the C57BL/6 Mice under Fasting Condition |
title | PPARα-Target Gene Expression Requires TIS21(/BTG2) Gene in Liver of the C57BL/6 Mice under Fasting Condition |
title_full | PPARα-Target Gene Expression Requires TIS21(/BTG2) Gene in Liver of the C57BL/6 Mice under Fasting Condition |
title_fullStr | PPARα-Target Gene Expression Requires TIS21(/BTG2) Gene in Liver of the C57BL/6 Mice under Fasting Condition |
title_full_unstemmed | PPARα-Target Gene Expression Requires TIS21(/BTG2) Gene in Liver of the C57BL/6 Mice under Fasting Condition |
title_short | PPARα-Target Gene Expression Requires TIS21(/BTG2) Gene in Liver of the C57BL/6 Mice under Fasting Condition |
title_sort | pparα-target gene expression requires tis21(/btg2) gene in liver of the c57bl/6 mice under fasting condition |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824024/ https://www.ncbi.nlm.nih.gov/pubmed/29385670 http://dx.doi.org/10.14348/molcells.2018.2257 |
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