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MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development

In mammals, commitment and specification of germ cell lines involves complex programs that include sex differentiation, control of proliferation, and meiotic initiation. Regulation of these processes is genetically controlled by fine-tuned mechanisms of gene regulation in which microRNAs (miRNAs) ar...

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Autores principales: Fernández-Pérez, Daniel, Brieño-Enríquez, Miguel A., Isoler-Alcaraz, Javier, Larriba, Eduardo, del Mazo, Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824349/
https://www.ncbi.nlm.nih.gov/pubmed/29187591
http://dx.doi.org/10.1261/rna.062869.117
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author Fernández-Pérez, Daniel
Brieño-Enríquez, Miguel A.
Isoler-Alcaraz, Javier
Larriba, Eduardo
del Mazo, Jesús
author_facet Fernández-Pérez, Daniel
Brieño-Enríquez, Miguel A.
Isoler-Alcaraz, Javier
Larriba, Eduardo
del Mazo, Jesús
author_sort Fernández-Pérez, Daniel
collection PubMed
description In mammals, commitment and specification of germ cell lines involves complex programs that include sex differentiation, control of proliferation, and meiotic initiation. Regulation of these processes is genetically controlled by fine-tuned mechanisms of gene regulation in which microRNAs (miRNAs) are involved. We have characterized, by small-RNA-seq and bioinformatics analyses, the miRNA expression patterns of male and female mouse primordial germ cells (PGCs) and gonadal somatic cells at embryonic stages E11.5, E12.5, and E13.5. Differential expression analyses revealed differences in the regulation of key miRNA clusters such as miR-199-214, miR-182-183-96, and miR-34c-5p, whose targets have defined roles during gonadal sexual determination in both germ and somatic cells. Extensive analyses of miRNA sequences revealed an increase in noncanonical isoforms on PGCs at E12.5 and dramatic changes of 3′ isomiR expression and 3′ nontemplate nucleotide additions in female PGCs at E13.5. Additionally, RT-qPCR analyses of genes encoding proteins involved in miRNA biogenesis and 3′ nucleotide addition uncovered sexually and developmentally specific expression, characterized by the decay of Drosha, Dgcr8, and Xpo5 expression along gonadal development. These results demonstrate that miRNAs, their isomiRs, and miRNA machinery are differentially regulated and participate actively in gonadal sexual differentiation in both PGCs and gonadal somatic cells.
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spelling pubmed-58243492019-03-01 MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development Fernández-Pérez, Daniel Brieño-Enríquez, Miguel A. Isoler-Alcaraz, Javier Larriba, Eduardo del Mazo, Jesús RNA Bioinformatics In mammals, commitment and specification of germ cell lines involves complex programs that include sex differentiation, control of proliferation, and meiotic initiation. Regulation of these processes is genetically controlled by fine-tuned mechanisms of gene regulation in which microRNAs (miRNAs) are involved. We have characterized, by small-RNA-seq and bioinformatics analyses, the miRNA expression patterns of male and female mouse primordial germ cells (PGCs) and gonadal somatic cells at embryonic stages E11.5, E12.5, and E13.5. Differential expression analyses revealed differences in the regulation of key miRNA clusters such as miR-199-214, miR-182-183-96, and miR-34c-5p, whose targets have defined roles during gonadal sexual determination in both germ and somatic cells. Extensive analyses of miRNA sequences revealed an increase in noncanonical isoforms on PGCs at E12.5 and dramatic changes of 3′ isomiR expression and 3′ nontemplate nucleotide additions in female PGCs at E13.5. Additionally, RT-qPCR analyses of genes encoding proteins involved in miRNA biogenesis and 3′ nucleotide addition uncovered sexually and developmentally specific expression, characterized by the decay of Drosha, Dgcr8, and Xpo5 expression along gonadal development. These results demonstrate that miRNAs, their isomiRs, and miRNA machinery are differentially regulated and participate actively in gonadal sexual differentiation in both PGCs and gonadal somatic cells. Cold Spring Harbor Laboratory Press 2018-03 /pmc/articles/PMC5824349/ /pubmed/29187591 http://dx.doi.org/10.1261/rna.062869.117 Text en © 2018 Fernández-Pérez et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Bioinformatics
Fernández-Pérez, Daniel
Brieño-Enríquez, Miguel A.
Isoler-Alcaraz, Javier
Larriba, Eduardo
del Mazo, Jesús
MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development
title MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development
title_full MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development
title_fullStr MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development
title_full_unstemmed MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development
title_short MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development
title_sort microrna dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824349/
https://www.ncbi.nlm.nih.gov/pubmed/29187591
http://dx.doi.org/10.1261/rna.062869.117
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