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Outer dense fibers stabilize the axoneme to maintain sperm motility

Outer dense fibers (ODFs), as unique accessory structures in mammalian sperm, are considered to play a role in the protection of the sperm tail against shear forces. However, the role and relevant mechanisms of ODFs in modulating sperm motility and its pathological involvement in asthenozoospermia w...

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Autores principales: Zhao, Wenlong, Li, Zhengzheng, Ping, Ping, Wang, Guishuan, Yuan, Xiaobing, Sun, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824370/
https://www.ncbi.nlm.nih.gov/pubmed/29168316
http://dx.doi.org/10.1111/jcmm.13457
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author Zhao, Wenlong
Li, Zhengzheng
Ping, Ping
Wang, Guishuan
Yuan, Xiaobing
Sun, Fei
author_facet Zhao, Wenlong
Li, Zhengzheng
Ping, Ping
Wang, Guishuan
Yuan, Xiaobing
Sun, Fei
author_sort Zhao, Wenlong
collection PubMed
description Outer dense fibers (ODFs), as unique accessory structures in mammalian sperm, are considered to play a role in the protection of the sperm tail against shear forces. However, the role and relevant mechanisms of ODFs in modulating sperm motility and its pathological involvement in asthenozoospermia were unknown. Here, we found that the percentage of ODF defects was higher in asthenozoospermic samples than that in control samples and was significantly correlated with the percentage of axoneme defects and non‐motile sperm. Furthermore, the expression levels of ODF major components (Odf1, 2, 3, 4) were frequently down‐regulated in asthenozoospermic samples. Intriguingly, the positive relationship between ODF size and sperm motility existed across species. The conditional disruption of Odf2 expression in mice led to reduced sperm motility and the characteristics of asthenozoospermia. Meanwhile, the expression of acetylated α‐tubulin was decreased in sperm from both Odf2 conditional knockout (cKO) mice and asthenozoospermic men. Immunofluorescence and biochemistry analyses showed that Odf2 could bind to acetylated α‐tubulin and protect the acetylation level of α‐tubulin in HEK293T cells in a cold environment. Finally, we found that lithium elevated the expression levels of Odf family proteins and acetylated α‐tubulin, elongated the midpiece length and increased the percentage of rapidly moving sperm in mice. Our results demonstrate that ODFs are beneficial for sperm motility via stabilization of the axoneme and that hypo‐expression of Odf family proteins is involved in the pathogenesis of asthenozoospermia. The lithium administration assay will provide valuable insights into the development of new treatments for asthenozoospermia.
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spelling pubmed-58243702018-03-01 Outer dense fibers stabilize the axoneme to maintain sperm motility Zhao, Wenlong Li, Zhengzheng Ping, Ping Wang, Guishuan Yuan, Xiaobing Sun, Fei J Cell Mol Med Original Articles Outer dense fibers (ODFs), as unique accessory structures in mammalian sperm, are considered to play a role in the protection of the sperm tail against shear forces. However, the role and relevant mechanisms of ODFs in modulating sperm motility and its pathological involvement in asthenozoospermia were unknown. Here, we found that the percentage of ODF defects was higher in asthenozoospermic samples than that in control samples and was significantly correlated with the percentage of axoneme defects and non‐motile sperm. Furthermore, the expression levels of ODF major components (Odf1, 2, 3, 4) were frequently down‐regulated in asthenozoospermic samples. Intriguingly, the positive relationship between ODF size and sperm motility existed across species. The conditional disruption of Odf2 expression in mice led to reduced sperm motility and the characteristics of asthenozoospermia. Meanwhile, the expression of acetylated α‐tubulin was decreased in sperm from both Odf2 conditional knockout (cKO) mice and asthenozoospermic men. Immunofluorescence and biochemistry analyses showed that Odf2 could bind to acetylated α‐tubulin and protect the acetylation level of α‐tubulin in HEK293T cells in a cold environment. Finally, we found that lithium elevated the expression levels of Odf family proteins and acetylated α‐tubulin, elongated the midpiece length and increased the percentage of rapidly moving sperm in mice. Our results demonstrate that ODFs are beneficial for sperm motility via stabilization of the axoneme and that hypo‐expression of Odf family proteins is involved in the pathogenesis of asthenozoospermia. The lithium administration assay will provide valuable insights into the development of new treatments for asthenozoospermia. John Wiley and Sons Inc. 2017-11-23 2018-03 /pmc/articles/PMC5824370/ /pubmed/29168316 http://dx.doi.org/10.1111/jcmm.13457 Text en © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zhao, Wenlong
Li, Zhengzheng
Ping, Ping
Wang, Guishuan
Yuan, Xiaobing
Sun, Fei
Outer dense fibers stabilize the axoneme to maintain sperm motility
title Outer dense fibers stabilize the axoneme to maintain sperm motility
title_full Outer dense fibers stabilize the axoneme to maintain sperm motility
title_fullStr Outer dense fibers stabilize the axoneme to maintain sperm motility
title_full_unstemmed Outer dense fibers stabilize the axoneme to maintain sperm motility
title_short Outer dense fibers stabilize the axoneme to maintain sperm motility
title_sort outer dense fibers stabilize the axoneme to maintain sperm motility
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824370/
https://www.ncbi.nlm.nih.gov/pubmed/29168316
http://dx.doi.org/10.1111/jcmm.13457
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