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Membrane cholesterol mediates the cellular effects of monolayer graphene substrates
Graphene possesses extraordinary properties that promise great potential in biomedicine. However, fully leveraging these properties requires close contact with the cell surface, raising the concern of unexpected biological consequences. Computational models have demonstrated that graphene preferenti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824811/ https://www.ncbi.nlm.nih.gov/pubmed/29476054 http://dx.doi.org/10.1038/s41467-018-03185-0 |
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author | Kitko, Kristina E. Hong, Tu Lazarenko, Roman M. Ying, Da Xu, Ya-Qiong Zhang, Qi |
author_facet | Kitko, Kristina E. Hong, Tu Lazarenko, Roman M. Ying, Da Xu, Ya-Qiong Zhang, Qi |
author_sort | Kitko, Kristina E. |
collection | PubMed |
description | Graphene possesses extraordinary properties that promise great potential in biomedicine. However, fully leveraging these properties requires close contact with the cell surface, raising the concern of unexpected biological consequences. Computational models have demonstrated that graphene preferentially interacts with cholesterol, a multifunctional lipid unique to eukaryotic membranes. Here we demonstrate an interaction between graphene and cholesterol. We find that graphene increases cell membrane cholesterol and potentiates neurotransmission, which is mediated by increases in the number, release probability, and recycling rate of synaptic vesicles. In fibroblasts grown on graphene, we also find an increase in cholesterol, which promotes the activation of P2Y receptors, a family of receptor regulated by cholesterol. In both cases, direct manipulation of cholesterol levels elucidates that a graphene-induced cholesterol increase underlies the observed potentiation of each cell signaling pathway. These findings identify cholesterol as a mediator of graphene’s cellular effects, providing insight into the biological impact of graphene. |
format | Online Article Text |
id | pubmed-5824811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58248112018-02-26 Membrane cholesterol mediates the cellular effects of monolayer graphene substrates Kitko, Kristina E. Hong, Tu Lazarenko, Roman M. Ying, Da Xu, Ya-Qiong Zhang, Qi Nat Commun Article Graphene possesses extraordinary properties that promise great potential in biomedicine. However, fully leveraging these properties requires close contact with the cell surface, raising the concern of unexpected biological consequences. Computational models have demonstrated that graphene preferentially interacts with cholesterol, a multifunctional lipid unique to eukaryotic membranes. Here we demonstrate an interaction between graphene and cholesterol. We find that graphene increases cell membrane cholesterol and potentiates neurotransmission, which is mediated by increases in the number, release probability, and recycling rate of synaptic vesicles. In fibroblasts grown on graphene, we also find an increase in cholesterol, which promotes the activation of P2Y receptors, a family of receptor regulated by cholesterol. In both cases, direct manipulation of cholesterol levels elucidates that a graphene-induced cholesterol increase underlies the observed potentiation of each cell signaling pathway. These findings identify cholesterol as a mediator of graphene’s cellular effects, providing insight into the biological impact of graphene. Nature Publishing Group UK 2018-02-23 /pmc/articles/PMC5824811/ /pubmed/29476054 http://dx.doi.org/10.1038/s41467-018-03185-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kitko, Kristina E. Hong, Tu Lazarenko, Roman M. Ying, Da Xu, Ya-Qiong Zhang, Qi Membrane cholesterol mediates the cellular effects of monolayer graphene substrates |
title | Membrane cholesterol mediates the cellular effects of monolayer graphene substrates |
title_full | Membrane cholesterol mediates the cellular effects of monolayer graphene substrates |
title_fullStr | Membrane cholesterol mediates the cellular effects of monolayer graphene substrates |
title_full_unstemmed | Membrane cholesterol mediates the cellular effects of monolayer graphene substrates |
title_short | Membrane cholesterol mediates the cellular effects of monolayer graphene substrates |
title_sort | membrane cholesterol mediates the cellular effects of monolayer graphene substrates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824811/ https://www.ncbi.nlm.nih.gov/pubmed/29476054 http://dx.doi.org/10.1038/s41467-018-03185-0 |
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