Foxm1 controls a pro-stemness microRNA network in neural stem cells

Cerebellar neural stem cells (NSCs) require Hedgehog-Gli (Hh-Gli) signalling for their maintenance and Nanog expression for their self-renewal. To identify novel molecular features of this regulatory pathway, we used next-generation sequencing technology to profile mRNA and microRNA expression in ce...

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Autores principales: Besharat, Zein Mersini, Abballe, Luana, Cicconardi, Francesco, Bhutkar, Arjun, Grassi, Luigi, Le Pera, Loredana, Moretti, Marta, Chinappi, Mauro, D’Andrea, Daniel, Mastronuzzi, Angela, Ianari, Alessandra, Vacca, Alessandra, De Smaele, Enrico, Locatelli, Franco, Po, Agnese, Miele, Evelina, Ferretti, Elisabetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824884/
https://www.ncbi.nlm.nih.gov/pubmed/29476172
http://dx.doi.org/10.1038/s41598-018-21876-y
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author Besharat, Zein Mersini
Abballe, Luana
Cicconardi, Francesco
Bhutkar, Arjun
Grassi, Luigi
Le Pera, Loredana
Moretti, Marta
Chinappi, Mauro
D’Andrea, Daniel
Mastronuzzi, Angela
Ianari, Alessandra
Vacca, Alessandra
De Smaele, Enrico
Locatelli, Franco
Po, Agnese
Miele, Evelina
Ferretti, Elisabetta
author_facet Besharat, Zein Mersini
Abballe, Luana
Cicconardi, Francesco
Bhutkar, Arjun
Grassi, Luigi
Le Pera, Loredana
Moretti, Marta
Chinappi, Mauro
D’Andrea, Daniel
Mastronuzzi, Angela
Ianari, Alessandra
Vacca, Alessandra
De Smaele, Enrico
Locatelli, Franco
Po, Agnese
Miele, Evelina
Ferretti, Elisabetta
author_sort Besharat, Zein Mersini
collection PubMed
description Cerebellar neural stem cells (NSCs) require Hedgehog-Gli (Hh-Gli) signalling for their maintenance and Nanog expression for their self-renewal. To identify novel molecular features of this regulatory pathway, we used next-generation sequencing technology to profile mRNA and microRNA expression in cerebellar NSCs, before and after induced differentiation (Diff-NSCs). Genes with higher transcript levels in NSCs (vs. Diff-NSCs) included Foxm1, which proved to be directly regulated by Gli and Nanog. Foxm1 in turn regulated several microRNAs that were overexpressed in NSCs: miR-130b, miR-301a, and members of the miR-15~16 and miR-17~92 clusters and whose knockdown significantly impaired the neurosphere formation ability. Our results reveal a novel Hh-Gli-Nanog-driven Foxm1-microRNA network that controls the self-renewal capacity of NSCs.
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spelling pubmed-58248842018-03-01 Foxm1 controls a pro-stemness microRNA network in neural stem cells Besharat, Zein Mersini Abballe, Luana Cicconardi, Francesco Bhutkar, Arjun Grassi, Luigi Le Pera, Loredana Moretti, Marta Chinappi, Mauro D’Andrea, Daniel Mastronuzzi, Angela Ianari, Alessandra Vacca, Alessandra De Smaele, Enrico Locatelli, Franco Po, Agnese Miele, Evelina Ferretti, Elisabetta Sci Rep Article Cerebellar neural stem cells (NSCs) require Hedgehog-Gli (Hh-Gli) signalling for their maintenance and Nanog expression for their self-renewal. To identify novel molecular features of this regulatory pathway, we used next-generation sequencing technology to profile mRNA and microRNA expression in cerebellar NSCs, before and after induced differentiation (Diff-NSCs). Genes with higher transcript levels in NSCs (vs. Diff-NSCs) included Foxm1, which proved to be directly regulated by Gli and Nanog. Foxm1 in turn regulated several microRNAs that were overexpressed in NSCs: miR-130b, miR-301a, and members of the miR-15~16 and miR-17~92 clusters and whose knockdown significantly impaired the neurosphere formation ability. Our results reveal a novel Hh-Gli-Nanog-driven Foxm1-microRNA network that controls the self-renewal capacity of NSCs. Nature Publishing Group UK 2018-02-23 /pmc/articles/PMC5824884/ /pubmed/29476172 http://dx.doi.org/10.1038/s41598-018-21876-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Besharat, Zein Mersini
Abballe, Luana
Cicconardi, Francesco
Bhutkar, Arjun
Grassi, Luigi
Le Pera, Loredana
Moretti, Marta
Chinappi, Mauro
D’Andrea, Daniel
Mastronuzzi, Angela
Ianari, Alessandra
Vacca, Alessandra
De Smaele, Enrico
Locatelli, Franco
Po, Agnese
Miele, Evelina
Ferretti, Elisabetta
Foxm1 controls a pro-stemness microRNA network in neural stem cells
title Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_full Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_fullStr Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_full_unstemmed Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_short Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_sort foxm1 controls a pro-stemness microrna network in neural stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824884/
https://www.ncbi.nlm.nih.gov/pubmed/29476172
http://dx.doi.org/10.1038/s41598-018-21876-y
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