An integrated microfluidic device for the sorting of yeast cells using image processing

The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usually tedi...

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Autores principales: Yu, Bo Yang, Elbuken, Caglar, Shen, Chong, Huissoon, Jan Paul, Ren, Carolyn L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824950/
https://www.ncbi.nlm.nih.gov/pubmed/29476103
http://dx.doi.org/10.1038/s41598-018-21833-9
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author Yu, Bo Yang
Elbuken, Caglar
Shen, Chong
Huissoon, Jan Paul
Ren, Carolyn L.
author_facet Yu, Bo Yang
Elbuken, Caglar
Shen, Chong
Huissoon, Jan Paul
Ren, Carolyn L.
author_sort Yu, Bo Yang
collection PubMed
description The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usually tedious and inconsistent. This paper presents a microfluidic device integrated with microvalves for fluid control for the sorting of yeast cells using image processing algorithms and confirmation based on their fluorescent tag. The proposed device is completely automated, low cost and easy to implement in an academic research setting. Design details of the integrated microfluidic system are highlighted in this paper, along with experimental validation. Real time cell sorting was demonstrated with a cell detection rate of 12 cells per minute.
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spelling pubmed-58249502018-03-01 An integrated microfluidic device for the sorting of yeast cells using image processing Yu, Bo Yang Elbuken, Caglar Shen, Chong Huissoon, Jan Paul Ren, Carolyn L. Sci Rep Article The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usually tedious and inconsistent. This paper presents a microfluidic device integrated with microvalves for fluid control for the sorting of yeast cells using image processing algorithms and confirmation based on their fluorescent tag. The proposed device is completely automated, low cost and easy to implement in an academic research setting. Design details of the integrated microfluidic system are highlighted in this paper, along with experimental validation. Real time cell sorting was demonstrated with a cell detection rate of 12 cells per minute. Nature Publishing Group UK 2018-02-23 /pmc/articles/PMC5824950/ /pubmed/29476103 http://dx.doi.org/10.1038/s41598-018-21833-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yu, Bo Yang
Elbuken, Caglar
Shen, Chong
Huissoon, Jan Paul
Ren, Carolyn L.
An integrated microfluidic device for the sorting of yeast cells using image processing
title An integrated microfluidic device for the sorting of yeast cells using image processing
title_full An integrated microfluidic device for the sorting of yeast cells using image processing
title_fullStr An integrated microfluidic device for the sorting of yeast cells using image processing
title_full_unstemmed An integrated microfluidic device for the sorting of yeast cells using image processing
title_short An integrated microfluidic device for the sorting of yeast cells using image processing
title_sort integrated microfluidic device for the sorting of yeast cells using image processing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5824950/
https://www.ncbi.nlm.nih.gov/pubmed/29476103
http://dx.doi.org/10.1038/s41598-018-21833-9
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