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Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses
Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during infection have been described, but their utility as a diagnostic marker is still unclear. In this study, we undertook a lipidom...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5825116/ https://www.ncbi.nlm.nih.gov/pubmed/29474474 http://dx.doi.org/10.1371/journal.pone.0193424 |
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author | Wood, Paul L. Steinman, Margaret Erol, Erdal Carter, Craig Christmann, Undine Verma, Ashutosh |
author_facet | Wood, Paul L. Steinman, Margaret Erol, Erdal Carter, Craig Christmann, Undine Verma, Ashutosh |
author_sort | Wood, Paul L. |
collection | PubMed |
description | Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during infection have been described, but their utility as a diagnostic marker is still unclear. In this study, we undertook a lipidomics approach to determine if there are any differences in the serum lipid profiles of horses naturally infected with pathogenic Leptospira spp. and horses vaccinated against a commercially available bacterin. Utilizing a high-resolution mass spectrometry serum lipidomics analytical platform, we demonstrate that cyclic phosphatidic acids, diacylglycerols, and hydroperoxide oxidation products of choline plasmalogens are elevated in the serum of naturally infected as well as vaccinated horses. Other lipids of interest were triacylglycerols that were only elevated in the serum of infected horses and sphingomyelins that were increased only in the serum of vaccinated horses. This is the first report looking at the equine serum lipidome during leptospiral infection and vaccination. |
format | Online Article Text |
id | pubmed-5825116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58251162018-03-19 Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses Wood, Paul L. Steinman, Margaret Erol, Erdal Carter, Craig Christmann, Undine Verma, Ashutosh PLoS One Research Article Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during infection have been described, but their utility as a diagnostic marker is still unclear. In this study, we undertook a lipidomics approach to determine if there are any differences in the serum lipid profiles of horses naturally infected with pathogenic Leptospira spp. and horses vaccinated against a commercially available bacterin. Utilizing a high-resolution mass spectrometry serum lipidomics analytical platform, we demonstrate that cyclic phosphatidic acids, diacylglycerols, and hydroperoxide oxidation products of choline plasmalogens are elevated in the serum of naturally infected as well as vaccinated horses. Other lipids of interest were triacylglycerols that were only elevated in the serum of infected horses and sphingomyelins that were increased only in the serum of vaccinated horses. This is the first report looking at the equine serum lipidome during leptospiral infection and vaccination. Public Library of Science 2018-02-23 /pmc/articles/PMC5825116/ /pubmed/29474474 http://dx.doi.org/10.1371/journal.pone.0193424 Text en © 2018 Wood et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wood, Paul L. Steinman, Margaret Erol, Erdal Carter, Craig Christmann, Undine Verma, Ashutosh Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses |
title | Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses |
title_full | Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses |
title_fullStr | Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses |
title_full_unstemmed | Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses |
title_short | Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses |
title_sort | lipidomic analysis of immune activation in equine leptospirosis and leptospira-vaccinated horses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5825116/ https://www.ncbi.nlm.nih.gov/pubmed/29474474 http://dx.doi.org/10.1371/journal.pone.0193424 |
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