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16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters

Photoperiod is an important factor in stimulating broiler performance in commercial poultry practice. However, the mechanism by which photoperiod affects the performance of broiler chickens has not been adequately explored. The current study evaluated the effects of three different photoperiod regim...

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Autores principales: Wang, Jun, Nesengani, Lucky T., Gong, Yongsheng, Yang, Yujiang, Lu, Wenfa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5825889/
https://www.ncbi.nlm.nih.gov/pubmed/29492337
http://dx.doi.org/10.7717/peerj.4390
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author Wang, Jun
Nesengani, Lucky T.
Gong, Yongsheng
Yang, Yujiang
Lu, Wenfa
author_facet Wang, Jun
Nesengani, Lucky T.
Gong, Yongsheng
Yang, Yujiang
Lu, Wenfa
author_sort Wang, Jun
collection PubMed
description Photoperiod is an important factor in stimulating broiler performance in commercial poultry practice. However, the mechanism by which photoperiod affects the performance of broiler chickens has not been adequately explored. The current study evaluated the effects of three different photoperiod regimes (short day (LD) = 8 h light, control (CTR) = 12.5 h light, and long day (SD) = 16 h light) on the cecal microbiota of broiler roosters by sequencing bacterial 16S rRNA genes. At the phylum level, the dominant bacteria were Firmicutes (CTR: 68%, SD: 69%, LD: 67%) and Bacteroidetes (CTR: 25%, SD: 26%, and LD: 28%). There was a greater abundance of Proteobacteria (p < 0.01) and Cyanobacteria (p < 0.05) in chickens in the LD group than in those in the CTR group. A significantly greater abundance of Actinobacteria was observed in CTR chickens than in SD and LD chickens (p < 0.01). The abundance of Deferribacteres was significantly higher in LD chickens than in SD chickens (p < 0.01). Fusobacteria and Proteobacteria were more abundant in SD chickens than in CTR and LD chickens. The predicted functional properties indicate that cellular processes may be influenced by photoperiod. Conversely, carbohydrate metabolism was enhanced in CTR chickens as compared to that in SD and LD chickens. The current results indicate that different photoperiod regimes may influence the abundance of specific bacterial populations and then contribute to differences in the functional properties of gut microbiota of broiler roosters.
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spelling pubmed-58258892018-02-28 16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters Wang, Jun Nesengani, Lucky T. Gong, Yongsheng Yang, Yujiang Lu, Wenfa PeerJ Agricultural Science Photoperiod is an important factor in stimulating broiler performance in commercial poultry practice. However, the mechanism by which photoperiod affects the performance of broiler chickens has not been adequately explored. The current study evaluated the effects of three different photoperiod regimes (short day (LD) = 8 h light, control (CTR) = 12.5 h light, and long day (SD) = 16 h light) on the cecal microbiota of broiler roosters by sequencing bacterial 16S rRNA genes. At the phylum level, the dominant bacteria were Firmicutes (CTR: 68%, SD: 69%, LD: 67%) and Bacteroidetes (CTR: 25%, SD: 26%, and LD: 28%). There was a greater abundance of Proteobacteria (p < 0.01) and Cyanobacteria (p < 0.05) in chickens in the LD group than in those in the CTR group. A significantly greater abundance of Actinobacteria was observed in CTR chickens than in SD and LD chickens (p < 0.01). The abundance of Deferribacteres was significantly higher in LD chickens than in SD chickens (p < 0.01). Fusobacteria and Proteobacteria were more abundant in SD chickens than in CTR and LD chickens. The predicted functional properties indicate that cellular processes may be influenced by photoperiod. Conversely, carbohydrate metabolism was enhanced in CTR chickens as compared to that in SD and LD chickens. The current results indicate that different photoperiod regimes may influence the abundance of specific bacterial populations and then contribute to differences in the functional properties of gut microbiota of broiler roosters. PeerJ Inc. 2018-02-22 /pmc/articles/PMC5825889/ /pubmed/29492337 http://dx.doi.org/10.7717/peerj.4390 Text en ©2018 Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Wang, Jun
Nesengani, Lucky T.
Gong, Yongsheng
Yang, Yujiang
Lu, Wenfa
16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters
title 16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters
title_full 16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters
title_fullStr 16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters
title_full_unstemmed 16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters
title_short 16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters
title_sort 16s rrna gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5825889/
https://www.ncbi.nlm.nih.gov/pubmed/29492337
http://dx.doi.org/10.7717/peerj.4390
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