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Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran
Background: The presence of Class 1, 2 and 3 integrons in clinical isolates of Pseudomonas aeruginosa with multi-drug resistance phenotype has rendered the organism as a new concern. Objective: This study aimed to investigate the prevalence of Class 1, 2 and 3 integrons in multi-drug resistant clini...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5825915/ https://www.ncbi.nlm.nih.gov/pubmed/29480797 http://dx.doi.org/10.1051/bmdcn/2018080102 |
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author | Mobaraki, Shahram Aghazadeh, Mohammad Soroush Barhaghi, Mohammad Hossein Yousef Memar, Mohammad Goli, Hamid Reza Gholizadeh, Pourya Samadi Kafil, Hossein |
author_facet | Mobaraki, Shahram Aghazadeh, Mohammad Soroush Barhaghi, Mohammad Hossein Yousef Memar, Mohammad Goli, Hamid Reza Gholizadeh, Pourya Samadi Kafil, Hossein |
author_sort | Mobaraki, Shahram |
collection | PubMed |
description | Background: The presence of Class 1, 2 and 3 integrons in clinical isolates of Pseudomonas aeruginosa with multi-drug resistance phenotype has rendered the organism as a new concern. Objective: This study aimed to investigate the prevalence of Class 1, 2 and 3 integrons in multi-drug resistant clinical isolates of Pseudomonas aeruginosa collected from hospitals in the city of Tabriz Materials and Methods: A total of 200 P. aeruginosa non-duplicated clinical isolates were collected from inpatients and outpatients in different wards of hospitals from May to November 2016. The bacteria were identified by conventional microbiological methods. Antibiotic susceptibility test was performed by disk diffusion method and the presence of integrons was analyzed by polymerase chain reaction (PCR). Results: Colistin was the most effective antibiotic, while 98% of the isolates were resistant to cefotaxime. Fifty-three percent of the isolates were recorded as multi-drug resistant (MDR) phenotype; however, 27.5% of the isolates were resistant to more than 8 antibiotics. In this study, 55 (27.5%), 51 (25.5%), and 30 (15%) clinical isolates of P. aeruginosa were positive for Class 1, 2 and 3 integrons, respectively. aac(6)II in Class I integrons and dfrA1 in ClassII and aacA7 in Class II integrons were the most prevalent genes. Resistance to aminoglycosides were the most common genes harbored by integrons. Conclusion: The results of this study showed that the prevalence of Class 1, 2 and 3 in integron genes in most P. aeruginosa strains islated from different parts and equipment used in the hospital. The role of these transferable genetic agents has been proven in the creation of resistance. Therefore, it is essential to use management practices to optimize the use of antibiotics, preferably based on the results of antibiogram and trace coding genes for antibiotic resistance. |
format | Online Article Text |
id | pubmed-5825915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-58259152018-02-28 Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran Mobaraki, Shahram Aghazadeh, Mohammad Soroush Barhaghi, Mohammad Hossein Yousef Memar, Mohammad Goli, Hamid Reza Gholizadeh, Pourya Samadi Kafil, Hossein Biomedicine (Taipei) Original Article Background: The presence of Class 1, 2 and 3 integrons in clinical isolates of Pseudomonas aeruginosa with multi-drug resistance phenotype has rendered the organism as a new concern. Objective: This study aimed to investigate the prevalence of Class 1, 2 and 3 integrons in multi-drug resistant clinical isolates of Pseudomonas aeruginosa collected from hospitals in the city of Tabriz Materials and Methods: A total of 200 P. aeruginosa non-duplicated clinical isolates were collected from inpatients and outpatients in different wards of hospitals from May to November 2016. The bacteria were identified by conventional microbiological methods. Antibiotic susceptibility test was performed by disk diffusion method and the presence of integrons was analyzed by polymerase chain reaction (PCR). Results: Colistin was the most effective antibiotic, while 98% of the isolates were resistant to cefotaxime. Fifty-three percent of the isolates were recorded as multi-drug resistant (MDR) phenotype; however, 27.5% of the isolates were resistant to more than 8 antibiotics. In this study, 55 (27.5%), 51 (25.5%), and 30 (15%) clinical isolates of P. aeruginosa were positive for Class 1, 2 and 3 integrons, respectively. aac(6)II in Class I integrons and dfrA1 in ClassII and aacA7 in Class II integrons were the most prevalent genes. Resistance to aminoglycosides were the most common genes harbored by integrons. Conclusion: The results of this study showed that the prevalence of Class 1, 2 and 3 in integron genes in most P. aeruginosa strains islated from different parts and equipment used in the hospital. The role of these transferable genetic agents has been proven in the creation of resistance. Therefore, it is essential to use management practices to optimize the use of antibiotics, preferably based on the results of antibiogram and trace coding genes for antibiotic resistance. EDP Sciences 2018-02-26 /pmc/articles/PMC5825915/ /pubmed/29480797 http://dx.doi.org/10.1051/bmdcn/2018080102 Text en © Author(s) 2018. This article is published with open access by China Medical University Open Access This article is distributed under terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) which permits any use, distribution, and reproduction in any medium, provided original author(s) and source are credited. |
spellingShingle | Original Article Mobaraki, Shahram Aghazadeh, Mohammad Soroush Barhaghi, Mohammad Hossein Yousef Memar, Mohammad Goli, Hamid Reza Gholizadeh, Pourya Samadi Kafil, Hossein Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran |
title | Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran |
title_full | Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran |
title_fullStr | Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran |
title_full_unstemmed | Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran |
title_short | Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran |
title_sort | prevalence of integrons 1, 2, 3 associated with antibiotic resistance in pseudomonas aeruginosa isolates from northwest of iran |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5825915/ https://www.ncbi.nlm.nih.gov/pubmed/29480797 http://dx.doi.org/10.1051/bmdcn/2018080102 |
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