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Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models
Staphylococcus aureus produces membrane-derived vesicles (MVs), which share functional properties to outer membrane vesicles. Atomic force microscopy revealed that S. aureus-derived MVs are associated with the bacterial surface or released into the surrounding environment depending on bacterial grow...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5826277/ https://www.ncbi.nlm.nih.gov/pubmed/29515544 http://dx.doi.org/10.3389/fmicb.2018.00262 |
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author | Askarian, Fatemeh Lapek, John D. Dongre, Mitesh Tsai, Chih-Ming Kumaraswamy, Monika Kousha, Armin Valderrama, J. Andrés Ludviksen, Judith A. Cavanagh, Jorunn P. Uchiyama, Satoshi Mollnes, Tom E. Gonzalez, David J. Wai, Sun N. Nizet, Victor Johannessen, Mona |
author_facet | Askarian, Fatemeh Lapek, John D. Dongre, Mitesh Tsai, Chih-Ming Kumaraswamy, Monika Kousha, Armin Valderrama, J. Andrés Ludviksen, Judith A. Cavanagh, Jorunn P. Uchiyama, Satoshi Mollnes, Tom E. Gonzalez, David J. Wai, Sun N. Nizet, Victor Johannessen, Mona |
author_sort | Askarian, Fatemeh |
collection | PubMed |
description | Staphylococcus aureus produces membrane-derived vesicles (MVs), which share functional properties to outer membrane vesicles. Atomic force microscopy revealed that S. aureus-derived MVs are associated with the bacterial surface or released into the surrounding environment depending on bacterial growth conditions. By using a comparative proteomic approach, a total of 131 and 617 proteins were identified in MVs isolated from S. aureus grown in Luria-Bertani and brain-heart infusion broth, respectively. Purified S. aureus MVs derived from the bacteria grown in either media induced comparable levels of cytotoxicity and neutrophil-activation. Administration of exogenous MVs increased the resistance of S. aureus to killing by whole blood or purified human neutrophils ex vivo and increased S. aureus survival in vivo. Finally, immunization of mice with S. aureus-derived MVs induced production of IgM, total IgG, IgG1, IgG2a, and IgG2b resulting in protection against subcutaneous and systemic S. aureus infection. Collectively, our results suggest S. aureus MVs can influence bacterial–host interactions during systemic infections and provide protective immunity in murine models of infection. |
format | Online Article Text |
id | pubmed-5826277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58262772018-03-07 Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models Askarian, Fatemeh Lapek, John D. Dongre, Mitesh Tsai, Chih-Ming Kumaraswamy, Monika Kousha, Armin Valderrama, J. Andrés Ludviksen, Judith A. Cavanagh, Jorunn P. Uchiyama, Satoshi Mollnes, Tom E. Gonzalez, David J. Wai, Sun N. Nizet, Victor Johannessen, Mona Front Microbiol Microbiology Staphylococcus aureus produces membrane-derived vesicles (MVs), which share functional properties to outer membrane vesicles. Atomic force microscopy revealed that S. aureus-derived MVs are associated with the bacterial surface or released into the surrounding environment depending on bacterial growth conditions. By using a comparative proteomic approach, a total of 131 and 617 proteins were identified in MVs isolated from S. aureus grown in Luria-Bertani and brain-heart infusion broth, respectively. Purified S. aureus MVs derived from the bacteria grown in either media induced comparable levels of cytotoxicity and neutrophil-activation. Administration of exogenous MVs increased the resistance of S. aureus to killing by whole blood or purified human neutrophils ex vivo and increased S. aureus survival in vivo. Finally, immunization of mice with S. aureus-derived MVs induced production of IgM, total IgG, IgG1, IgG2a, and IgG2b resulting in protection against subcutaneous and systemic S. aureus infection. Collectively, our results suggest S. aureus MVs can influence bacterial–host interactions during systemic infections and provide protective immunity in murine models of infection. Frontiers Media S.A. 2018-02-20 /pmc/articles/PMC5826277/ /pubmed/29515544 http://dx.doi.org/10.3389/fmicb.2018.00262 Text en Copyright © 2018 Askarian, Lapek, Dongre, Tsai, Kumaraswamy, Kousha, Valderrama, Ludviksen, Cavanagh, Uchiyama, Mollnes, Gonzalez, Wai, Nizet and Johannessen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Askarian, Fatemeh Lapek, John D. Dongre, Mitesh Tsai, Chih-Ming Kumaraswamy, Monika Kousha, Armin Valderrama, J. Andrés Ludviksen, Judith A. Cavanagh, Jorunn P. Uchiyama, Satoshi Mollnes, Tom E. Gonzalez, David J. Wai, Sun N. Nizet, Victor Johannessen, Mona Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models |
title | Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models |
title_full | Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models |
title_fullStr | Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models |
title_full_unstemmed | Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models |
title_short | Staphylococcus aureus Membrane-Derived Vesicles Promote Bacterial Virulence and Confer Protective Immunity in Murine Infection Models |
title_sort | staphylococcus aureus membrane-derived vesicles promote bacterial virulence and confer protective immunity in murine infection models |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5826277/ https://www.ncbi.nlm.nih.gov/pubmed/29515544 http://dx.doi.org/10.3389/fmicb.2018.00262 |
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