Cargando…
In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability
Stem cell migration and interaction with pathology are critical to understand the complexity and status of disease recovery progress. However, the dynamic visualization still remains a great challenge due to imaging technical limitation, cell labeling difficulty, or blood–brain barrier (BBB). Herein...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827566/ https://www.ncbi.nlm.nih.gov/pubmed/29619293 http://dx.doi.org/10.1002/advs.201700277 |
_version_ | 1783302498390376448 |
---|---|
author | Li, Weitao Chen, Ronghe Lv, Jing Wang, Hongke Liu, Yu Peng, Ya Qian, Zhiyu Fu, Guo Nie, Liming |
author_facet | Li, Weitao Chen, Ronghe Lv, Jing Wang, Hongke Liu, Yu Peng, Ya Qian, Zhiyu Fu, Guo Nie, Liming |
author_sort | Li, Weitao |
collection | PubMed |
description | Stem cell migration and interaction with pathology are critical to understand the complexity and status of disease recovery progress. However, the dynamic visualization still remains a great challenge due to imaging technical limitation, cell labeling difficulty, or blood–brain barrier (BBB). Herein, fast photoacoustic tomography (PAT) with optical molecular probes is applied to noninvasively monitor traumatic brain injury (TBI) and its rehabilitation. The vascular distribution and TBI hemorrhage are clearly imaged, longitudinally monitored, and quantified. Bone mesenchymal stem cells (BMSCs) labeled with modified Prussian blue particles (PBPs), excellent near‐infrared dyes and photoacoustic contrasts, are intravenously injected to the mice for improved observation and efficient therapy. BMSCs are demonstrated to be capable of overcoming BBB with enhanced delivery of PBPs to the brain parenchyma. Notably, the versatile BMSCs are observed by PAT to home to the damage region and repair the ruptured vasculature. Moreover, the wound treated by BMSCs exhibits much faster recovery speed than that without treatment. These findings can potentially provide a new noninvasive and high‐resolution approach to image TBI, monitor recovery process, and especially trace BMSCs. This study will stimulate extensive researches on brain diseases and provide promising strategies of dye labeled BMSCs in regenerative medicine. |
format | Online Article Text |
id | pubmed-5827566 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58275662018-04-04 In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability Li, Weitao Chen, Ronghe Lv, Jing Wang, Hongke Liu, Yu Peng, Ya Qian, Zhiyu Fu, Guo Nie, Liming Adv Sci (Weinh) Full Papers Stem cell migration and interaction with pathology are critical to understand the complexity and status of disease recovery progress. However, the dynamic visualization still remains a great challenge due to imaging technical limitation, cell labeling difficulty, or blood–brain barrier (BBB). Herein, fast photoacoustic tomography (PAT) with optical molecular probes is applied to noninvasively monitor traumatic brain injury (TBI) and its rehabilitation. The vascular distribution and TBI hemorrhage are clearly imaged, longitudinally monitored, and quantified. Bone mesenchymal stem cells (BMSCs) labeled with modified Prussian blue particles (PBPs), excellent near‐infrared dyes and photoacoustic contrasts, are intravenously injected to the mice for improved observation and efficient therapy. BMSCs are demonstrated to be capable of overcoming BBB with enhanced delivery of PBPs to the brain parenchyma. Notably, the versatile BMSCs are observed by PAT to home to the damage region and repair the ruptured vasculature. Moreover, the wound treated by BMSCs exhibits much faster recovery speed than that without treatment. These findings can potentially provide a new noninvasive and high‐resolution approach to image TBI, monitor recovery process, and especially trace BMSCs. This study will stimulate extensive researches on brain diseases and provide promising strategies of dye labeled BMSCs in regenerative medicine. John Wiley and Sons Inc. 2017-12-05 /pmc/articles/PMC5827566/ /pubmed/29619293 http://dx.doi.org/10.1002/advs.201700277 Text en © 2017 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Li, Weitao Chen, Ronghe Lv, Jing Wang, Hongke Liu, Yu Peng, Ya Qian, Zhiyu Fu, Guo Nie, Liming In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability |
title | In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability |
title_full | In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability |
title_fullStr | In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability |
title_full_unstemmed | In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability |
title_short | In Vivo Photoacoustic Imaging of Brain Injury and Rehabilitation by High‐Efficient Near‐Infrared Dye Labeled Mesenchymal Stem Cells with Enhanced Brain Barrier Permeability |
title_sort | in vivo photoacoustic imaging of brain injury and rehabilitation by high‐efficient near‐infrared dye labeled mesenchymal stem cells with enhanced brain barrier permeability |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827566/ https://www.ncbi.nlm.nih.gov/pubmed/29619293 http://dx.doi.org/10.1002/advs.201700277 |
work_keys_str_mv | AT liweitao invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability AT chenronghe invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability AT lvjing invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability AT wanghongke invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability AT liuyu invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability AT pengya invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability AT qianzhiyu invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability AT fuguo invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability AT nieliming invivophotoacousticimagingofbraininjuryandrehabilitationbyhighefficientnearinfrareddyelabeledmesenchymalstemcellswithenhancedbrainbarrierpermeability |