Cargando…
Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation
Understanding cell–biomaterial interactions is critical for the control of cell fate for tissue engineering and regenerative medicine. Here, cerium oxide nanoparticles (CeONPs) are applied at different Ce(4+)/Ce(3+) ratios (i.e., 0.46, 1.23, and 3.23) to titanium substrate surfaces by magnetron sput...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827567/ https://www.ncbi.nlm.nih.gov/pubmed/29610729 http://dx.doi.org/10.1002/advs.201700678 |
_version_ | 1783302498632597504 |
---|---|
author | Li, Jinhua Wen, Jin Li, Bin Li, Wan Qiao, Wei Shen, Jie Jin, Weihong Jiang, Xinquan Yeung, Kelvin W. K. Chu, Paul K. |
author_facet | Li, Jinhua Wen, Jin Li, Bin Li, Wan Qiao, Wei Shen, Jie Jin, Weihong Jiang, Xinquan Yeung, Kelvin W. K. Chu, Paul K. |
author_sort | Li, Jinhua |
collection | PubMed |
description | Understanding cell–biomaterial interactions is critical for the control of cell fate for tissue engineering and regenerative medicine. Here, cerium oxide nanoparticles (CeONPs) are applied at different Ce(4+)/Ce(3+) ratios (i.e., 0.46, 1.23, and 3.23) to titanium substrate surfaces by magnetron sputtering and vacuum annealing. Evaluation of the cytotoxicity of the modified surface to cultured rat bone marrow mesenchymal stem cells (BMSCs) reveals that the cytocompatibility and cell proliferation are proportional to the increases in Ce(4+)/Ce(3+) ratio on titanium surface. The bone formation capability induced by these surface modified titanium alloys is evaluated by implanting various CeONP samples into the intramedullary cavity of rat femur for 8 weeks. New bone formation adjacent to the implant shows a close relationship to the surface Ce(4+)/Ce(3+) ratio; higher Ce(4+)/Ce(3+) ratio achieves better osseointegration. The mechanism of this in vivo outcome is explored by culturing rat BMSCs and RAW264.7 murine macrophages on CeONP samples for different durations. The improvement in osteogenic differentiation capability of BMSCs is directly proportional to the increased Ce(4+)/Ce(3+) ratio on the titanium surface. Increases in the Ce(4+)/Ce(3+) ratio also elevate the polarization of the M2 phenotype of RAW264.7 murine macrophages, particularly with respect to the healing‐associated M2 percentage and anti‐inflammatory cytokine secretion. The manipulation of valence states of CeONPs appears to provide an effective modulation of the osteogenic capability of stem cells and the M2 polarization of macrophages, resulting in favorable outcomes of new bone formation and osseointegration. |
format | Online Article Text |
id | pubmed-5827567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58275672018-04-02 Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation Li, Jinhua Wen, Jin Li, Bin Li, Wan Qiao, Wei Shen, Jie Jin, Weihong Jiang, Xinquan Yeung, Kelvin W. K. Chu, Paul K. Adv Sci (Weinh) Full Papers Understanding cell–biomaterial interactions is critical for the control of cell fate for tissue engineering and regenerative medicine. Here, cerium oxide nanoparticles (CeONPs) are applied at different Ce(4+)/Ce(3+) ratios (i.e., 0.46, 1.23, and 3.23) to titanium substrate surfaces by magnetron sputtering and vacuum annealing. Evaluation of the cytotoxicity of the modified surface to cultured rat bone marrow mesenchymal stem cells (BMSCs) reveals that the cytocompatibility and cell proliferation are proportional to the increases in Ce(4+)/Ce(3+) ratio on titanium surface. The bone formation capability induced by these surface modified titanium alloys is evaluated by implanting various CeONP samples into the intramedullary cavity of rat femur for 8 weeks. New bone formation adjacent to the implant shows a close relationship to the surface Ce(4+)/Ce(3+) ratio; higher Ce(4+)/Ce(3+) ratio achieves better osseointegration. The mechanism of this in vivo outcome is explored by culturing rat BMSCs and RAW264.7 murine macrophages on CeONP samples for different durations. The improvement in osteogenic differentiation capability of BMSCs is directly proportional to the increased Ce(4+)/Ce(3+) ratio on the titanium surface. Increases in the Ce(4+)/Ce(3+) ratio also elevate the polarization of the M2 phenotype of RAW264.7 murine macrophages, particularly with respect to the healing‐associated M2 percentage and anti‐inflammatory cytokine secretion. The manipulation of valence states of CeONPs appears to provide an effective modulation of the osteogenic capability of stem cells and the M2 polarization of macrophages, resulting in favorable outcomes of new bone formation and osseointegration. John Wiley and Sons Inc. 2017-12-18 /pmc/articles/PMC5827567/ /pubmed/29610729 http://dx.doi.org/10.1002/advs.201700678 Text en © 2017 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Li, Jinhua Wen, Jin Li, Bin Li, Wan Qiao, Wei Shen, Jie Jin, Weihong Jiang, Xinquan Yeung, Kelvin W. K. Chu, Paul K. Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation |
title | Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation |
title_full | Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation |
title_fullStr | Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation |
title_full_unstemmed | Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation |
title_short | Valence State Manipulation of Cerium Oxide Nanoparticles on a Titanium Surface for Modulating Cell Fate and Bone Formation |
title_sort | valence state manipulation of cerium oxide nanoparticles on a titanium surface for modulating cell fate and bone formation |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827567/ https://www.ncbi.nlm.nih.gov/pubmed/29610729 http://dx.doi.org/10.1002/advs.201700678 |
work_keys_str_mv | AT lijinhua valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT wenjin valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT libin valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT liwan valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT qiaowei valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT shenjie valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT jinweihong valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT jiangxinquan valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT yeungkelvinwk valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation AT chupaulk valencestatemanipulationofceriumoxidenanoparticlesonatitaniumsurfaceformodulatingcellfateandboneformation |