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Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation
Chlamydia trachomatis (Ctr) is a bacterial pathogen that causes ocular, urogenital and lymph system infections in humans. It is highly abundant and among its serovars, E, F and D are most prevalent in sexually transmitted disease. However, the number of publicly available genome sequences of the ser...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827700/ https://www.ncbi.nlm.nih.gov/pubmed/29186396 http://dx.doi.org/10.1093/femspd/ftx120 |
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author | Eder, Thomas Kobus, Stefanie Stallmann, Sonja Stepanow, Stefanie Köhrer, Karl Hegemann, Johannes H Rattei, Thomas |
author_facet | Eder, Thomas Kobus, Stefanie Stallmann, Sonja Stepanow, Stefanie Köhrer, Karl Hegemann, Johannes H Rattei, Thomas |
author_sort | Eder, Thomas |
collection | PubMed |
description | Chlamydia trachomatis (Ctr) is a bacterial pathogen that causes ocular, urogenital and lymph system infections in humans. It is highly abundant and among its serovars, E, F and D are most prevalent in sexually transmitted disease. However, the number of publicly available genome sequences of the serovars E and F, and thereby our knowledge about the molecular architecture of these serovars, is low. Here we sequenced the genomes of six E and F clinical isolates and one E lab strain, in order to study the genetic variance in these serovars. As observed before, the genomic variation inside the Ctr genomes is very low and the phylogenetic placement in comparison to publicly available genomes is as expected by ompA gene serotyping. However, we observed a large InDel carrying four to five open reading frames in one clinical E sample and in the E lab strain. We have also observed substantial variation on nucleotide and amino acid levels, especially in membrane proteins and secreted proteins. Furthermore, these two groups of proteins are also target for recombination events. One clinical F isolate was genetically heterogeneous and revealed the highest differences on nucleotide level in the pmpE gene. |
format | Online Article Text |
id | pubmed-5827700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58277002018-03-05 Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation Eder, Thomas Kobus, Stefanie Stallmann, Sonja Stepanow, Stefanie Köhrer, Karl Hegemann, Johannes H Rattei, Thomas Pathog Dis Shortomics Chlamydia trachomatis (Ctr) is a bacterial pathogen that causes ocular, urogenital and lymph system infections in humans. It is highly abundant and among its serovars, E, F and D are most prevalent in sexually transmitted disease. However, the number of publicly available genome sequences of the serovars E and F, and thereby our knowledge about the molecular architecture of these serovars, is low. Here we sequenced the genomes of six E and F clinical isolates and one E lab strain, in order to study the genetic variance in these serovars. As observed before, the genomic variation inside the Ctr genomes is very low and the phylogenetic placement in comparison to publicly available genomes is as expected by ompA gene serotyping. However, we observed a large InDel carrying four to five open reading frames in one clinical E sample and in the E lab strain. We have also observed substantial variation on nucleotide and amino acid levels, especially in membrane proteins and secreted proteins. Furthermore, these two groups of proteins are also target for recombination events. One clinical F isolate was genetically heterogeneous and revealed the highest differences on nucleotide level in the pmpE gene. Oxford University Press 2017-11-24 /pmc/articles/PMC5827700/ /pubmed/29186396 http://dx.doi.org/10.1093/femspd/ftx120 Text en © FEMS 2017. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Shortomics Eder, Thomas Kobus, Stefanie Stallmann, Sonja Stepanow, Stefanie Köhrer, Karl Hegemann, Johannes H Rattei, Thomas Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation |
title | Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation |
title_full | Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation |
title_fullStr | Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation |
title_full_unstemmed | Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation |
title_short | Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variation |
title_sort | genome sequencing of chlamydia trachomatis serovars e and f reveals substantial genetic variation |
topic | Shortomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827700/ https://www.ncbi.nlm.nih.gov/pubmed/29186396 http://dx.doi.org/10.1093/femspd/ftx120 |
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