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Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma

Tumour-derived exosomes (TEX) are a subset of extracellular vesicles (EVs) present in body fluids of patients with cancer. The role of this exosome subset in melanoma progression has been of interest ever since ex vivo studies of exosomes produced by melanoma cell lines were shown to suppress anti-m...

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Autores principales: Sharma, Priyanka, Ludwig, Sonja, Muller, Laurent, Hong, Chang Sook, Kirkwood, John M, Ferrone, Soldano, Whiteside, Theresa L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827723/
https://www.ncbi.nlm.nih.gov/pubmed/29511460
http://dx.doi.org/10.1080/20013078.2018.1435138
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author Sharma, Priyanka
Ludwig, Sonja
Muller, Laurent
Hong, Chang Sook
Kirkwood, John M
Ferrone, Soldano
Whiteside, Theresa L.
author_facet Sharma, Priyanka
Ludwig, Sonja
Muller, Laurent
Hong, Chang Sook
Kirkwood, John M
Ferrone, Soldano
Whiteside, Theresa L.
author_sort Sharma, Priyanka
collection PubMed
description Tumour-derived exosomes (TEX) are a subset of extracellular vesicles (EVs) present in body fluids of patients with cancer. The role of this exosome subset in melanoma progression has been of interest ever since ex vivo studies of exosomes produced by melanoma cell lines were shown to suppress anti-melanoma immune responses. To study the impact of melanoma-derived exosomes (MTEX) present in patients’ plasma on melanoma progression, isolation of MTEX from total plasma exosomes is necessary. We have developed an immunoaffinity-based method for MTEX capture from plasma of melanoma patients. Using mAb 763.74 specific for the CSPG4 epitope uniquely expressed on melanoma cells, we separated MTEX from non-tumour cell-derived exosomes and evaluated the protein cargo of both fractions by quantitative flow cytometry. Melanoma-associated antigens were carried by MTEX but were not detectable in exosomes produced by normal cells. Separation of plasma-derived MTEX from non-MTEX provides an opportunity for future evaluation of MTEX as potential biomarkers of melanoma progression and as surrogates of melanoma in tumour liquid biopsy studies.
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spelling pubmed-58277232018-03-06 Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma Sharma, Priyanka Ludwig, Sonja Muller, Laurent Hong, Chang Sook Kirkwood, John M Ferrone, Soldano Whiteside, Theresa L. J Extracell Vesicles Research Article Tumour-derived exosomes (TEX) are a subset of extracellular vesicles (EVs) present in body fluids of patients with cancer. The role of this exosome subset in melanoma progression has been of interest ever since ex vivo studies of exosomes produced by melanoma cell lines were shown to suppress anti-melanoma immune responses. To study the impact of melanoma-derived exosomes (MTEX) present in patients’ plasma on melanoma progression, isolation of MTEX from total plasma exosomes is necessary. We have developed an immunoaffinity-based method for MTEX capture from plasma of melanoma patients. Using mAb 763.74 specific for the CSPG4 epitope uniquely expressed on melanoma cells, we separated MTEX from non-tumour cell-derived exosomes and evaluated the protein cargo of both fractions by quantitative flow cytometry. Melanoma-associated antigens were carried by MTEX but were not detectable in exosomes produced by normal cells. Separation of plasma-derived MTEX from non-MTEX provides an opportunity for future evaluation of MTEX as potential biomarkers of melanoma progression and as surrogates of melanoma in tumour liquid biopsy studies. Taylor & Francis 2018-02-15 /pmc/articles/PMC5827723/ /pubmed/29511460 http://dx.doi.org/10.1080/20013078.2018.1435138 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sharma, Priyanka
Ludwig, Sonja
Muller, Laurent
Hong, Chang Sook
Kirkwood, John M
Ferrone, Soldano
Whiteside, Theresa L.
Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma
title Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma
title_full Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma
title_fullStr Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma
title_full_unstemmed Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma
title_short Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma
title_sort immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827723/
https://www.ncbi.nlm.nih.gov/pubmed/29511460
http://dx.doi.org/10.1080/20013078.2018.1435138
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