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A rice class-XIV kinesin enters the nucleus in response to cold
Higher plants possess a large number of kinesins, but lack the minus-end directed dynein motors. However, the kinesin class XIV has strongly expanded, and minus-end directed motors from this class may have taken over functions of cytoplasmic dyneins. In this study, we address the functional aspects...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827730/ https://www.ncbi.nlm.nih.gov/pubmed/29483672 http://dx.doi.org/10.1038/s41598-018-21816-w |
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author | Xu, Xiaolu Walter, Wilhelm J. Liu, Qiong Machens, Isabel Nick, Peter |
author_facet | Xu, Xiaolu Walter, Wilhelm J. Liu, Qiong Machens, Isabel Nick, Peter |
author_sort | Xu, Xiaolu |
collection | PubMed |
description | Higher plants possess a large number of kinesins, but lack the minus-end directed dynein motors. However, the kinesin class XIV has strongly expanded, and minus-end directed motors from this class may have taken over functions of cytoplasmic dyneins. In this study, we address the functional aspects of a novel rice homologue of the Arabidopsis class-XIV kinesins ATK1 and ATK5. Since a loss-of-function rice mutant of this kinesin is not viable, the function was studied in tobacco BY-2 as heterologous system. OsDLK-GFP stably expressed in BY-2 cells decorates cortical microtubules, but also can shift into the nucleus of interphase cells. Because of this peculiar localisation, we coined the name Dual Localisation Kinesin (DLK). The nuclear import of this protein is strongly and reversibly promoted in response to cold. During mitosis, OsDLK is repartitioned between spindle and phragmoplast. Motility assays in vitro using show that OsDLK can convey mutual sliding of microtubules and moves at a velocity comparable to other class-XIV kinesins. When tobacco cells overexpressing OsDLK are synchronised, they exhibit a delayed entry into metaphase, while the later phases of mitosis are accelerated. The data are discussed in relation to additional functions of this kinesin type, beyond their transport along microtubules. |
format | Online Article Text |
id | pubmed-5827730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58277302018-03-01 A rice class-XIV kinesin enters the nucleus in response to cold Xu, Xiaolu Walter, Wilhelm J. Liu, Qiong Machens, Isabel Nick, Peter Sci Rep Article Higher plants possess a large number of kinesins, but lack the minus-end directed dynein motors. However, the kinesin class XIV has strongly expanded, and minus-end directed motors from this class may have taken over functions of cytoplasmic dyneins. In this study, we address the functional aspects of a novel rice homologue of the Arabidopsis class-XIV kinesins ATK1 and ATK5. Since a loss-of-function rice mutant of this kinesin is not viable, the function was studied in tobacco BY-2 as heterologous system. OsDLK-GFP stably expressed in BY-2 cells decorates cortical microtubules, but also can shift into the nucleus of interphase cells. Because of this peculiar localisation, we coined the name Dual Localisation Kinesin (DLK). The nuclear import of this protein is strongly and reversibly promoted in response to cold. During mitosis, OsDLK is repartitioned between spindle and phragmoplast. Motility assays in vitro using show that OsDLK can convey mutual sliding of microtubules and moves at a velocity comparable to other class-XIV kinesins. When tobacco cells overexpressing OsDLK are synchronised, they exhibit a delayed entry into metaphase, while the later phases of mitosis are accelerated. The data are discussed in relation to additional functions of this kinesin type, beyond their transport along microtubules. Nature Publishing Group UK 2018-02-26 /pmc/articles/PMC5827730/ /pubmed/29483672 http://dx.doi.org/10.1038/s41598-018-21816-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Xu, Xiaolu Walter, Wilhelm J. Liu, Qiong Machens, Isabel Nick, Peter A rice class-XIV kinesin enters the nucleus in response to cold |
title | A rice class-XIV kinesin enters the nucleus in response to cold |
title_full | A rice class-XIV kinesin enters the nucleus in response to cold |
title_fullStr | A rice class-XIV kinesin enters the nucleus in response to cold |
title_full_unstemmed | A rice class-XIV kinesin enters the nucleus in response to cold |
title_short | A rice class-XIV kinesin enters the nucleus in response to cold |
title_sort | rice class-xiv kinesin enters the nucleus in response to cold |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827730/ https://www.ncbi.nlm.nih.gov/pubmed/29483672 http://dx.doi.org/10.1038/s41598-018-21816-w |
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