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A rice class-XIV kinesin enters the nucleus in response to cold

Higher plants possess a large number of kinesins, but lack the minus-end directed dynein motors. However, the kinesin class XIV has strongly expanded, and minus-end directed motors from this class may have taken over functions of cytoplasmic dyneins. In this study, we address the functional aspects...

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Autores principales: Xu, Xiaolu, Walter, Wilhelm J., Liu, Qiong, Machens, Isabel, Nick, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827730/
https://www.ncbi.nlm.nih.gov/pubmed/29483672
http://dx.doi.org/10.1038/s41598-018-21816-w
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author Xu, Xiaolu
Walter, Wilhelm J.
Liu, Qiong
Machens, Isabel
Nick, Peter
author_facet Xu, Xiaolu
Walter, Wilhelm J.
Liu, Qiong
Machens, Isabel
Nick, Peter
author_sort Xu, Xiaolu
collection PubMed
description Higher plants possess a large number of kinesins, but lack the minus-end directed dynein motors. However, the kinesin class XIV has strongly expanded, and minus-end directed motors from this class may have taken over functions of cytoplasmic dyneins. In this study, we address the functional aspects of a novel rice homologue of the Arabidopsis class-XIV kinesins ATK1 and ATK5. Since a loss-of-function rice mutant of this kinesin is not viable, the function was studied in tobacco BY-2 as heterologous system. OsDLK-GFP stably expressed in BY-2 cells decorates cortical microtubules, but also can shift into the nucleus of interphase cells. Because of this peculiar localisation, we coined the name Dual Localisation Kinesin (DLK). The nuclear import of this protein is strongly and reversibly promoted in response to cold. During mitosis, OsDLK is repartitioned between spindle and phragmoplast. Motility assays in vitro using show that OsDLK can convey mutual sliding of microtubules and moves at a velocity comparable to other class-XIV kinesins. When tobacco cells overexpressing OsDLK are synchronised, they exhibit a delayed entry into metaphase, while the later phases of mitosis are accelerated. The data are discussed in relation to additional functions of this kinesin type, beyond their transport along microtubules.
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spelling pubmed-58277302018-03-01 A rice class-XIV kinesin enters the nucleus in response to cold Xu, Xiaolu Walter, Wilhelm J. Liu, Qiong Machens, Isabel Nick, Peter Sci Rep Article Higher plants possess a large number of kinesins, but lack the minus-end directed dynein motors. However, the kinesin class XIV has strongly expanded, and minus-end directed motors from this class may have taken over functions of cytoplasmic dyneins. In this study, we address the functional aspects of a novel rice homologue of the Arabidopsis class-XIV kinesins ATK1 and ATK5. Since a loss-of-function rice mutant of this kinesin is not viable, the function was studied in tobacco BY-2 as heterologous system. OsDLK-GFP stably expressed in BY-2 cells decorates cortical microtubules, but also can shift into the nucleus of interphase cells. Because of this peculiar localisation, we coined the name Dual Localisation Kinesin (DLK). The nuclear import of this protein is strongly and reversibly promoted in response to cold. During mitosis, OsDLK is repartitioned between spindle and phragmoplast. Motility assays in vitro using show that OsDLK can convey mutual sliding of microtubules and moves at a velocity comparable to other class-XIV kinesins. When tobacco cells overexpressing OsDLK are synchronised, they exhibit a delayed entry into metaphase, while the later phases of mitosis are accelerated. The data are discussed in relation to additional functions of this kinesin type, beyond their transport along microtubules. Nature Publishing Group UK 2018-02-26 /pmc/articles/PMC5827730/ /pubmed/29483672 http://dx.doi.org/10.1038/s41598-018-21816-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xu, Xiaolu
Walter, Wilhelm J.
Liu, Qiong
Machens, Isabel
Nick, Peter
A rice class-XIV kinesin enters the nucleus in response to cold
title A rice class-XIV kinesin enters the nucleus in response to cold
title_full A rice class-XIV kinesin enters the nucleus in response to cold
title_fullStr A rice class-XIV kinesin enters the nucleus in response to cold
title_full_unstemmed A rice class-XIV kinesin enters the nucleus in response to cold
title_short A rice class-XIV kinesin enters the nucleus in response to cold
title_sort rice class-xiv kinesin enters the nucleus in response to cold
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5827730/
https://www.ncbi.nlm.nih.gov/pubmed/29483672
http://dx.doi.org/10.1038/s41598-018-21816-w
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