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i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases

We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation. The method generates mouse models containing single-base changes, kilobase-sized deletions, and knock-ins. The...

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Detalles Bibliográficos
Autores principales:	Ohtsuka, Masato, Sato, Masahiro, Miura, Hiromi, Takabayashi, Shuji, Matsuyama, Makoto, Koyano, Takayuki, Arifin, Naomi, Nakamura, Shingo, Wada, Kenta, Gurumurthy, Channabasavaiah B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828090/ https://www.ncbi.nlm.nih.gov/pubmed/29482575 http://dx.doi.org/10.1186/s13059-018-1400-x

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