Cargando…

The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality

BACKGROUND: Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes for sper...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Jane-Fang, Lai, Yu-Hua, Wang, Tse-En, Wei, Yu-Syuan, Chang, Yu-Jia, Li, Sheng-Hsiang, Chin, Shih-Chien, Joshi, Radhika, Chang, Hui-Wen, Tsai, Pei-Shiue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828100/
https://www.ncbi.nlm.nih.gov/pubmed/29482549
http://dx.doi.org/10.1186/s12917-018-1389-0
_version_ 1783302575086370816
author Yu, Jane-Fang
Lai, Yu-Hua
Wang, Tse-En
Wei, Yu-Syuan
Chang, Yu-Jia
Li, Sheng-Hsiang
Chin, Shih-Chien
Joshi, Radhika
Chang, Hui-Wen
Tsai, Pei-Shiue
author_facet Yu, Jane-Fang
Lai, Yu-Hua
Wang, Tse-En
Wei, Yu-Syuan
Chang, Yu-Jia
Li, Sheng-Hsiang
Chin, Shih-Chien
Joshi, Radhika
Chang, Hui-Wen
Tsai, Pei-Shiue
author_sort Yu, Jane-Fang
collection PubMed
description BACKGROUND: Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes for sperm preparation. In this study, we investigated whether collagenase can be used to degelify the semen plug and accelerate the semen liquefaction process in zoo captive chimpanzee species (Pan troglodytes). RESULTS: We showed that incubation of chimpanzee ejaculates with 0.1% type I collagenase efficiently and significantly (p < 0.05) releases 2.7-fold more spermatozoa from the coagulated ejaculates, and this degelification process did not alter sperm morphology or viability; nor did it stimulate spontaneous capacitation or an acrosome reaction as assessed by tyrosine phosphorylation and peanut agglutinin stains; moreover, based on computer assisted sperm analysis assay, motility-related parameters remained similar to those of untreated spermatozoa. When collagenase effects were evaluated on cryopreserved sperm samples, we observed post collagenase treatment in which 2.5% glycerol, as a cryoprotectant, preserved sperm acrosome integrity better than 7.8%; however, 7.8% glycerol, as a cryoprotectant, maintained sperm motility better than that of 2.5% glycerol. CONCLUSIONS: Our results demonstrated for the first time that type I collagenase can be used to obtain a significantly higher number of spermatozoa from colloid chimpanzee semen ejaculate without affecting the physiological properties of spermatozoa, and these results are critical for the subsequent gamete development. Our results would benefit sperm preparation processes and cryopreservation efficiency per ejaculate, as more unaffected spermatozoa can be released from the semen plug within a shorter period of time. These results would also benefit the genetic diversity of the chimpanzee species, using sperm cells from less dominant individuals, and for achieving better pregnancy success in primates with significantly higher amounts of sperm for artificial insemination. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1389-0) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5828100
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-58281002018-02-28 The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality Yu, Jane-Fang Lai, Yu-Hua Wang, Tse-En Wei, Yu-Syuan Chang, Yu-Jia Li, Sheng-Hsiang Chin, Shih-Chien Joshi, Radhika Chang, Hui-Wen Tsai, Pei-Shiue BMC Vet Res Research Article BACKGROUND: Semen from the chimpanzee species becomes a colloidal solid after ejaculation. The formation of this copulatory plug is believed to prevent additional spermatozoa of subsequent mating events from accessing the ova. However, this naturally preserved strategy hampers the processes for sperm preparation. In this study, we investigated whether collagenase can be used to degelify the semen plug and accelerate the semen liquefaction process in zoo captive chimpanzee species (Pan troglodytes). RESULTS: We showed that incubation of chimpanzee ejaculates with 0.1% type I collagenase efficiently and significantly (p < 0.05) releases 2.7-fold more spermatozoa from the coagulated ejaculates, and this degelification process did not alter sperm morphology or viability; nor did it stimulate spontaneous capacitation or an acrosome reaction as assessed by tyrosine phosphorylation and peanut agglutinin stains; moreover, based on computer assisted sperm analysis assay, motility-related parameters remained similar to those of untreated spermatozoa. When collagenase effects were evaluated on cryopreserved sperm samples, we observed post collagenase treatment in which 2.5% glycerol, as a cryoprotectant, preserved sperm acrosome integrity better than 7.8%; however, 7.8% glycerol, as a cryoprotectant, maintained sperm motility better than that of 2.5% glycerol. CONCLUSIONS: Our results demonstrated for the first time that type I collagenase can be used to obtain a significantly higher number of spermatozoa from colloid chimpanzee semen ejaculate without affecting the physiological properties of spermatozoa, and these results are critical for the subsequent gamete development. Our results would benefit sperm preparation processes and cryopreservation efficiency per ejaculate, as more unaffected spermatozoa can be released from the semen plug within a shorter period of time. These results would also benefit the genetic diversity of the chimpanzee species, using sperm cells from less dominant individuals, and for achieving better pregnancy success in primates with significantly higher amounts of sperm for artificial insemination. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1389-0) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-27 /pmc/articles/PMC5828100/ /pubmed/29482549 http://dx.doi.org/10.1186/s12917-018-1389-0 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yu, Jane-Fang
Lai, Yu-Hua
Wang, Tse-En
Wei, Yu-Syuan
Chang, Yu-Jia
Li, Sheng-Hsiang
Chin, Shih-Chien
Joshi, Radhika
Chang, Hui-Wen
Tsai, Pei-Shiue
The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality
title The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality
title_full The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality
title_fullStr The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality
title_full_unstemmed The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality
title_short The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality
title_sort effects of type i collagenase on the degelification of chimpanzee (pan troglodytes) semen plug and sperm quality
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828100/
https://www.ncbi.nlm.nih.gov/pubmed/29482549
http://dx.doi.org/10.1186/s12917-018-1389-0
work_keys_str_mv AT yujanefang theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT laiyuhua theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT wangtseen theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT weiyusyuan theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT changyujia theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT lishenghsiang theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT chinshihchien theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT joshiradhika theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT changhuiwen theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT tsaipeishiue theeffectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT yujanefang effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT laiyuhua effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT wangtseen effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT weiyusyuan effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT changyujia effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT lishenghsiang effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT chinshihchien effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT joshiradhika effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT changhuiwen effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality
AT tsaipeishiue effectsoftypeicollagenaseonthedegelificationofchimpanzeepantroglodytessemenplugandspermquality