Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species

PREMISE OF THE STUDY: We isolated and characterized 16 expressed sequence tag–simple sequence repeat (EST‐SSR) markers in Itea chinensis (Iteaceae), a common evergreen broadleaf tree, for future studies on the genetic diversity and spatial genetic structure of the species. METHODS AND RESULTS: Based...

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Detalles Bibliográficos
Autores principales: Shi, Xianggang, Wu, Haidan, Li, Weixi, Guo, Wuxia, Zheng, Yi, Yu, Shixiao, Huang, Yelin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Primer Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828125/
https://www.ncbi.nlm.nih.gov/pubmed/29732244
http://dx.doi.org/10.1002/aps3.1013
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author Shi, Xianggang
Wu, Haidan
Li, Weixi
Guo, Wuxia
Zheng, Yi
Yu, Shixiao
Huang, Yelin
author_facet Shi, Xianggang
Wu, Haidan
Li, Weixi
Guo, Wuxia
Zheng, Yi
Yu, Shixiao
Huang, Yelin
author_sort Shi, Xianggang
collection PubMed
description PREMISE OF THE STUDY: We isolated and characterized 16 expressed sequence tag–simple sequence repeat (EST‐SSR) markers in Itea chinensis (Iteaceae), a common evergreen broadleaf tree, for future studies on the genetic diversity and spatial genetic structure of the species. METHODS AND RESULTS: Based on transcriptome data of I. chinensis, a total of 36 primer pairs were initially designed and tested. Of these, 16 were successfully amplified and showed clear polymorphism. For these markers, the number of alleles per locus varied from two to 15. The observed and expected heterozygosity ranged from 0 to 0.600 and 0.072 to 0.554, respectively. Furthermore, all loci were successfully cross‐amplified in two congeneric species, I. oblonga and I. yangchunensis. CONCLUSIONS: The EST‐SSR markers described here can be used to study the genetic diversity and phylogeographic patterns of I. chinensis and other related species in Itea.
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spelling pubmed-58281252018-05-04 Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species Shi, Xianggang Wu, Haidan Li, Weixi Guo, Wuxia Zheng, Yi Yu, Shixiao Huang, Yelin Appl Plant Sci Primer Notes PREMISE OF THE STUDY: We isolated and characterized 16 expressed sequence tag–simple sequence repeat (EST‐SSR) markers in Itea chinensis (Iteaceae), a common evergreen broadleaf tree, for future studies on the genetic diversity and spatial genetic structure of the species. METHODS AND RESULTS: Based on transcriptome data of I. chinensis, a total of 36 primer pairs were initially designed and tested. Of these, 16 were successfully amplified and showed clear polymorphism. For these markers, the number of alleles per locus varied from two to 15. The observed and expected heterozygosity ranged from 0 to 0.600 and 0.072 to 0.554, respectively. Furthermore, all loci were successfully cross‐amplified in two congeneric species, I. oblonga and I. yangchunensis. CONCLUSIONS: The EST‐SSR markers described here can be used to study the genetic diversity and phylogeographic patterns of I. chinensis and other related species in Itea. John Wiley and Sons Inc. 2018-02-06 /pmc/articles/PMC5828125/ /pubmed/29732244 http://dx.doi.org/10.1002/aps3.1013 Text en © 2018 Shi et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Primer Notes
Shi, Xianggang
Wu, Haidan
Li, Weixi
Guo, Wuxia
Zheng, Yi
Yu, Shixiao
Huang, Yelin
Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species
title Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species
title_full Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species
title_fullStr Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species
title_full_unstemmed Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species
title_short Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species
title_sort development of polymorphic est‐ssr markers in itea chinensis (iteaceae) and cross‐amplification in related species
topic Primer Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828125/
https://www.ncbi.nlm.nih.gov/pubmed/29732244
http://dx.doi.org/10.1002/aps3.1013
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