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Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis
BACKGROUND: Circular RNAs (circRNAs) are a class of novel RNAs with important biological functions, and aberrant expression of circRNAs has been implicated in human diseases. However, the feasibility of using blood circRNAs as disease biomarkers is largely unknown. METHODS: We explored the potential...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828303/ https://www.ncbi.nlm.nih.gov/pubmed/29248507 http://dx.doi.org/10.1016/j.ebiom.2017.12.007 |
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author | Qian, Zhongqing Liu, Hui Li, Musheng Shi, Junchao Li, Na Zhang, Yao Zhang, Xiaojie Lv, Jingzhu Xie, Xueying Bai, Yunfei Ge, Qinyu Ko, Eun-A Tang, Haiyang Wang, Ting Wang, Xiaojing Wang, Zhaohua Zhou, Tong Gu, Wanjun |
author_facet | Qian, Zhongqing Liu, Hui Li, Musheng Shi, Junchao Li, Na Zhang, Yao Zhang, Xiaojie Lv, Jingzhu Xie, Xueying Bai, Yunfei Ge, Qinyu Ko, Eun-A Tang, Haiyang Wang, Ting Wang, Xiaojing Wang, Zhaohua Zhou, Tong Gu, Wanjun |
author_sort | Qian, Zhongqing |
collection | PubMed |
description | BACKGROUND: Circular RNAs (circRNAs) are a class of novel RNAs with important biological functions, and aberrant expression of circRNAs has been implicated in human diseases. However, the feasibility of using blood circRNAs as disease biomarkers is largely unknown. METHODS: We explored the potential of using human peripheral blood mononuclear cell (PBMC) circRNAs as marker molecules to diagnose active pulmonary tuberculosis (TB). FINDINGS: First, we demonstrated that circRNAs are widely expressed in human PBMCs and that many are abundant enough to be detected. Second, we found that the magnitude of PBMC circRNAs in TB patients was higher than that in the paired healthy controls. Compared with host linear transcripts, the circRNAs within several pathways are disproportionately upregulated in active TB patients, including “Cytokine-cytokine receptor interaction”, “Chemokine signaling pathway”, “Neurotrophin signaling pathway”, and “Bacterial invasion of epithelial cells”. Based on the differentially expressed circRNAs within these pathways, we developed a PBMC circRNA-based molecular signature differentiating active TB patients from healthy controls. We validated the classification power of the PBMC circRNA signature in an independent cohort with the area under the receiver operating characteristic curve (AUC) at 0.946. INTERPRETATION: Our results suggest that PBMC circRNAs are potentially reliable marker molecules in TB diagnosis. |
format | Online Article Text |
id | pubmed-5828303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-58283032018-02-28 Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis Qian, Zhongqing Liu, Hui Li, Musheng Shi, Junchao Li, Na Zhang, Yao Zhang, Xiaojie Lv, Jingzhu Xie, Xueying Bai, Yunfei Ge, Qinyu Ko, Eun-A Tang, Haiyang Wang, Ting Wang, Xiaojing Wang, Zhaohua Zhou, Tong Gu, Wanjun EBioMedicine Research Paper BACKGROUND: Circular RNAs (circRNAs) are a class of novel RNAs with important biological functions, and aberrant expression of circRNAs has been implicated in human diseases. However, the feasibility of using blood circRNAs as disease biomarkers is largely unknown. METHODS: We explored the potential of using human peripheral blood mononuclear cell (PBMC) circRNAs as marker molecules to diagnose active pulmonary tuberculosis (TB). FINDINGS: First, we demonstrated that circRNAs are widely expressed in human PBMCs and that many are abundant enough to be detected. Second, we found that the magnitude of PBMC circRNAs in TB patients was higher than that in the paired healthy controls. Compared with host linear transcripts, the circRNAs within several pathways are disproportionately upregulated in active TB patients, including “Cytokine-cytokine receptor interaction”, “Chemokine signaling pathway”, “Neurotrophin signaling pathway”, and “Bacterial invasion of epithelial cells”. Based on the differentially expressed circRNAs within these pathways, we developed a PBMC circRNA-based molecular signature differentiating active TB patients from healthy controls. We validated the classification power of the PBMC circRNA signature in an independent cohort with the area under the receiver operating characteristic curve (AUC) at 0.946. INTERPRETATION: Our results suggest that PBMC circRNAs are potentially reliable marker molecules in TB diagnosis. Elsevier 2017-12-08 /pmc/articles/PMC5828303/ /pubmed/29248507 http://dx.doi.org/10.1016/j.ebiom.2017.12.007 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper Qian, Zhongqing Liu, Hui Li, Musheng Shi, Junchao Li, Na Zhang, Yao Zhang, Xiaojie Lv, Jingzhu Xie, Xueying Bai, Yunfei Ge, Qinyu Ko, Eun-A Tang, Haiyang Wang, Ting Wang, Xiaojing Wang, Zhaohua Zhou, Tong Gu, Wanjun Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis |
title | Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis |
title_full | Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis |
title_fullStr | Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis |
title_full_unstemmed | Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis |
title_short | Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis |
title_sort | potential diagnostic power of blood circular rna expression in active pulmonary tuberculosis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828303/ https://www.ncbi.nlm.nih.gov/pubmed/29248507 http://dx.doi.org/10.1016/j.ebiom.2017.12.007 |
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