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SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA
The number of known long noncoding RNA (lncRNA) functions is rapidly growing, but how those functions are encoded in their sequence and structure remains poorly understood. NORAD (noncoding RNA activated by DNA damage) is a recently characterized, abundant, and highly conserved lncRNA that is requir...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828396/ https://www.ncbi.nlm.nih.gov/pubmed/29386330 http://dx.doi.org/10.1101/gad.309138.117 |
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author | Tichon, Ailone Perry, Rotem Ben-Tov Stojic, Lovorka Ulitsky, Igor |
author_facet | Tichon, Ailone Perry, Rotem Ben-Tov Stojic, Lovorka Ulitsky, Igor |
author_sort | Tichon, Ailone |
collection | PubMed |
description | The number of known long noncoding RNA (lncRNA) functions is rapidly growing, but how those functions are encoded in their sequence and structure remains poorly understood. NORAD (noncoding RNA activated by DNA damage) is a recently characterized, abundant, and highly conserved lncRNA that is required for proper mitotic divisions in human cells. NORAD acts in the cytoplasm and antagonizes repressors from the Pumilio family that bind at least 17 sites spread through 12 repetitive units in NORAD sequence. Here we study conserved sequences in NORAD repeats, identify additional interacting partners, and characterize the interaction between NORAD and the RNA-binding protein SAM68 (KHDRBS1), which is required for NORAD function in antagonizing Pumilio. These interactions provide a paradigm for how repeated elements in a lncRNA facilitate function. |
format | Online Article Text |
id | pubmed-5828396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-58283962018-07-01 SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA Tichon, Ailone Perry, Rotem Ben-Tov Stojic, Lovorka Ulitsky, Igor Genes Dev Research Paper The number of known long noncoding RNA (lncRNA) functions is rapidly growing, but how those functions are encoded in their sequence and structure remains poorly understood. NORAD (noncoding RNA activated by DNA damage) is a recently characterized, abundant, and highly conserved lncRNA that is required for proper mitotic divisions in human cells. NORAD acts in the cytoplasm and antagonizes repressors from the Pumilio family that bind at least 17 sites spread through 12 repetitive units in NORAD sequence. Here we study conserved sequences in NORAD repeats, identify additional interacting partners, and characterize the interaction between NORAD and the RNA-binding protein SAM68 (KHDRBS1), which is required for NORAD function in antagonizing Pumilio. These interactions provide a paradigm for how repeated elements in a lncRNA facilitate function. Cold Spring Harbor Laboratory Press 2018-01-01 /pmc/articles/PMC5828396/ /pubmed/29386330 http://dx.doi.org/10.1101/gad.309138.117 Text en © 2018 Tichon et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Paper Tichon, Ailone Perry, Rotem Ben-Tov Stojic, Lovorka Ulitsky, Igor SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA |
title | SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA |
title_full | SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA |
title_fullStr | SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA |
title_full_unstemmed | SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA |
title_short | SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA |
title_sort | sam68 is required for regulation of pumilio by the norad long noncoding rna |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828396/ https://www.ncbi.nlm.nih.gov/pubmed/29386330 http://dx.doi.org/10.1101/gad.309138.117 |
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