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Electrofusion of single cells in picoliter droplets

We present a microfluidic chip that enables electrofusion of cells in microdroplets, with exchange of nuclear components. It is shown, to our knowledge for the first time, electrofusion of two HL60 cells, inside a microdroplet. This is the crucial intermediate step for controlled hybridoma formation...

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Detalles Bibliográficos
Autores principales:	Schoeman, Rogier M., van den Beld, Wesley T. E., Kemna, Evelien W. M., Wolbers, Floor, Eijkel, Jan C. T., van den Berg, Albert
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829161/ https://www.ncbi.nlm.nih.gov/pubmed/29487332 http://dx.doi.org/10.1038/s41598-018-21993-8

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