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Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere

Here we report the discovery of two Tupanvirus strains, the longest tailed Mimiviridae members isolated in amoebae. Their genomes are 1.44–1.51 Mb linear double-strand DNA coding for 1276–1425 predicted proteins. Tupanviruses share the same ancestors with mimivirus lineages and these giant viruses p...

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Autores principales: Abrahão, Jônatas, Silva, Lorena, Silva, Ludmila Santos, Khalil, Jacques Yaacoub Bou, Rodrigues, Rodrigo, Arantes, Thalita, Assis, Felipe, Boratto, Paulo, Andrade, Miguel, Kroon, Erna Geessien, Ribeiro, Bergmann, Bergier, Ivan, Seligmann, Herve, Ghigo, Eric, Colson, Philippe, Levasseur, Anthony, Kroemer, Guido, Raoult, Didier, La Scola, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829246/
https://www.ncbi.nlm.nih.gov/pubmed/29487281
http://dx.doi.org/10.1038/s41467-018-03168-1
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author Abrahão, Jônatas
Silva, Lorena
Silva, Ludmila Santos
Khalil, Jacques Yaacoub Bou
Rodrigues, Rodrigo
Arantes, Thalita
Assis, Felipe
Boratto, Paulo
Andrade, Miguel
Kroon, Erna Geessien
Ribeiro, Bergmann
Bergier, Ivan
Seligmann, Herve
Ghigo, Eric
Colson, Philippe
Levasseur, Anthony
Kroemer, Guido
Raoult, Didier
La Scola, Bernard
author_facet Abrahão, Jônatas
Silva, Lorena
Silva, Ludmila Santos
Khalil, Jacques Yaacoub Bou
Rodrigues, Rodrigo
Arantes, Thalita
Assis, Felipe
Boratto, Paulo
Andrade, Miguel
Kroon, Erna Geessien
Ribeiro, Bergmann
Bergier, Ivan
Seligmann, Herve
Ghigo, Eric
Colson, Philippe
Levasseur, Anthony
Kroemer, Guido
Raoult, Didier
La Scola, Bernard
author_sort Abrahão, Jônatas
collection PubMed
description Here we report the discovery of two Tupanvirus strains, the longest tailed Mimiviridae members isolated in amoebae. Their genomes are 1.44–1.51 Mb linear double-strand DNA coding for 1276–1425 predicted proteins. Tupanviruses share the same ancestors with mimivirus lineages and these giant viruses present the largest translational apparatus within the known virosphere, with up to 70 tRNA, 20 aaRS, 11 factors for all translation steps, and factors related to tRNA/mRNA maturation and ribosome protein modification. Moreover, two sequences with significant similarity to intronic regions of 18 S rRNA genes are encoded by the tupanviruses and highly expressed. In this translation-associated gene set, only the ribosome is lacking. At high multiplicity of infections, tupanvirus is also cytotoxic and causes a severe shutdown of ribosomal RNA and a progressive degradation of the nucleus in host and non-host cells. The analysis of tupanviruses constitutes a new step toward understanding the evolution of giant viruses.
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spelling pubmed-58292462018-03-02 Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere Abrahão, Jônatas Silva, Lorena Silva, Ludmila Santos Khalil, Jacques Yaacoub Bou Rodrigues, Rodrigo Arantes, Thalita Assis, Felipe Boratto, Paulo Andrade, Miguel Kroon, Erna Geessien Ribeiro, Bergmann Bergier, Ivan Seligmann, Herve Ghigo, Eric Colson, Philippe Levasseur, Anthony Kroemer, Guido Raoult, Didier La Scola, Bernard Nat Commun Article Here we report the discovery of two Tupanvirus strains, the longest tailed Mimiviridae members isolated in amoebae. Their genomes are 1.44–1.51 Mb linear double-strand DNA coding for 1276–1425 predicted proteins. Tupanviruses share the same ancestors with mimivirus lineages and these giant viruses present the largest translational apparatus within the known virosphere, with up to 70 tRNA, 20 aaRS, 11 factors for all translation steps, and factors related to tRNA/mRNA maturation and ribosome protein modification. Moreover, two sequences with significant similarity to intronic regions of 18 S rRNA genes are encoded by the tupanviruses and highly expressed. In this translation-associated gene set, only the ribosome is lacking. At high multiplicity of infections, tupanvirus is also cytotoxic and causes a severe shutdown of ribosomal RNA and a progressive degradation of the nucleus in host and non-host cells. The analysis of tupanviruses constitutes a new step toward understanding the evolution of giant viruses. Nature Publishing Group UK 2018-02-27 /pmc/articles/PMC5829246/ /pubmed/29487281 http://dx.doi.org/10.1038/s41467-018-03168-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Abrahão, Jônatas
Silva, Lorena
Silva, Ludmila Santos
Khalil, Jacques Yaacoub Bou
Rodrigues, Rodrigo
Arantes, Thalita
Assis, Felipe
Boratto, Paulo
Andrade, Miguel
Kroon, Erna Geessien
Ribeiro, Bergmann
Bergier, Ivan
Seligmann, Herve
Ghigo, Eric
Colson, Philippe
Levasseur, Anthony
Kroemer, Guido
Raoult, Didier
La Scola, Bernard
Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere
title Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere
title_full Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere
title_fullStr Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere
title_full_unstemmed Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere
title_short Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere
title_sort tailed giant tupanvirus possesses the most complete translational apparatus of the known virosphere
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829246/
https://www.ncbi.nlm.nih.gov/pubmed/29487281
http://dx.doi.org/10.1038/s41467-018-03168-1
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