Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency

OCT4 is required to maintain the pluripotency of embryonic stem cells (ESCs); yet, overdose-expression of OCT4 induces ESC differentiation toward primitive endoderm. The molecular mechanism underlying this differentiation switch is not fully understood. Here, we found that substitution of threonine(...

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Autores principales: Abulaiti, Xianmixinuer, Zhang, Han, Wang, Aifang, Li, Na, Li, Yang, Wang, Chenchen, Du, Xiaojuan, Li, Lingsong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829306/
https://www.ncbi.nlm.nih.gov/pubmed/28988986
http://dx.doi.org/10.1016/j.stemcr.2017.09.001
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author Abulaiti, Xianmixinuer
Zhang, Han
Wang, Aifang
Li, Na
Li, Yang
Wang, Chenchen
Du, Xiaojuan
Li, Lingsong
author_facet Abulaiti, Xianmixinuer
Zhang, Han
Wang, Aifang
Li, Na
Li, Yang
Wang, Chenchen
Du, Xiaojuan
Li, Lingsong
author_sort Abulaiti, Xianmixinuer
collection PubMed
description OCT4 is required to maintain the pluripotency of embryonic stem cells (ESCs); yet, overdose-expression of OCT4 induces ESC differentiation toward primitive endoderm. The molecular mechanism underlying this differentiation switch is not fully understood. Here, we found that substitution of threonine(343) by alanine (T343A), but not aspartic acid (T343D), caused a significant loss of OCT4-phosphorylation signal in ESCs. Loss of such OCT4-phosphorylation compromises its interaction with SOX2 but promotes interaction with SOX17. We therefore propose that threonine(343)-based OCT4-phosphorylation is crucial for the maintenance of ESC pluripotency. This OCT4-phosphorylation-based mechanism may provide insight into the regulation of lineage specification during early embryonic development.
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spelling pubmed-58293062018-02-28 Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency Abulaiti, Xianmixinuer Zhang, Han Wang, Aifang Li, Na Li, Yang Wang, Chenchen Du, Xiaojuan Li, Lingsong Stem Cell Reports Article OCT4 is required to maintain the pluripotency of embryonic stem cells (ESCs); yet, overdose-expression of OCT4 induces ESC differentiation toward primitive endoderm. The molecular mechanism underlying this differentiation switch is not fully understood. Here, we found that substitution of threonine(343) by alanine (T343A), but not aspartic acid (T343D), caused a significant loss of OCT4-phosphorylation signal in ESCs. Loss of such OCT4-phosphorylation compromises its interaction with SOX2 but promotes interaction with SOX17. We therefore propose that threonine(343)-based OCT4-phosphorylation is crucial for the maintenance of ESC pluripotency. This OCT4-phosphorylation-based mechanism may provide insight into the regulation of lineage specification during early embryonic development. Elsevier 2017-10-05 /pmc/articles/PMC5829306/ /pubmed/28988986 http://dx.doi.org/10.1016/j.stemcr.2017.09.001 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Abulaiti, Xianmixinuer
Zhang, Han
Wang, Aifang
Li, Na
Li, Yang
Wang, Chenchen
Du, Xiaojuan
Li, Lingsong
Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
title Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
title_full Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
title_fullStr Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
title_full_unstemmed Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
title_short Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency
title_sort phosphorylation of threonine(343) is crucial for oct4 interaction with sox2 in the maintenance of mouse embryonic stem cell pluripotency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829306/
https://www.ncbi.nlm.nih.gov/pubmed/28988986
http://dx.doi.org/10.1016/j.stemcr.2017.09.001
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