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i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions

i-Motif (iM) is a four stranded DNA structure formed by cytosine-rich sequences, which are often present in functionally important parts of the genome such as promoters of genes and telomeres. Using electronic circular dichroism and UV absorption spectroscopies and electrophoretic methods, we examin...

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Autores principales: Dvořáková, Zuzana, Renčiuk, Daniel, Kejnovská, Iva, Školáková, Petra, Bednářová, Klára, Sagi, Janos, Vorlíčková, Michaela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829569/
https://www.ncbi.nlm.nih.gov/pubmed/29378012
http://dx.doi.org/10.1093/nar/gky035
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author Dvořáková, Zuzana
Renčiuk, Daniel
Kejnovská, Iva
Školáková, Petra
Bednářová, Klára
Sagi, Janos
Vorlíčková, Michaela
author_facet Dvořáková, Zuzana
Renčiuk, Daniel
Kejnovská, Iva
Školáková, Petra
Bednářová, Klára
Sagi, Janos
Vorlíčková, Michaela
author_sort Dvořáková, Zuzana
collection PubMed
description i-Motif (iM) is a four stranded DNA structure formed by cytosine-rich sequences, which are often present in functionally important parts of the genome such as promoters of genes and telomeres. Using electronic circular dichroism and UV absorption spectroscopies and electrophoretic methods, we examined the effect of four naturally occurring DNA base lesions on the folding and stability of the iM formed by the human telomere DNA sequence (C(3)TAA)(3)C(3)T. The results demonstrate that the TAA loop lesions, the apurinic site and 8-oxoadenine substituting for adenine, and the 5-hydroxymethyluracil substituting for thymine only marginally disturb the formation of iM. The presence of uracil, which is formed by enzymatic or spontaneous deamination of cytosine, shifts iM formation towards substantially more acidic pH values and simultaneously distinctly reduces iM stability. This effect depends on the position of the damage sites in the sequence. The results have enabled us to formulate additional rules for iM formation.
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spelling pubmed-58295692018-03-06 i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions Dvořáková, Zuzana Renčiuk, Daniel Kejnovská, Iva Školáková, Petra Bednářová, Klára Sagi, Janos Vorlíčková, Michaela Nucleic Acids Res Chemical Biology and Nucleic Acid Chemistry i-Motif (iM) is a four stranded DNA structure formed by cytosine-rich sequences, which are often present in functionally important parts of the genome such as promoters of genes and telomeres. Using electronic circular dichroism and UV absorption spectroscopies and electrophoretic methods, we examined the effect of four naturally occurring DNA base lesions on the folding and stability of the iM formed by the human telomere DNA sequence (C(3)TAA)(3)C(3)T. The results demonstrate that the TAA loop lesions, the apurinic site and 8-oxoadenine substituting for adenine, and the 5-hydroxymethyluracil substituting for thymine only marginally disturb the formation of iM. The presence of uracil, which is formed by enzymatic or spontaneous deamination of cytosine, shifts iM formation towards substantially more acidic pH values and simultaneously distinctly reduces iM stability. This effect depends on the position of the damage sites in the sequence. The results have enabled us to formulate additional rules for iM formation. Oxford University Press 2018-02-28 2018-01-25 /pmc/articles/PMC5829569/ /pubmed/29378012 http://dx.doi.org/10.1093/nar/gky035 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Chemical Biology and Nucleic Acid Chemistry
Dvořáková, Zuzana
Renčiuk, Daniel
Kejnovská, Iva
Školáková, Petra
Bednářová, Klára
Sagi, Janos
Vorlíčková, Michaela
i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
title i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
title_full i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
title_fullStr i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
title_full_unstemmed i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
title_short i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
title_sort i-motif of cytosine-rich human telomere dna fragments containing natural base lesions
topic Chemical Biology and Nucleic Acid Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829569/
https://www.ncbi.nlm.nih.gov/pubmed/29378012
http://dx.doi.org/10.1093/nar/gky035
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