Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties

Nonmusclemyosin 2 (NM-2) powers cell motility and tissue morphogenesis by assembling into bipolar filaments that interact with actin. Although the enzymatic properties of purified NM-2 motor fragments have been determined, the emergent properties of filament ensembles are unknown. Using single myosi...

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Autores principales: Melli, Luca, Billington, Neil, Sun, Sara A, Bird, Jonathan E, Nagy, Attila, Friedman, Thomas B, Takagi, Yasuharu, Sellers, James R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829915/
https://www.ncbi.nlm.nih.gov/pubmed/29419377
http://dx.doi.org/10.7554/eLife.32871
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author Melli, Luca
Billington, Neil
Sun, Sara A
Bird, Jonathan E
Nagy, Attila
Friedman, Thomas B
Takagi, Yasuharu
Sellers, James R
author_facet Melli, Luca
Billington, Neil
Sun, Sara A
Bird, Jonathan E
Nagy, Attila
Friedman, Thomas B
Takagi, Yasuharu
Sellers, James R
author_sort Melli, Luca
collection PubMed
description Nonmusclemyosin 2 (NM-2) powers cell motility and tissue morphogenesis by assembling into bipolar filaments that interact with actin. Although the enzymatic properties of purified NM-2 motor fragments have been determined, the emergent properties of filament ensembles are unknown. Using single myosin filament in vitro motility assays, we report fundamental differences in filaments formed of different NM-2 motors. Filaments consisting of NM2-B moved processively along actin, while under identical conditions, NM2-A filaments did not. By more closely mimicking the physiological milieu, either by increasing solution viscosity or by co-polymerization with NM2-B, NM2-A containing filaments moved processively. Our data demonstrate that both the kinetic and mechanical properties of these two myosins, in addition to the stochiometry of NM-2 subunits, can tune filament mechanical output. We propose altering NM-2 filament composition is a general cellular strategy for tailoring force production of filaments to specific functions, such as maintaining tension or remodeling actin.
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spelling pubmed-58299152018-03-05 Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties Melli, Luca Billington, Neil Sun, Sara A Bird, Jonathan E Nagy, Attila Friedman, Thomas B Takagi, Yasuharu Sellers, James R eLife Structural Biology and Molecular Biophysics Nonmusclemyosin 2 (NM-2) powers cell motility and tissue morphogenesis by assembling into bipolar filaments that interact with actin. Although the enzymatic properties of purified NM-2 motor fragments have been determined, the emergent properties of filament ensembles are unknown. Using single myosin filament in vitro motility assays, we report fundamental differences in filaments formed of different NM-2 motors. Filaments consisting of NM2-B moved processively along actin, while under identical conditions, NM2-A filaments did not. By more closely mimicking the physiological milieu, either by increasing solution viscosity or by co-polymerization with NM2-B, NM2-A containing filaments moved processively. Our data demonstrate that both the kinetic and mechanical properties of these two myosins, in addition to the stochiometry of NM-2 subunits, can tune filament mechanical output. We propose altering NM-2 filament composition is a general cellular strategy for tailoring force production of filaments to specific functions, such as maintaining tension or remodeling actin. eLife Sciences Publications, Ltd 2018-02-08 /pmc/articles/PMC5829915/ /pubmed/29419377 http://dx.doi.org/10.7554/eLife.32871 Text en http://creativecommons.org/publicdomain/zero/1.0/ http://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (http://creativecommons.org/publicdomain/zero/1.0/) .
spellingShingle Structural Biology and Molecular Biophysics
Melli, Luca
Billington, Neil
Sun, Sara A
Bird, Jonathan E
Nagy, Attila
Friedman, Thomas B
Takagi, Yasuharu
Sellers, James R
Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties
title Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties
title_full Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties
title_fullStr Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties
title_full_unstemmed Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties
title_short Bipolar filaments of human nonmuscle myosin 2-A and 2-B have distinct motile and mechanical properties
title_sort bipolar filaments of human nonmuscle myosin 2-a and 2-b have distinct motile and mechanical properties
topic Structural Biology and Molecular Biophysics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829915/
https://www.ncbi.nlm.nih.gov/pubmed/29419377
http://dx.doi.org/10.7554/eLife.32871
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