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‘Cyclical Bias’ in Microbiome Research Revealed by A Portable Germ-Free Housing System Using Nested Isolation
Germ-Free (GF) research has required highly technical pressurized HEPA-ventilation anchored systems for decades. Herein, we validated a GF system that can be easily implemented and portable using Nested Isolation (NesTiso). GF-standards can be achieved housing mice in non-HEPA-static cages, which on...
Autores principales: | Rodriguez-Palacios, Alexander, Aladyshkina, Natalia, Ezeji, Jessica C., Erkkila, Hailey L., Conger, Mathew, Ward, John, Webster, Joshua, Cominelli, Fabio |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830500/ https://www.ncbi.nlm.nih.gov/pubmed/29491439 http://dx.doi.org/10.1038/s41598-018-20742-1 |
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